Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements

2002 ◽  
Vol 1 (3) ◽  
pp. 450-451 ◽  
Author(s):  
Lori Beach ◽  
Claude Schweitzer ◽  
J. C. Scaiano
Keyword(s):  
Steady State ◽  
Direct Determination ◽  
Steady State Fluorescence ◽  
Fluorescence Measurements ◽  
Double Stranded Dna

scholarly journals Determination of Enantiomeric Compositions of Analytes Using Novel Fluorescent Chiral Molecular Micelles and Steady State Fluorescence Measurements

2007 ◽  
Vol 18 (2) ◽  
pp. 285-296 ◽  
Author(s):  
Alicia A. Williams ◽  
Sayo O. Fakayode ◽  
Onur Alptürk ◽  
Christina M. Jones ◽  
Mark Lowry ◽  
...  
Keyword(s):  
Steady State ◽  
Steady State Fluorescence ◽  
Fluorescence Measurements

scholarly journals Steady State Response of Linear Time Invariant Systems Modeledby Multibond Graphs

2021 ◽  
Vol 11 (4) ◽  
pp. 1717
Author(s):  
Gilberto Gonzalez Avalos ◽  
Noe Barrera Gallegos ◽  
Gerardo Ayala-Jaimes ◽  
Aaron Padilla Garcia
Keyword(s):  
Steady State ◽  
Linear Time ◽  
Direct Determination ◽  
The State ◽  
Steady State Response ◽  
State Variables ◽  
Linear Time Invariant ◽  
State Response ◽  
Time Invariant

The direct determination of the steady state response for linear time invariant (LTI) systems modeled by multibond graphs is presented. Firstly, a multiport junction structure of a multibond graph in an integral causality assignment (MBGI) to get the state space of the system is introduced. By assigning a derivative causality to the multiport storage elements, the multibond graph in a derivative causality (MBGD) is proposed. Based on this MBGD, a theorem to obtain the steady state response is presented. Two case studies to get the steady state of the state variables are applied. Both cases are modeled by multibond graphs, and the symbolic determination of the steady state is obtained. The simulation results using the 20-SIM software are numerically verified.

scholarly journals Determination of Critical Micellar Aggregation Numbers by Steadystate Fluorescence Probe Method

2001 ◽  
Vol 17 (09) ◽  
pp. 828-831 ◽  
Author(s):  
Fang Yun ◽  
◽  
Liu Xue-Feng ◽  
Xia Yong-Mei ◽  
Yang Yang ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorescence Probe ◽  
Probe Method ◽  
Steady State Fluorescence ◽  
Aggregation Numbers

Ultrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes

2004 ◽  
Vol 6 (1) ◽  
pp. 154 ◽  
Author(s):  
Olaf F. A. Larsen ◽  
Ivo H. M. van Stokkum ◽  
Frank L. de Weerd ◽  
Mikas Vengris ◽  
Charuvila T. Aravindakumar ◽  
...  
Keyword(s):  
Steady State ◽  
Transient Absorption ◽  
Dna Duplexes ◽  
Steady State Fluorescence ◽  
Fluorescence Measurements ◽  
Ultrafast Transient Absorption

Chloroplast Cytochrome b6/f and ATP Synthase Complexes in Tobacco: Transformation With Antisense RNA Against Nuclear-Encoded Transcripts for the Rieske FeS and ATPδ Polypeptides

1995 ◽  
Vol 22 (2) ◽  
pp. 285 ◽  
Author(s):  
GD Price ◽  
JW Yu ◽  
SV Caemmerer ◽  
JR Evans ◽  
WS Chow ◽  
...  
Keyword(s):  
Steady State ◽  
Atp Synthase ◽  
Antisense Rna ◽  
Photochemical Quenching ◽  
Cdna Clones ◽  
Wild Type ◽  
Cytochrome B6 ◽  
Steady State Fluorescence ◽  
Fluorescence Measurements ◽  
Non Photochemical Quenching

Tobacco plants with reduced amounts and activities of both the chloroplast cytochrome b6/f and ATP synthase complexes have been produced using antisense RNA techniques. Antisense constructs were generated from tobacco cDNA clones coding for the Rieske FeS protein and the δ subunit of the b6/f and ATP synthase complexes respectively. Transformants with altered activities were selected using pulse-modulated fluorescence measurements. The b6/ftransformants showed high levels of steady-state fluorescence and reduced levels of both photochemical and non-photochemical quenching. In striking contrast, the ATP synthase transformants showed low levels of steady-state fluorescence and greatly increased levels of non-photochemical quenching. Transformants with a range of suppression were isolated for both constructs, in some cases with photosynthesis reduced to less than 10% of wild-type values. The most severely affected transformants showed extremely slow growth and in some cases they were unable to grow and produce seed. Progeny from the R1 seed from several cytochrome b6/f transformants have been analysed and show segregation of phenotypes ranging from intermediate to severe in repression. Intermediate and severe phenotype plants showed a reduction in Rieske FeS mRNA of more than 90% while FeS polypeptide was reduced to 60 and 86% of wild type. There was a strong correlation between photosynthesis at air and Rieske FeS polypeptide content in the antisense plants suggesting that the cytochrome b6/f complex was a major determinant of photosynthetic rate under these conditions. Photoinhibition studies of FeS antisense plants showed that there was a reduced activity of the xanthophyll cycle in the most severe plants, consistent with a reduction in the transthylakoid pH gradient and a lowered non-photochemical quenching. Preliminary studies of the ATPδ antisense plants showed that they also had reduced levels of mRNA and ATPδ polypeptide.

Sign in / Sign up

Export Citation Format

Share Document