Isolation and cytogenetic analyses of genetic sexing strains for the medfly, Ceratitis capitata

1995 ◽  
Vol 91 (2) ◽  
pp. 255-261 ◽  
Author(s):  
P. Kerremans ◽  
G. Franz
Keyword(s):  
Ceratitis Capitata ◽  
Genetic Sexing ◽  
Cytogenetic Analyses ◽  
Genetic Sexing Strains

scholarly journals Genetic analysis of medfly populations in an area of sterile insect technique applications

2021 ◽  
Author(s):  
Rubén Sancho ◽  
Ana Guillem-Amat ◽  
Elena López-Errasquín ◽  
Lucas Sánchez ◽  
Félix Ortego ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Sterile Insect Technique ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Low Frequency ◽  
Fruit Fly ◽  
Wild Populations ◽  
Genetic Sexing ◽  
Environmental Implications ◽  
Genetic Sexing Strains

AbstractThe sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The genetic interactions between the released individuals from the genetic sexing strains (GSS), used for SIT applications worldwide, and wild individuals have not been studied. Under the hypothesis that a number of Vienna GSS individuals released to the field might not be completely sterile and may produce viable offspring, we have analyzed medfly Spanish field populations to evaluate the presence of Vienna strain genetic markers. To this goal, we have used contrasted nuclear and mitochondrial genetic markers, and two novel sets of nuclear polymorphisms with the potential to be markers to discriminate between Vienna and wild individuals. Nuclear Vienna markers located on the 5th chromosome of Vienna males have been found in 2.2% (19 from 875) of the Spanish wild medfly females captured at the area where SIT is applied. In addition, a female-inherited mitochondrial Vienna marker has been found in two from the 19 females showing nuclear Vienna markers. The detection of several of these markers in single individuals represents evidence of the introgression of Vienna strain into natural populations. However, alternative explanations as their presence at low frequency in wild populations in the studied areas cannot be fully discarded. The undesired release of non-fully sterile irradiated GSS individuals into the field and their interactions with wild flies, and the potential environmental implications should be taken into account in the application of the SIT.

scholarly journals Development, genetic and cytogenetic analyses of genetic sexing strains of the Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae)

BMC Genetics ◽  
2014 ◽  
Vol 15 (Suppl 2) ◽  
pp. S1 ◽  
Author(s):  
Cristina Silvia Zepeda-Cisneros ◽  
José Salvador Meza Hernández ◽  
Víctor García-Martínez ◽  
Jorge Ibañez-Palacios ◽  
Antigone Zacharopoulou ◽  
...  
Keyword(s):  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Genetic Sexing ◽  
Cytogenetic Analyses ◽  
Genetic Sexing Strains

Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 72-82 ◽  
Author(s):  
G. Franz ◽  
E. Gencheva ◽  
Ph. Kerremans
Keyword(s):  
Ceratitis Capitata ◽  
Recombination Frequency ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Temperature Sensitive ◽  
Genetic Sexing ◽  
Male Recombination ◽  
Comparable Level ◽  
Improved Stability ◽  
Genetic Sexing Strains

In the existing genetic sexing strains for the medfly, Ceratitis capitata, male recombination leads to breakdown of the sexing mechanism under mass rearing conditions. The rate of breakdown depends on the recombination frequency and on the fitness of the recombinants. We have tested two different sexing genes, white pupa and a temperature sensitive lethal, in combination with the translocation T(Y;5)30C. Both sexing strains broke down, although at very different rates. In the case of the white pupa strain, 3.5% recombinants were observed after rearing the strain for 15 generations. The second strain, utilizing white pupa and the temperature sensitive lethal as selectable markers, already reached a comparable level after six generations and was broken down completely in the ninth generation. In these strains the frequency of recombination is high because the breakpoint of T(Y;5)30C and the sexing gene(s) are far apart. To remedy the situation, we have isolated four new translocations with breakpoints located closer to the sexing genes. Mass rearing was simulated for several generations with strains based on these translocations and no breakdown was observed under the conditions used.Key words: medfly, sterile insect technique, genetic sexing, recombination.

scholarly journals Ceratitis capitata genetic sexing strains: laboratory evaluation of strains from mass-rearing facilities worldwide

2017 ◽  
Vol 164 (3) ◽  
pp. 305-317 ◽  
Author(s):  
A.A. Augustinos ◽  
A. Targovska ◽  
E. Cancio-Martinez ◽  
E. Schorn ◽  
G. Franz ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Mass Rearing ◽  
Laboratory Evaluation ◽  
Genetic Sexing ◽  
Genetic Sexing Strains

scholarly journals Pupation Substrate Type and Volume Affect Pupation, Quality Parameters and Production Costs of a Reproductive Colony of Ceratitis capitata (Diptera: Tephritidae) VIENNA 8 Genetic Sexing Strain

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 337
Author(s):  
Carlos Pascacio-Villafán ◽  
Luis Quintero-Fong ◽  
Larissa Guillén ◽  
José Pedro Rivera-Ciprian ◽  
Reynaldo Aguilar ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Ceratitis Capitata ◽  
Mass Rearing ◽  
Physicochemical Characteristics ◽  
Production Costs ◽  
Cellulose I ◽  
Coconut Fiber ◽  
Genetic Sexing ◽  
Recycled Paper ◽  
Genetic Sexing Strain

Adequate pupation substrates and substrate volume are critical factors in the mass-rearing of insects for Sterile Insect Technique (SIT) applications. To identify an ideal pupation substrate for a reproductive colony of Ceratitis capitata (Wiedemann) VIENNA 8 genetic sexing strain, we first examined pupation in cellulose from recycled paper (cellulose I), sawdust, fine wheat bran, vermiculite and coconut fiber using a volume of 2.5–12.5 mL of substrate for each 5 mL volume of fly larvae. We found a positive relationship between substrate volume and pupation, with cellulose I generating the highest proportions of pupation and coconut fiber the lowest. Higher proportions of female flies (white pupae) pupated in sawdust. The proportion of female fliers increased as substrate volume rose in sawdust and coconut fiber, whereas it decreased in vermiculite and cellulose. In a second experiment, we tested three types of cellulose differing in physicochemical characteristics (celluloses I, II and III), sawdust, and fine wheat bran using a substrate:larvae ratio of 1:1. The three types of cellulose produced the highest pupation levels. The highest proportions of female fliers were observed in sawdust, and cellulose types III and II. Cellulose III and sawdust at relatively low volumes were more cost-effective to produce one million pupae than other substrates, including fine wheat bran used in a mass-rearing facility in Mexico.

Simulation of instability in genetic sexing strains: effects of male recombination in association with other biological parameters

1991 ◽  
Vol 81 (1) ◽  
pp. 11-20 ◽  
Author(s):  
E. Busch-Petersen ◽  
H. Baumgartner
Keyword(s):  
Genetic Recombination ◽  
Mass Rearing ◽  
Biological Parameters ◽  
Genetic Sexing ◽  
Male Recombination ◽  
Rate Of Progression ◽  
Control Programmes ◽  
Males And Females ◽  
Low Levels ◽  
Genetic Sexing Strains

AbstractGenetic systems have been developed in several insect species for separating males and females prior to releasing sterilized males in pest control programmes using the sterile insect technique. The systems generally depend on translocating a readily selectable gene onto the Y chromosome. A potential source of instability in such a system is genetic recombination in the male. Although such recombination was originally thought to be absent in most cyclorrhaphous Diptera, low levels have recently been found. We have developed a computer model which simulates the progression of instability in the presence of male recombination, which can be used to assess the influence of rate of recombination in combination with a range of associated genetic and biological parameters. Male recombination alone or fitness of the Y-linked translocation were found to contribute relatively little to the rate of progression of instability. By contrast reduced fitness or mating competitiveness associated with the selectable gene had a strong effect. The sex ratio and the ratio of carriers to non-carriers of the selectable gene showed patterns characteristic of the parameters modelled. The relevance of such data to the development of suitable strains for genetic sex-separation and the replacement of strains under mass rearing conditions are discussed.

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