Characterization of a highly repeated DNA component of perennial oats (Helictotrichon, Poaceae) with sequence similarity to a A-genome-specific satellite DNA of rice (Oryza)

Satellite DNA specific to the oat C genome was sequenced and located on chromosomes of diploid, tetraploid, and hexaploid Avena ssp. using in situ hybridization. The sequence was present on all seven C genome chromosome pairs and hybridized to the entire length of each chromosome, with the exception of the terminal segments of some chromosome pairs. Three chromosome pairs belonging to the A genome showed hybridization signals near the telomeres of their long arms. The existence of intergenomic chromosome rearrangements and the deletions of the repeated units are deduced from these observations. The number of rDNA loci (18S–5.8S–26S rDNA) was determined for the tetraploid and hexaploid oat species. Simultaneous in situ hybridization with the satellite and rDNA probes was used to assign the SAT chromosomes of these species to their correct genomes.Key words: oats, satellite DNA, rDNA, in situ hybridization, genome evolution.

Download Full-text

Cytogenetic and molecular characterization of a highly repeated DNA sequence in the peach potato aphid Myzus persicae

Chromosoma ◽

10.1007/s004120050395 ◽

1999 ◽

Vol 108 (7) ◽

pp. 436-442 ◽

Cited By ~ 40

Author(s):

M. Mandrioli ◽

D. Bizzaro ◽

G. C. Manicardi ◽

D. Gionghi ◽

L. Bassoli ◽

...

Keyword(s):

Molecular Characterization ◽

Dna Sequence ◽

Myzus Persicae ◽

Repeated Dna ◽

Potato Aphid ◽

Highly Repeated Dna ◽

Repeated Dna Sequence ◽

Peach Potato Aphid

Download Full-text

Distribution and complex organization of satellite DNA sequences in Aveneae species

Genome ◽

10.1139/g96-131 ◽

1996 ◽

Vol 39 (6) ◽

pp. 1045-1050 ◽

Cited By ~ 19

Author(s):

Bärbel Grebenstein ◽

Oliver Grebenstein ◽

Wilhelm Sauer ◽

Vera Hemleben

Keyword(s):

Repetitive Dna ◽

Dna Sequences ◽

Satellite Dna ◽

Sequence Similarity ◽

Satellite Dnas ◽

A Genome ◽

Complex Organization ◽

Dna Elements ◽

Taxonomic Groups ◽

Dna Components

Distribution, organization, and molecular analysis of four unrelated satellite DNA components in Aveneae species are described. Highly repeated DNA elements were cloned from Helictotrichon convolutum (CON1 and CON2) and Helictotrichon compression (COM1 and COM2). The lengths of the repeat monomers are 365 bp (CON1), 562 bp (CON2), 346 bp (COM1), and 476 bp (COM2). Similar repeats were detected by dot blots, Southern blots, and by DNA sequencing in other species of the genus Helictotrichon, in Aveneae species, and in species of the tribes Andropogoneae and Oryzeae. All four satellite DNAs are differently distributed in the taxonomic groups mentioned above. Remarkably, the longer elements are built up in a complex pattern of either shorter subrepeats arranged in tandem (COM2) or by duplications inserted into an original 369-bp element (CON2). Shorter representatives, 190 bp, similar to CON1 elements occur in Holcus species. In Koeleria species, COM1-related repeats are only 180 bp in length. No similarity was found among the sequences CON2, COM1, and COM2 or with sequences of other repetitive DNA elements of the grasses, but CON1 shows sequence similarity to an A genome specific repetitive DNA of Oryza (rice). Key words : genome evolution, grasses, Poaceae, repetitive DNA, wild oats.

Download Full-text

Characterization of a Highly Repeated DNA Family in Tapetinae Species (Mollusca Bivalvia: Veneridae)

ZOOLOGICAL SCIENCE ◽

10.2108/0289-0003(1998)15[599:coahrd]2.0.co;2 ◽

1998 ◽

Vol 15 (4) ◽

pp. 599-605 ◽

Cited By ~ 2

Author(s):

Marco Passamonti ◽

Barbara Mantovani ◽

Valerio Scali

Keyword(s):

Repeated Dna ◽

Highly Repeated Dna

Download Full-text

Characterization of a highly repeated DNA sequence (SC1) from the arbuscular mycorrhizal fungus Scutellospora castanea and its detection in planta.

Applied and Environmental Microbiology ◽

10.1128/aem.62.7.2443-2448.1996 ◽

1996 ◽

Vol 62 (7) ◽

pp. 2443-2448 ◽

Cited By ~ 36

Author(s):

A Zeze ◽

M Hosny ◽

V Gianinazzi-Pearson ◽

H Dulieu

Keyword(s):

Dna Sequence ◽

Arbuscular Mycorrhizal Fungus ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Repeated Dna ◽

In Planta ◽

Highly Repeated Dna ◽

Repeated Dna Sequence

Download Full-text

Highly repeated DNA and holocentric chromosomes of the woodrush Luzula flaccida (Juncaceae)

Canadian Journal of Genetics and Cytology ◽

10.1139/g84-045 ◽

1984 ◽

Vol 26 (3) ◽

pp. 288-295 ◽

Cited By ~ 5

Author(s):

Chris Collet

Keyword(s):

Satellite Dna ◽

Unit Length ◽

Buoyant Density ◽

Holocentric Chromosomes ◽

Repeated Dna ◽

Base Pairs ◽

Highly Repeated Dna ◽

Minor Satellite ◽

Total Dna ◽

A Minor

Three highly repeated DNA components were characterized from Luzula flaccida. The 1.683 g/cm3 satellite accounts for 22% of the genome and has a sequence complexity of 127 base pairs (BP). A further 8% of the genome consists of a cryptic satellite DNA with a repeating unit of 190 BP. A minor satellite component of buoyant density 1.681 g/cm3 constitutes less than 2% of the total DNA and has a unit length of 184 BP. The presence of segments of the 1.683 satellite DNA lacking Mbol cleavage sites suggests that amplification of sequence variants may have occurred during the evolution of this satellite. While the distribution of satellite DNA appears to be different in being interspersed along the holocentric chromosomes of L. flaccida, the structure and mode of evolution of highly repeated DNA appears to follow the same pattern as seen in organisms with localized centromeres.Key words: Luzula, holocentry, highly repeated DNA.

Download Full-text

Characterization of a highly repeated DNA sequence family in five species of the genus Eulemur

Gene ◽

10.1016/s0378-1119(01)00653-9 ◽

2001 ◽

Vol 275 (2) ◽

pp. 305-310 ◽

Cited By ~ 6

Author(s):

Mario Ventura ◽

Michele Boniotto ◽

Maria Francesca Cardone ◽

Luigi Fulizio ◽

Nicoletta Archidiacono ◽

...

Keyword(s):

Dna Sequence ◽

Sequence Family ◽

Repeated Dna ◽

Highly Repeated Dna ◽

Repeated Dna Sequence

Download Full-text

Variability and evolution of highly repeated DNA sequences in the genus Beta

Genome ◽

10.1139/g93-142 ◽

1993 ◽

Vol 36 (6) ◽

pp. 1074-1079 ◽

Cited By ~ 27

Author(s):

T. Schmidt ◽

J. S. Heslop-Harrison

Keyword(s):

Dna Sequences ◽

Satellite Dna ◽

Polyacrylamide Gel Electrophoresis ◽

Type I ◽

Dna Repeats ◽

Repeated Dna ◽

Copy Numbers ◽

Wild Beet ◽

Lower Variability

Satellite DNA from wild beet species was separated from restriction endonuclease digested genomic DNA by polyacrylamide gel electrophoresis. Two nonhomologous HaeIII satellite DNA repeats were cloned from the wild beet Beta trigyna. The type I repeat is 140–149 bp long and AT rich, while the type II is 162 bp in size and GC rich. A third repetitive HaeIII element cloned from the related wild beet B. corolliflora was shown to be organized as a HinfI satellite DNA family in the cultivated beet B. vulgaris ssp. vulgaris and the wild beet B. vulgaris ssp. maritima. This type III satellite monomer is 149 bp long and contains a high number of short direct subrepeats. The monomer was found in different genomic organizations and copy numbers in all sections of the genus Beta indicating an amplification early in the phylogeny. The HaeIII repeats from B. trigyna are characterized by a lower variability and form long tandem arrays in the genomes of Corollinae species. The investigation of the distribution of all three sequence families provided data that may contribute to the solution of taxonomic problems of the genus Beta and be useful in the characterization of hybrids and derived lines with alien wild beet chromosomes.Key words: satellite DNA, Beta vulgaris, Beta corolliflora, Beta trigyna, evolution, repetitive DNA.

Download Full-text