Somatic embryogenesis in white spruce (Picea glauca): genetic control in somatic embryos exposed to storage, maturation treatments, germination, and cryopreservation

1994 ◽  
Vol 89 (6) ◽  
pp. 742-750 ◽  
Author(s):  
Y. S. Park ◽  
S. E. Pond ◽  
J. M. Bonga
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Control ◽  
Picea Glauca ◽  
Somatic Embryos ◽  
White Spruce

Somatic embryogenesis and plantlet regeneration from embryos isolated from stored seeds of Picea glauca

1990 ◽  
Vol 68 (2) ◽  
pp. 236-242 ◽  
Author(s):  
F. M. Tremblay
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Picea Glauca ◽  
Somatic Embryos ◽  
Germination Rate ◽  
Casein Hydrolysate ◽  
White Spruce ◽  
Zygotic Embryos ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

White spruce (Picea glauca) embryogenic callus was obtained using 3- to 11-year-old seeds as a source of zygotic embryos. They were cultured on half-strength Litvay's medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid, 5 μM benzylaminopurine, 1 g/L casein hydrolysate, 500 mg/L glutamine, and 1% sucrose. The frequency of induction of embryogenic callus was significantly improved by incubation at 25 °C and by a 4-h imbibition of the seeds. The yield of embryogenic callus was significantly affected by the geographic provenance of the seeds and by their number of years in storage. A significant correlation was also found between the yield of embryogénie callus and the percentage of germination of the seedlot used. Even after 11 years of storage, 40% of the zygotic embryos could produce an embryogenic callus when dissected from seeds with a high germination rate. Somatic embryos were matured after transfer onto an embryo development medium composed of the same medium but including 6% sucrose, 1 μM 2,4-dichlorophenoxyacetic acid, and 5 μM kinetin. The somatic embryos developed further under in vitro conditions and were then transplanted into soil. The somatic embryoderived plantlets established in the greenhouse were similar to control plantlets obtained from germinated seeds. Mature embryos from stored seeds were shown to constitute a valuable source for white spruce somatic embryogenesis.

scholarly journals Genetic control and evolutionary potential of a constitutive resistance mechanism against the spruce budworm (Choristoneura fumiferana) in white spruce (Picea glauca)

Heredity ◽  
2018 ◽  
Vol 121 (2) ◽  
pp. 142-154 ◽  
Author(s):  
Claudia Méndez-Espinoza ◽  
Geneviève J. Parent ◽  
Patrick Lenz ◽  
André Rainville ◽  
Laurence Tremblay ◽  
...  
Keyword(s):  
Genetic Control ◽  
Picea Glauca ◽  
Choristoneura Fumiferana ◽  
Resistance Mechanism ◽  
Spruce Budworm ◽  
White Spruce ◽  
Evolutionary Potential ◽  
Constitutive Resistance

Transcript profiles of stress-related genes in developing white spruce (Picea glauca) somatic embryos cultured with polyethylene glycol

Plant Science ◽  
2003 ◽  
Vol 165 (4) ◽  
pp. 719-729 ◽  
Author(s):  
Claudio Stasolla ◽  
Leonel van Zyl ◽  
Ulrika Egertsdotter ◽  
Deborah Craig ◽  
Wenbin Liu ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Picea Glauca ◽  
Somatic Embryos ◽  
White Spruce ◽  
Stress Related Genes ◽  
Transcript Profiles

Induction of Somatic Embryos and Plantlets from Cryopreserved Cell Cultures of White Spruce (Picea glauca)

1988 ◽  
Vol 132 (5) ◽  
pp. 529-539 ◽  
Author(s):  
K.K. Kartha ◽  
L.C. Fowke ◽  
N.L. Leung ◽  
K.L. Caswell ◽  
I. Hakman
Keyword(s):  
Cell Cultures ◽  
Picea Glauca ◽  
Somatic Embryos ◽  
White Spruce ◽  
Cryopreserved Cell

The effectiveness of various nitrogen sources in white spruce [Picea glauca (Moench) Voss] somatic embryogenesis

1997 ◽  
Vol 16 (6) ◽  
pp. 411-415
Author(s):  
J. D. Barrett ◽  
Y. S. Park ◽  
J. M. Bonga
Keyword(s):  
Somatic Embryogenesis ◽  
Picea Glauca ◽  
Nitrogen Sources ◽  
White Spruce

scholarly journals Potential link between biotic defense activation and recalcitrance to induction of somatic embryogenesis in shoot primordia from adult trees of white spruce (Picea glauca)

2013 ◽  
Vol 13 (1) ◽  
pp. 116 ◽  
Author(s):  
Robert G Rutledge ◽  
Don Stewart ◽  
Sébastien Caron ◽  
Cathy Overton ◽  
Bryan Boyle ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Picea Glauca ◽  
White Spruce ◽  
Shoot Primordia ◽  
Biotic Defense ◽  
Adult Trees ◽  
Potential Link
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