Isolation and identification of Triticum aestivum L. em. Thell. cv Chinese Spring-T. peregrinum Hackel disomic chromosome addition lines

1996 ◽  
Vol 92 (5) ◽  
pp. 591-598 ◽  
Author(s):  
Y. -C. Yang ◽  
N. A. Tuleen ◽  
G. E. Hart
Keyword(s):  
Triticum Aestivum ◽  
Chinese Spring ◽  
Triticum Aestivum L ◽  
Addition Lines ◽  
Isolation And Identification ◽  
Chromosome Addition ◽  
Chromosome Addition Lines

The addition of Aegilops variabilis chromosomes to Triticum aestivum and their identification

1983 ◽  
Vol 25 (1) ◽  
pp. 76-84 ◽  
Author(s):  
D. C. Jewell ◽  
C. J. Driscoll
Keyword(s):  
Triticum Aestivum ◽  
Triticum Aestivum L ◽  
Addition Lines ◽  
Single Chromosome ◽  
Aegilops Umbellulata ◽  
Chromosome Addition ◽  
Aegilops Longissima ◽  
Chromosome Addition Lines ◽  
Aegilops Variabilis ◽  
The Relationship

Nine of the 14 possible single chromosome addition lines of the tetraploid species Aegilops variabilis Eig. (CuCuSvSv) to Triticum aestivum L. cv. Chinese Spring (AA BB DD) have been isolated and identified. The nine Aegilops variabilis addition lines were compared with the available addition lines of Aegilops umbellulata (CuCu) and Aegilops longissima (SvSv) to further elucidate the relationship between these two diploids and the tetraploid Aegilops variabilis. Differences were observed between the same chromosomes isolated from the diploid and the tetraploid and discussed. After taking into account banding pattern polymorphisms, Aegilops umbellulata was confirmed as the donor of the Cu genome, and evidence indicated that Aegilops longissima probably is the donor of the other genome (Sv) in the tetraploid Aegilops variabilis.

scholarly journals The genetic control of triosephosphate isomerase of hexaploid wheat and other Triticeae species

1985 ◽  
Vol 45 (2) ◽  
pp. 127-142 ◽  
Author(s):  
Michael E. Pietro ◽  
Gary E. Hart
Keyword(s):  
Triticum Aestivum ◽  
Hordeum Vulgare ◽  
Secale Cereale ◽  
Hexaploid Wheat ◽  
Chinese Spring ◽  
Triosephosphate Isomerase ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Derivatives Of

SummaryThe zymogram phenotypes of triosephosphate isomerase (TPI) were determined for a large number of aneuploid derivatives of Triticum aestivum cv. ‘Chinese Spring’ and for six wheat-alien species chromosome addition series. Examination of the available compensating nullisomic-tetrasomic and homoeologous groups 3 and 5 ditelosomic lines of Chinese Spring disclosed that T. aestivum possesses two systems of dimeric TPI isozymes, designated TPI-1 and TPI-2. The genes TPI-A1, TPI-B1 and TPI-D1 were located in Chinese Spring chromosome arms 3Ap, 3Bp and 3Dp, respectively and the genes TPI-A2, TPI-B2 and TPI-D2 in chromosome arms 5Aq, 5Bq and 5Dq, respectively. TPI-1 genes were also located in Hordeum vulgare cv. Betzes chromosome 3H, T. longissimum chromosome G, Elytrigia elongata chromosome 3E, and Secale cereale cvs. Imperial and Dakold chromosome 3R. TPI-2 genes were found in Betzes chromosome 5H, T. umbellulatum chromosome 5U, T. longissimum chromosome F, and Imperial and Dakold chromosome 5R. These gene locations provide evidence of homoeology between the alien chromosomes in which the genes are located and the chromosomes of homoeologous groups 3 and 5 of Chinese Spring, respectively. Evidence was obtained for the presence of a TPI-R2 gene in each of the T. aestivum cv. Kharkov -S. cereale cv. Dakold chromosome addition lines studied suggesting that this gene is present in the wheat genome in each member of this addition series.

Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 959-970 ◽  
Author(s):  
I G Adonina ◽  
E A Salina ◽  
E G Pestsova ◽  
M S Röder
Keyword(s):  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Aegilops Longissima ◽  
Chromosome Addition Lines

Overall, 253 genomic wheat (Triticum aestivum) microsatellite markers were studied for their transferability to the diploid species Aegilops speltoides, Aegilops longissima, and Aegilops searsii, representing the S genome. In total, 88% of all the analyzed primer pairs of markers derived from the B genome of hexaploid wheat amplified DNA fragments in the genomes of the studied species. The transferability of simple sequence repeat (SSR) markers of the T. aestivum A and D genomes totaled 74%. Triticum aestivum – Ae. speltoides, T. aestivum – Ae. longissima, and T. aestivum – Ae. searsii chromosome addition lines allowed us to determine the chromosomal localizations of 103 microsatellite markers in the Aegilops genomes. The majority of them were localized to homoeologous chromosomes in the genome of Aegilops. Several instances of nonhomoeologous localization of T. aestivum SSR markers in the Aegilops genome were considered to be either amplification of other loci or putative translocations. The results of microsatellite analysis were used to study phylogenetic relationships among the 3 species of the Sitopsis section (Ae. speltoides, Ae. longissima, and Ae. searsii) and T. aestivum. The dendrogram obtained generally reflects the current views on phylogenetic relationships among these species.Key words: Triticum aestivum, Aegilops speltoides, Aegilops longissima, Aegilops searsii, microsatellite, SSR, chromosome addition lines, phylogeny.

Cytogenetic identification of Triticum peregrinum chromosomes added to common wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 272-276 ◽  
Author(s):  
B. Friebe ◽  
E. D. Badaeva ◽  
B. S. Gill ◽  
N. A. Tuleen
Keyword(s):  
Triticum Aestivum ◽  
Key Words ◽  
Common Wheat ◽  
Addition Lines ◽  
Karyotypic Analysis ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Donor Species

C-banded karyotypes of a complete set of 14 Triticum peregrinum whole chromosome addition lines and 25 telosomic addition lines are reported. The added T. peregrinum chromosomes were not structurally rearranged compared with the corresponding chromosomes of the donor accession. Comprehensive karyotypic analysis confirmed Triticum umbellulatum as the donor species of the Uv genome and identified Triticum longissimum as the donor species of the Sv genome of T. peregrinum. Neither the Uv nor Sv genome chromosomes of the T. peregrinum accession showed large modifications when compared with the ancestral U and S1 genomes. Key words : Triticum aestivum, Triticum peregrinum, Triticum umbellulatum, Triticum longissimum, chromosome addition lines, C-banding.

scholarly journals Use of isozymes as chromosome markers in the isolation and characterization of wheat-barley chromosome addition lines

1980 ◽  
Vol 36 (3) ◽  
pp. 311-325 ◽  
Author(s):  
Gary E. Hart ◽  
A. K. M. R. Islam ◽  
K. W. Shepherd
Keyword(s):  
Chinese Spring ◽  
Chromosome 1 ◽  
Addition Lines ◽  
Chromosome 4 ◽  
Glutamic Oxaloacetic Transaminase ◽  
Chromosome Addition ◽  
Isolation And Characterization ◽  
Chinese Spring Wheat ◽  
Chromosome Addition Lines

SUMMARYThe alcohol dehydrogenase (ADH), glutamic oxaloacetic transaminase (GOT), aminopeptidase (AMP), endopeptidase (EP), and esterase (EST) zymogram phenotypes of Chinese Spring wheat, Betzes barley, Chinese Spring-Betzes heptaploids, and a number of presumptive Betzes chromosome additions to Chinese Spring were determined. It was found that four disomic chromosome addition lines could be distinguished from one another and from the other three possible lines on the basis of the zymogram phenotypes of these isozymes.The structural gene Adh-H1 was located in Betzes chromosome 4, the genes Got-H2 and Amp-H1 in chromosome 6, and the gene Ep-H1 in chromosome 1. These gene locations provide evidence of homoeology between Betzes chromosomes 4, 6, and 1 and the Chinese Spring chromosomes of homoeologous groups 4, 6, and 7, respectively.

Development of a complete set of Triticum aestivum-Aegilops speltoides chromosome addition lines

2000 ◽  
Vol 101 (1-2) ◽  
pp. 51-58 ◽  
Author(s):  
B. Friebe ◽  
L. L. Qi ◽  
S. Nasuda ◽  
P. Zhang ◽  
N. A. Tuleen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Speltoides Chromosome

Isolation and characterization of wheat–Elytrigia elongata chromosome 3E and 5E addition and substitution lines

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 519-524 ◽  
Author(s):  
N. A. Tuleen ◽  
G. E. Hart
Keyword(s):  
Chinese Spring ◽  
Addition Lines ◽  
Homoeologous Group ◽  
Substitution Lines ◽  
Agropyron Elongatum ◽  
Chromosome Addition ◽  
Isolation And Characterization ◽  
Chromosome Addition Lines ◽  
Group 3 ◽  
Group 5

Isozyme markers were used to develop Triticum aestivum cv. Chinese Spring–Elytrigia elongata (= Agropyron elongatum, 2n = 14, genome E) disomic 3E and 5E addition lines. Subsequently, all possible lines containing 3E and 5E substituted for wheat homoeologues and several 3E and 5E ditelosomic addition and substitution lines were developed. Plants containing chromosome 3E substituted for wheat chromosomes of homoeologous group 3 are similar to 'Chinese Spring' in vigor and fertility while plants containing 3EL substituted for chromosomes of group 3 are less fertile than 'Chinese Spring'. This indicates that both arms of 3E are involved in sporophytic compensation. Plants containing chromosome 5E substituted for wheat chromosomes of homoeologous group 5 are as vigorous but less fertile than 'Chinese Spring'. 5EL (5A) and 5EL (5B) plants are lower in fertility than 5E (5A) and 5E (5B) plants, indicating that both arms of 5E are involved in sporophytic compensation. 5E (5D) and 5EL (5D) plants are similar in fertility. Male gametophytes in which 3E or 5E replaces a wheat homoeologue function at a lower rate than normal gametes.Key words: wheat, Triticum, Elytrigia elongata, alien chromosome addition lines.

scholarly journals Chromosomal locations of eleven Elytrigia elongata (= Agropyron elongatum) isozyme structural genes

1983 ◽  
Vol 41 (2) ◽  
pp. 181-202 ◽  
Author(s):  
Gary E. Hart ◽  
Neal A. Tuleen
Keyword(s):  
Chinese Spring ◽  
Common Ancestor ◽  
Addition Lines ◽  
Structural Genes ◽  
Agropyron Elongatum ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Study Support ◽  
Disomic Addition Lines ◽  
Valuable Role

SUMMARYThe zymogram phenotypes of 11 enzymes were determined for 22 Triticum aestivum cv. Chinese Spring-Elytrigia elongata disomic and ditelosomic chromosome addition lines. Eleven isozyme structural genes were located in specific arms of six E. elongata chromosomes, as follows: Gpi-E1 in 1ES, Est-E1 in 3ES, Got-E3 in 3EL, Adh-E1 and Lpx-E1 in 4ES, Adh-E2 and Lpx-E2 in 5EL, Amp-E1 in 6Eα, Adh-E3 and Got-E2 in 6Eβ, and Ep-E1 in 7EL. The E. elongata chromosomes present in five disomic addition lines have previously been designated 1E, 2E, 4E, 6E, and 7E to indicate their homoeology with Chinese Spring chromosomes. The results of this study support these designations. The development of disomic putative 3E and 5E addition lines is reported. The added chromosomes designated IV, V, and VI that are present in three of the seven original disomic T. aestivum-E. elongata addition lines are translocated. Evidence that VL and VIL are opposite arms of 2E and that IV is partially homoeologous to 3E has been published. The results reported in this paper indicate that IVS = 3ES, IVL = 7EL, VS = 3ES, and VIS = 5ES and are consistent with VL and VIL being opposite arms of 2E. The synteny relationships of the 11 E. elongata isozyme genes identified in this study are fully consistent with those of homoeologous T. aestivum cv. Chinese Spring genes and thus provide evidence that the gene synteny groups which these two species inherited from their common ancestor are conserved. This study further documents the valuable role that studies of isozyme genes can play in the isolation, characterization, and maintenance of alien chromosomes, telosomes, and chromosomal segments in wheat strains.

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