Production and analysis of asymmetric hybrid plants between monocotyledon (Oryza sativa L.) and dicotyledon (Daucus carota L.)

1994 ◽  
Vol 89-89 (2-3) ◽  
pp. 365-371 ◽  
Author(s):  
H. Kisaka ◽  
H. Lee ◽  
M. Kisaka ◽  
A. Kanno ◽  
K. Kang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Daucus Carota ◽  
Oryza Sativa L ◽  
Daucus Carota L ◽  
Hybrid Plants ◽  
Asymmetric Hybrid ◽  
Asymmetric Hybrid Plants

scholarly journals Characterization of Progenies from Intergeneric Hybridization Between Oryza sativa L. and Porteresia coarctata (Roxb.) Tateoka

2017 ◽  
Vol 27 (1) ◽  
pp. 63-76
Author(s):  
Tasmia Islam ◽  
Sudip Biswas ◽  
Umme Habiba Mita ◽  
RH Sarker ◽  
M Sazzadur Rahman ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Ssr Markers ◽  
Oryza Sativa L ◽  
Intergeneric Hybridization ◽  
Chromosome Loss ◽  
Salt Tolerant ◽  
Porteresia Coarctata ◽  
Maternal Parent ◽  
Hybrid Plants ◽  
Coastal Belt

Porteresia coarctata (Roxb.) Tateoka is an endemic halophyte growing all over the coastal belt of Bangladesh, propagating through rhizomes and setting a few ricelike grains. So exploiting the genetic potential of this wild rice as salt tolerant donor in possible wide crosses with rice (2n = 24) could be useful. We attempted intergeneric hybridization between Oryza sativa L. and P. coarctata. The survival rate of hybrid progenies in embryo culture was low but among them 2 hybrid plants were successfully matured from the intergeneric cross between the cultivated induced tetraploid of rice, Latisail (2n = 4x = 48) and P. coarctata (2n = 48). The hybrid plants could be successfully established in soil and were not like either of the parents in morphology although some of their features were similar to their maternal parent, Latisail (4x). Both of the hybrids were investigated through physiological analysis under salinity stress and molecular analyses with rice specific SSR markers. Molecular analysis of the F1 DNA with only 3 SSR markers, RM581, RM20224 and RM25271, out of 36 others tested, showed bands specific to both of the parents, while all had common bands with the maternal parent. Dendrogram analysis of the hybrids with the 36 SSR markers, show that P. coarctata forms a different clade and is clearly separated from Latisail and the hybrids. The putative hybrids however made a subgroup with Latisail. These observations could be possibly explained if chromosome loss of the paternal parent had occurred or may be it was a pleotropic effect of intergeneric hybridization. Physiological screening of the hybrid progenies at the F2 generation in seedling stage showed better result in leaf damage score (LDS) and salinity tolerance than their maternal parent Latisail (4x) at 150 mM salt stress for 10 days. F2 plants from one of the hybrid plants (H‐2) showed better performance but there was a large variation in response from each of the individual progenies. So, it is likely that some of the salt tolerant characteristics of the pollen parent might have been transferred to the recipient Latisail (4x). For introgression of better salt tolerant loci from P. coarctata, more wide hybrids will need to be produced and repeatedly crossed with P. coarctata.Plant Tissue Cult. & Biotech. 27(1): 63-76, 2017 (June)

scholarly journals Nuclear Genome Organization of Asymmetric Intergeneric Somatic Hybrid Plants between Lycopersicon esculentum × L. pennellii and Solanum melongena

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 840B-840
Author(s):  
Vladimir M. Samoylov ◽  
Kenneth C. Sink
Keyword(s):  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Blot Hybridization ◽  
Solanum Melongena ◽  
Nuclear Genome ◽  
Nptii Gene ◽  
Dot Blot ◽  
Hybrid Plants ◽  
Asymmetric Hybrid ◽  
Asymmetric Hybrid Plants

Asymmetric somatic hybrids were obtained by PEG/DMSO fusion of protoplasts of a kanamycin-resistant (KmR+) interspecific tomato hybrid L. esculentum × L. pennellii with protoplasts of S. melongena eggplant. Elimination of tomato chromosomes was directed by application of 100, 250, 500, 750, and 1000 Gy of γ-rays to the donor protoplasts. The hybrid nature of selected KmR+ calli was confirmed by RAPDs, PCR amplification of the NptII gene, and Southern hybridization. Flow cytometry revealed that asymmetric hybrid plants were regenerated only from selected somatic hybrid calli that had a ploidy level close to 4n. The amount of donor DNA in three somatic hybrid plants was quantified by dot-blot hybridization with tomato species-specific probes, and was found to be 5% to 7%. Therefore, presence of four to five tomato chromosomes in asymmetric hybrid plants has been calculated. Detection of tomato-specific chromosomes in hybrid plants by RFLP analysis will be presented.

DAYA TERIMA JAJANAN LOKAL BARONCONG DENGAN PENAMBAHAN WORTEL (DAUCUS CAROTA L)

2019 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Suriani Rauf ◽  
Manjilala Manjilala ◽  
Elvi Kusuma
Keyword(s):  
Experimental Study ◽  
Daucus Carota ◽  
Vegetable Consumption ◽  
Negative Impact ◽  
Local Foods ◽  
Ministry Of Health ◽  
Daucus Carota L

The behavior of people who less consume vegetables and fruit can have a negative impact on health. The lack of appetite for vegetable consumption by the society made the researchers interested in making a combination of vegetables, namely carrots with local foods, namely Baroncong.This research aims to determine the acceptability of local baroncong snacks with the addition of carrots.This research is a pre-experimental study. The panelists from this study consisted of 30 Nutrition Department Students from Health Polytechnic Ministry of Health Makassar. Assessment based on taste aspects, color of aroma and texture of baroncong products with the addition of carrots 50%, 75%, 100% with the criteria of very like, like, dislike and very dislike.The results showed that the acceptability of baroncong with the addition of carrots in terms of taste was a concentration of 75%, which was very much as much as 46.7% and liked as much as 50%, the color aspect was 50% concentration consisting of 36.7% likes and likes as much as 53.3%, the aroma aspectis a concentration of 50% which consists of very much as much as 33.3% and likes as much as 63.3%, in terms of aspects of texture are concentrations of 50% and 75%, each of which consists of as much as 23.3% and likes as much as 63, 3%. Based on these data, Baroncong with the addition of carrots received is a concentration of 75%.

Keragaan Morfologi dan Kandungan Antosianin Padi Beras Merah (Oryza sativa L.) di Kecamatan Munte dan Kecamatan Payung, Kabupaten Karo

1970 ◽  
Vol 5 (3) ◽  
pp. 391-403
Author(s):  
Wiwik Winarti ◽  
Eva Sartini Bayu ◽  
Revandy Iskandar Damanik
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
International Rice Research Institute ◽  
Research Institute

Keragaan morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.) pada Kecamatan Munte dan Kecamatan Payung di Kabupaten Karo. Penelitian ini bertujuan untuk mendapatkan informasi morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.). Penelitian ini dimulai dari Juni 2017dan selesai pada Januari 2018 di kecamatan Munte dan Kecamatan Payung Kabupaten Karo.Metode survei deskriptif menggunakan panduan International Rice Research Institute (IRRI). Teknik penentuan lokasi secara sengaja dan pengamblan sampel secara kebetulan.Uji kandungan antosianin menggunakan metode analitik dengan menghomogenkan sampel. Hasil eksplorasi didapatkan 72 genotipe yang dibagi menjadi tiga lokasi lahan. Berdasaran uji kandungan antosianin didapatkan lahan A memiliki kandungan tertinggi yaitu 0,5 mg/100 g dan terendah yaitu lahan B 0,08 mg/100 g.

Efficacy of some insecticide modules against major insect pests and spider population of rice, Oryza sativa L.

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 257-262
Author(s):  
Atanu Seni ◽  
Bhimasen Naik
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Untreated Control ◽  
Insect Pests ◽  
Oryza Sativa L ◽  
Gall Midge ◽  
Stem Borer ◽  
The Other ◽  
The Third ◽  
Treated Plot

Experiments were carried out to assess some insecticide modules against major insect pests of rice. Each module consists of a basal application of carbofuran 3G @ 1 kg a.i ha-1 at 20 DAT and Rynaxypyr 20 SC @ 30 g a.i ha-1 at 45 DAT except untreated control. All modules differ with each other only in third treatment which was applied in 65 DAT. The third treatment includes: Imidacloprid 17.8 SL @ 27 g a.i ha-1, Pymetrozine 50 WG @ 150 g a.i ha-1, Triflumezopyrim 106 SC @ 27 g a.i ha-1, Buprofezin 25 SC @ 250 g a.i ha-1; Glamore (Imidacloprid 40+Ethiprole 40% w/w) 80 WG @ 100 g a.i. ha-1, Thiacloprid 24 SC @ 60 g a.i ha-1, Azadirachtin 0.03 EC @ 8 g a.i ha-1, Dinotefuran 20 SG@ 40 g a.i ha-1 and untreated control. All the treated plots recorded significantly lower percent of dead heart, white ear- head caused by stem borer and silver shoot caused by gall midge. Module with Pymetrozine 50 WG @ 150 g a.i ha-1 treated plot recorded significantly higher per cent reduction of plant hoppers (>80% over untreated control) and produced higher grain yield (50.75 qha-1) than the other modules. Among the different treated modules the maximum number of spiders was found in Azadirachtin 0.03 EC @ 8 g a.i ha-1 treated module plot followed by other treatments.

Correlation Studies on Grain Yield and its Component Traits in Rice (Oryza sativa L.)

2012 ◽  
Vol 2 (11) ◽  
pp. 13-14
Author(s):  
R. ARULMOZHI R. ARULMOZHI ◽  
◽  
Dr. A. MUTHUSWAMY Dr. A. MUTHUSWAMY
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Correlation Studies ◽  
Component Traits

Aplikasi Abu Sekam pada Padi Gogo (Oryza sativa L.) terhadap Kandungan Silikat dan Prolin Daun serta Amilosa dan Protein Biji

2011 ◽  
Vol 16 (1) ◽  
Author(s):  
Ahadiyat Yugi Rahayu ◽  
Tri Harjoso
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Screen House

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh aplikasi abu sekam terhadap kandungan prolin dan silikat daun serta kualitas hasil yaitu protein dan amilosa biji padi gogo dan hubungan korelasi antar keempat komponen tersebut pada kondisi pertanaman 80 persen kapasitas lapang pada skala pot. Penelitian dilakukan di polibag dalam screen house Fakultas Pertanian Unsoed dengan menggunakan rancangan acak kelompok pola faktorial dengan faktor varietas (Situ patenggang, Limboto, Towuti, Batutegi dan Aek sibundong) dan faktor abu sekam (0, 2, 4, 6 t/ha), diulang tiga kali. Hasil penelitian menunjukan bahwa pemberian abu sekam dosis 2−6 t/ha mampu meningkatkan kandungan silikat daun antara 18,49−29,43% dan menurunkan kandungan amilosa biji pada lima varietas sekitar 4,19−6,92%. Pemberian abu sekam dosis 2−6 t/ha mampu meningkatkan kandungan prolin daun antara 27,56−70,63% dan protein biji antara 2,35−16,71%. Antarvarietas menunjukan bahwa kandungan prolin tertinggi dihasilkan oleh varietas Batu tegi 18,58 persen dan protein biji pada varietas Situ patenggang 9,55%. Terdapat korelasi antar karakter fisiologis yaitu antara silikat-prolin (0,62) dan kandungan protein-amilosa biji (-0,78).

Produksi dan Mutu Benih Padi (Oryza sativa L.) Ratun dengan Aplikasi Bakteri Synechococcus sp. pada Berbagai Salinitas Media

2017 ◽  
Author(s):  
Agung Sugiharto ◽  
Dwi Rahmawati ◽  
FNU Prayitno
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Synechococcus Sp ◽  
Range Test

Salah satu upaya untuk meningkatkan produksi dan mutu benih di lahan salin yaitu melalui penggunaan metode ratun dengan penambahan bakteri sintetik Synechoccocus sp. Penelitian ini bertujuan untuk mengetahui produksi dan mutu benih padi ratun yang tercekam salinitas dengan penambahan bakteri sintetik Synechoccocus sp. Penelitian dilaksanakan pada bulan Juli - Desember 2015 di Desa Suco, Kecamatan Mumbul Sari Jember dan Laboratorium Teknologi Benih Politeknik Negeri Jember. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok Faktorial (RAK) dengan 2 faktor dan 3 ulangan. Faktor pertama adalah cekaman Salinitas (S) yang terdiri dari 5 taraf, S1 = tanpa cekaman (kontrol), S2 = cekaman salinitas 1000 ppm, S3 = cekaman salinitas 2000 ppm, S4 = cekaman salinitas 3000 ppm, S5 = cekaman salinitas 4000 ppm. Faktor kedua inokulasi bakteri sintetik Synechoccocus sp. (B), yang terdiri dari 2 taraf, B1 = tanpa inokulasi bakteri (kontrol), B2 = Inokulasi sintetik Synechoccocus sp. Data dianalisis menggunakan uji F (ANOVA) dan dilanjutkan dengan perhitungan Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukan perlakuan salinitas 1000 ppm (S2) menghasilkan jumlah anakan ratun produktif tertinggi yaitu 19,33 anakan. Cl- mempunyai fungsi utama dalam reaksi fotosintesis sehingga cekaman salintas pada perlakuan 1000 ppm (S2) dapat ditoleran oleh tanaman padi varietas Ciherang pada fase vegetatif. Pemberian Bakteri (B) Synechococcus sp. mampu menghasilkan tunas ratun tertinggi pada fase vegetatif yaitu 40,10 cm. Interaksi dari dua perlakuan menunjukan hasil yang nyata pada parameter jumlah gabah bernas yaitu cekaman salinitas 4000 ppm dengan inokulasi bakteri (B2S5) menghasilkan gabah bernas yang paling tinggi sebesar 99,06 butir. Inokulasi tanaman dengan Synechococcus sp. mampu meningkatkan kandungan nitrogen dan kandungan klorofil dalam jaringan tanaman. Interaksi antara cekaman salinitas 1000 ppm dengan inokulasi bakteri (B2S2) menghasilkan produksi per Ha yang paling tinggi yaitu 1,389 ton dan potensi produksi per Ha tertinggi yaitu 1.66 ton/ha.

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