Characterization by photoaffinity labeling of a steroid binding protein in rat liver plasma membrane

1992 ◽  
Vol 125 (2) ◽  
Author(s):  
I�aki Ibarrola ◽  
Ana Alejandro ◽  
Aida Marino ◽  
Mar�aJ. Sancho ◽  
Jos�M. Macarulla ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Binding Protein ◽  
Photoaffinity Labeling ◽  
Liver Plasma Membrane ◽  
Steroid Binding ◽  
Steroid Binding Protein

Photoaffinity Labeling of a Neuroactive Steroid Binding Protein in Rat Brain

2002 ◽  
Vol 96 (Sup 2) ◽  
pp. A814
Author(s):  
Ramin Darbandi-Tonkabon ◽  
Randy Hastings ◽  
Chun-Min Zeng ◽  
Douglas F. Covey ◽  
Alex S. Evers
Keyword(s):  
Rat Brain ◽  
Binding Protein ◽  
Photoaffinity Labeling ◽  
Neuroactive Steroid ◽  
Steroid Binding ◽  
Steroid Binding Protein

Plasma membrane proteome analysis identifies a role of barley membrane steroid binding protein in root architecture response to salinity

2018 ◽  
Vol 41 (6) ◽  
pp. 1311-1330 ◽  
Author(s):  
Katja Witzel ◽  
Andrea Matros ◽  
Anders L.B. Møller ◽  
Eswarayya Ramireddy ◽  
Christine Finnie ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Root Architecture ◽  
Binding Protein ◽  
Proteome Analysis ◽  
Membrane Proteome ◽  
Steroid Binding ◽  
Steroid Binding Protein

An Unusual Sex Steroid-Binding Protein in Mature Male Rat Liver Cytosol*

Endocrinology ◽  
1978 ◽  
Vol 103 (5) ◽  
pp. 1636-1646 ◽  
Author(s):  
ROBERT B. DICKSON ◽  
RAYMOND F. ATEN ◽  
ARNOLD J. EISENFELD
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Mature Male ◽  
Sex Steroid ◽  
Male Rat ◽  
Liver Cytosol ◽  
Steroid Binding ◽  
Rat Liver Cytosol ◽  
Steroid Binding Protein

Foetal steroid binding protein in British and Japanese women

1987 ◽  
Vol 114 (4) ◽  
pp. 584-588 ◽  
Author(s):  
M. Jawed Iqbal ◽  
Alastair Forbes ◽  
Mark L. Wilkinson ◽  
John W. Moore ◽  
Roger Williams ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Binding Protein ◽  
Premenopausal Women ◽  
Japanese Women ◽  
Sex Hormone Binding Globulin ◽  
Partial Purification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Characteristics ◽  
Steroid Binding ◽  
Steroid Binding Protein

Abstract. In order to examine the newly-discovered sex-steroid binding protein, foetal steroid binding protein (FSBP) in different populations, its binding characteristics and its level were studied by two-tier column ligand binding assay and enzyme-linked immunosorbent assay (ELISA) respectively. In 10 Japanese premenopausal women, analysis of 5α-dihydrotestosterone (DHT) binding in the Cibacron Blue 3GA-Sepharose 6B portion of the column showed a rising plateau pattern with a mean maximum binding of 31.1 ± 7.41%, whereas of 9 similar British women, 8 displayed unsaturable, non-cooperative binding of 11.6 ± 8.22% (P < 0.01). After partial purification of FSBP in these samples, the protein exhibited saturable binding kinetics, median binding 25 (interquartiles 23–34) and 19 (13–25) nmol DHT/l in Japanese and British women, respectively (P < 0.05). By analyzing FSBP by ELISA in 56 Japanese (45 premenopausal) and 59 British (25 premenopausal) women, higher levels were obtained in the whole Japanese group (P = 0.0016) and in the premenopausal Japanese women (P = 0.018) than in their British counterparts. In both nationalities, FSBP levels were higher in premenopausal women, and there was a significant negative correlation of FSBP with age in both populations, particularly in postmenopausal women. FSBP levels did not correlate with weight, parity, sex hormone binding globulin or albumin levels. The influence of FSBP on free steroid levels remains unclear, but some relationship with ovarian function seems a possibility.

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