The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D

1987 ◽  
Vol 75 (1) ◽  
pp. 117-126 ◽  
Author(s):  
N. G. Halford ◽  
J. Forde ◽  
O. D. Anderson ◽  
F. C. Greene ◽  
P. R. Shewry
Keyword(s):  
Triticum Aestivum ◽  
Amino Acid ◽  
Bread Wheat ◽  
Triticum Aestivum L ◽  
Amino Acid Sequences ◽  
Glutenin Subunit ◽  
Subunit Gene ◽  
Hmw Glutenin Subunit ◽  
Hmw Glutenin

scholarly journals Conformational differences between two wheat (Triticum aestivum) ‘high-molecular-weight’ glutenin subunits are due to a short region containing six amino acid differences

1989 ◽  
Vol 263 (3) ◽  
pp. 837-842 ◽  
Author(s):  
A P Goldsbrough ◽  
N J Bulleid ◽  
R B Freedman ◽  
R B Flavell
Keyword(s):  
Molecular Weight ◽  
Triticum Aestivum ◽  
Amino Acid ◽  
High Molecular Weight ◽  
Polyacrylamide Gel Electrophoresis ◽  
Amino Acid Sequences ◽  
Glutenin Subunits ◽  
Sds Page ◽  
Hmw Glutenin

‘High-molecular-weight’ (HMW, high-Mr) glutenin subunits are protein constituents of wheat (Triticum aestivum) seeds and are responsible in part for the viscoelasticity of the dough used to make bread. Two subunits, numbered 10 and 12, are the products of allelic genes. Their amino acid sequences have been derived from the nucleic acid sequences of the respective genes. Subunit 10 has fewer amino acids than subunit 12, but migrates more slowly on SDS/PAGE (polyacrylamide-gel electrophoresis). This anomaly is due to between one and six of the amino acid differences between the subunits, localized towards the C-terminal end of the proteins. This has been established by making chimaeric genes between the genes for subunits 10 and 12, transcribing and translating them in vitro and analysing the products by SDS/PAGE. The postulated conformational differences between subunits 10 and 12 are discussed in relation to current hypotheses for the structure of HMW glutenin subunits.

Molecular cloning of a novel chimeric HMW glutenin subunit gene 1Dx5′ from a common wheat line W958

2010 ◽  
Vol 28 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Bo Feng ◽  
Xueli An ◽  
Zhibin Xu ◽  
Dongcheng Liu ◽  
Aiming Zhang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Common Wheat ◽  
Glutenin Subunit ◽  
Wheat Line ◽  
Subunit Gene ◽  
Hmw Glutenin Subunit ◽  
Hmw Glutenin
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