Cell differentiation of alveolar epithelium in the developing rat lung: ultrahistochemical studies of glycoconjugates on the epithelial cell surface

1993 ◽  
Vol 100 (5) ◽  
pp. 331-340 ◽  
Author(s):  
Hirohiko Iwatsuki ◽  
Kazunobu Sasaki ◽  
Masumi Suda ◽  
Chikako Itano
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cell ◽  
Cell Surface ◽  
Alveolar Epithelium ◽  
Rat Lung ◽  
Epithelial Cell Surface ◽  
Developing Rat

scholarly journals A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity

2007 ◽  
Vol 178 (2) ◽  
pp. 323-335 ◽  
Author(s):  
Lene N. Nejsum ◽  
W. James Nelson
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Cell Surface ◽  
Basolateral Membrane ◽  
Surface Polarity ◽  
Cell Contacts ◽  
Protein Receptors ◽  
Epithelial Cell Surface ◽  
E Cadherin ◽  
Cell Cell

Mechanisms involved in maintaining plasma membrane domains in fully polarized epithelial cells are known, but when and how directed protein sorting and trafficking occur to initiate cell surface polarity are not. We tested whether establishment of the basolateral membrane domain and E-cadherin–mediated epithelial cell–cell adhesion are mechanistically linked. We show that the basolateral membrane aquaporin (AQP)-3, but not the equivalent apical membrane AQP5, is delivered in post-Golgi structures directly to forming cell–cell contacts where it co-accumulates precisely with E-cadherin. Functional disruption of individual components of a putative lateral targeting patch (e.g., microtubules, the exocyst, and soluble N-ethylmaleimide–sensitive factor attachment protein receptors) did not inhibit cell–cell adhesion or colocalization of the other components with E-cadherin, but each blocked AQP3 delivery to forming cell–cell contacts. Thus, components of the lateral targeting patch localize independently of each other to cell–cell contacts but collectively function as a holocomplex to specify basolateral vesicle delivery to nascent cell–cell contacts and immediately initiate cell surface polarity.

Ubiquitin Ligase MARCH8 attenuates Graft versus Host Disease via Regulation of Gut Epithelial Cell Surface MHC II Expression.

2018 ◽  
Vol 102 ◽  
pp. S300
Author(s):  
Katie E Lineburg ◽  
Laetitia Le Texier ◽  
Michelle Melino ◽  
Ran Wang ◽  
Andrew Clouston ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Ubiquitin Ligase ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host ◽  
Mhc Ii ◽  
Epithelial Cell Surface

Simultaneous Fluorescent Identification of Odontoblasts and Ameloblasts

2020 ◽  
pp. 002203452097457
Author(s):  
K. Isono ◽  
E. Takahashi ◽  
I. Miyoshi ◽  
M. Tsuneto ◽  
M. Hikosaka-Kuniishi ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Tooth Development ◽  
Cell Surface Molecules ◽  
Male Mice ◽  
Mineral Density ◽  
Surface Molecules ◽  
Epithelial Cell Surface ◽  
Dentin Matrix ◽  
Cell Fractions

The tooth is mainly composed of dentin and enamel. Identification of dentin-producing odontoblasts and enamel-producing ameloblasts using reporter techniques is useful to study tooth development and regeneration with tissue engineering. Ameloblasts express Amelogenin, Ameloblastin, Enamelin, and Amelotin, whereas odontoblasts express Dentin sialophosphoprotein ( Dspp) and Dentin matrix protein1 ( Dmp1). Although there are several transgenic lines using promoter elements or bacterial artificial chromosomes (BACs) to label odontoblasts and ameloblasts, there is a possibility that the expression patterns vary from the endogenous genes. Here, we established 2 lines of mice where tdTomato was knocked into the second exon of X-chromosomal Amelogenin ( Amelx), and green fluorescent protein (GFP) was knocked into the second exon of Dspp. tdTomato and GFP were highly expressed on secretory ameloblasts and secretory and fully differentiated odontoblasts, respectively. In addition, DSPP and AMELX were not produced in the dentin matrix and enamel matrix of Dspp GFP/GFP and Amelx tdTomato male mice (as representative of AmelxtdTomato/Y hemizygous male mice), respectively. Moreover, micro–computed tomography analysis of Amelx tdTomato male mice revealed a notable reduction in enamel volume but increased dentin mineral density. Dspp GFP/GFP mice had reduced dentin mineral density. To identify odontoblasts and ameloblasts from developing tooth, we examined the expression of mesenchymal cell surface molecules CD90, CD166 and epithelial cell surface molecules CD49f, Epcam1 with fluorescence on odontoblasts and ameloblasts in these mice. We found that GFP+ odontoblasts and tdTomato+ ameloblasts in tooth germ from 0.5-d-old DsppGFP/+ mice and Amelx tdTomato male mice were enriched in CD45−/Ter119−/Epcam1−/CD90+/Integrin α4+cell fractions and CD45−/Ter119−/Epcam1+/CD49f+/CD147+ cell fractions, respectively. By using antibodies against mesenchymal and epithelial cell surface molecules and fluorescence, we can easily distinguish odontoblasts from ameloblasts and isolate each cell for further studies. These mice would serve as useful models for tooth development and regeneration as well as provide concurrent observation for the differentiation processes of odontoblasts and ameloblasts in vivo and in vitro.

scholarly journals Phenotypic Variation in Molecular Mimicry betweenHelicobacter pyloriLipopolysaccharides and Human Gastric Epithelial Cell Surface Glycoforms

2001 ◽  
Vol 277 (8) ◽  
pp. 5785-5795 ◽  
Author(s):  
Anthony P. Moran ◽  
Yuriy A. Knirel ◽  
Sof'ya N. Senchenkova ◽  
Göran Widmalm ◽  
Sean O. Hynes ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Phenotypic Variation ◽  
Molecular Mimicry ◽  
Gastric Epithelial Cell ◽  
Epithelial Cell Surface

Intestinal Absorption of Macromolecules and Epithelial Cell Surface Glycosylation in Suckling Rats Nursed on Mothers Fed Low-Protein Diet-I

Neonatology ◽  
1990 ◽  
Vol 58 (2) ◽  
pp. 104-111 ◽  
Author(s):  
Harjit S. Babbar ◽  
Virender M.S. Jaswal ◽  
Reena Gupta ◽  
Akhtar Mahmood
Keyword(s):  
Epithelial Cell ◽  
Cell Surface ◽  
Intestinal Absorption ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Suckling Rats ◽  
Low Protein ◽  
Epithelial Cell Surface
Sign in / Sign up

Export Citation Format

Share Document