Species specific shoot regeneration response of cotyledonary explants of Brassicas

1988 ◽  
Vol 7 (2) ◽  
pp. 104-106 ◽  
Author(s):  
S. B. Narasimhulu ◽  
V. L. Chopra
Keyword(s):  
Shoot Regeneration ◽  
Cotyledonary Explants ◽  
Regeneration Response ◽  
Species Specific

Multiple Shoot Regeneration Response of Recalcitrant Cotton (Gossypism hirsutum L.) Cultivar CIM-443

2004 ◽  
Vol 7 (8) ◽  
pp. 1371-1375 ◽  
Author(s):  
Rozina M. Ali ◽  
Tayyab Husnain . ◽  
Syed Sarfraz Hussain . ◽  
Nasir Mahmood . ◽  
Sheikh Riazuddin .
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Regeneration Response ◽  
Multiple Shoot Regeneration

scholarly journals Studies on High Frequency Shoot Regeneration in Sesame (Sesamum indicum L.)

2012 ◽  
Vol 21 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Bangaramma S. Wadeyar ◽  
R. Lokesha
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Plant Tissue ◽  
High Frequency ◽  
Sesamum Indicum ◽  
Direct Seeding ◽  
The Other ◽  
Seeding Method ◽  
Regeneration Response ◽  
High Sucrose

High frequency shoot regeneration was attempted in sesame (Sesamum indicum L. Pedaliaceae), using five genotypes/varieties viz. Tumkur and Gulbarga Locals (land races), W-II, E-8 and DS-1 (varieties). The hypocotyl-derived callus obtained through direct seeding method was placed on MS with five different treatments viz. pre-culture of callus on high sucrose (6 - 9%) for two weeks and transferring on to plain MS with 3% sucrose, MS with variable concentrations of TDZ alone, MS with constant TDZ and variable concentrations of BA and IAA, MS with variable concentrations of BA and IAA without TDZ, MS with ABA and AgNO3 and MS with BAP, NAA and AgNO3.  Highest frequency of cent per cent shoot regeneration was initiated in variety DS-1on MS containing NAA 2.5 mg/l, BAP 3.5 mg/l and 20 µM AgNO3 with 2.50 mean shoots/callus followed by 91.6% in variety W-II on MS containing 25 µM TDZ with 2.20 mean shoots/callus. The other treatments had poor shoot regeneration response. The results have been discussed in the light of sesame improvement through biotechnological ways.   Key words: Sesamum indicum, hypocotyl, high frequency,  shoot regeneration   D. O. I. 10.3329/ptcb.v21i1.9562   Plant Tissue Cult. & Biotech. 21(1): 45-52, 2011 (June)

Factors influencing shoot regeneration from cotyledonary explants ofCucumis melo

1989 ◽  
Vol 18 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Randall P. Niedz ◽  
Sandra Schiller Smith ◽  
Kerry B. Dunbar ◽  
Christine T. Stephens ◽  
Harry H. Murakishi
Keyword(s):  
Shoot Regeneration ◽  
Factors Influencing ◽  
Cotyledonary Explants

Effect of genotype on shoot regeneration from cotyledonary explants of rapeseed (Brassica napus L.)

1994 ◽  
Vol 14 (1) ◽  
Author(s):  
Yuji Ono ◽  
Yoshihito Takahata ◽  
Norihiko Kaizuma
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration ◽  
Brassica Napus L ◽  
Cotyledonary Explants

scholarly journals Thidiazuron- and Zeatin-induced High-frequency Shoot Regeneration from Leaf and Shoot-tip Explants of Strawberry

2013 ◽  
Vol 23 (3) ◽  
pp. 276-281 ◽  
Author(s):  
Fatemeh Haddadi ◽  
Maheran Abd Aziz ◽  
Hossein Kamaladini ◽  
Seyed Ali Ravanfar
Keyword(s):  
Shoot Regeneration ◽  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Leaf Explant ◽  
Direct Organogenesis ◽  
Regeneration Response ◽  
Basal Salts ◽  
Shoot Tip Explants

Strawberry (Fragaria ×ananassa cv. Camarosa) was evaluated to determine a high-frequency shoot regeneration response for leaf and shoot-tip explants. For direct organogenesis from strawberry leaves, combinations of moderate concentrations of thidiazuron [TDZ (0, 2, and 4 μm)] and 6-benzylaminopurine [BAP (0, 4, and 9 μm)] added into medium containing Murashige and Skoog (MS) basal salts were compared. The most shoots regenerated per leaf explant were observed with 4-μm TDZ. Regeneration from shoot tips was evaluated with 0-, 2-, 4-, 8-, and 16-μm zeatin, kinetin, or 6-α,α-dimethylallylamino purine (2ip) tested individually. Optimum shoot proliferation was achieved from shoot-tip explants on medium containing 4-μm zeatin. Rooting was best without cytokinins in the medium; however, adequate rooting was obtained on the 4-μm zeatin treatment as well.

scholarly journals Genome-Wide Identification and Expression of the PIN Auxin Efflux Carrier Gene Family in Watermelon (Citrullus lanatus)

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 447
Author(s):  
Huaiguo Shang ◽  
Na Zhang ◽  
Zhouli Xie ◽  
Siyu Deng ◽  
Licong Yi ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Citrullus Lanatus ◽  
Expression Patterns ◽  
Genome Wide ◽  
Long Reads ◽  
Genome Wide Search ◽  
Fast Development ◽  
History Of ◽  
Pin Genes ◽  
Species Specific

As one of the world’s most popular fruits, watermelon (Citrus lanatus) is cultivated in more than 3 million hectares across the globe, with a yearly yield of more than 100 million tons. According to ‘97103’ genome version 1, a previous study has shown that the watermelon genome consists of 11 PIN genes. However, the higher quality ‘97103’ genome version 2 was recently assembled by using PacBio long reads with the benefit of fast development sequencing technology. Using this new assembly, we conducted a new genome-wide search for PIN genes in watermelon and compared it with cucumber and melon genomes. We identified nine, nine, and eight PINs in watermelon, cucumber, and melon, respectively. Phylogenetic analysis revealed a distinct evolutionary history of PIN proteins in watermelon, which is shown by the orphan PIN6 in watermelon. We further conducted an expression analysis of the watermelon PIN genes in silico and by qRT-PCR. ClaPIN6 might not play an essential role during shoot regeneration, which is closely related to species-specific evolution. However, the up-regulated expression patterns of ClaPIN1-1 and ClaPIN1-3 indicated their important roles during shoot regeneration. The result of this research will benefit future studies to understand the regulating mechanisms of auxin in watermelon shoot regeneration.

scholarly journals 084 Plant Preservative Mixture (PPM) can Reduce Shoot Regeneration from Leaf Explants of Selected Plants

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 455E-455 ◽  
Author(s):  
M.W. George ◽  
R.R. Tripepi
Keyword(s):  
Shoot Regeneration ◽  
Betula Pendula ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Regeneration ◽  
Dendranthema Grandiflora ◽  
The Media ◽  
Species Specific ◽  
Number Of Shoots

Plant preservative mixture (PPM) is a new broad-spectrum biocide that may be useful for plant tissue culture. The objective of this study was to determine if PPM interfered with adventitious shoot regeneration on leaf explants from several plant species. Leaf explants from Dendranthema grandiflora `Iridon', Betula pendula, Rhododendron catawbiense var. album and R.c. `America' were made from the top two apical leaves on the microshoots. In the first experiment, 0, 0.5, 1, 2, or 4 mL·L-1 PPM were added to species-appropriate regeneration media. In the second experiment, only mum leaf explants were placed on regeneration media containing 0, 0.1, 0.2, 0.3, or 0.4 mL·L-1 PPM. The percentage of explants forming shoots and the number of shoots per regenerating explant were recorded after 4, 6, and 10 weeks, for mum, birch, and rhododendron leaves, respectively. The percentages of shoot regeneration from birch and rhododendron leaf explants were unaffected by up to 4 mL·L-1 PPM, and the number of shoots formed per R.c. album explant were also unaffected by the tested concentrations of PPM. In contrast, the numbers of shoots formed on birch and `America' explants were reduced 48% and 25%, respectively, when 4 mL·L-1 PPM was used in the media. The percentages of shoot regeneration and number of shoots per explant were drastically reduced on mum explants when only 0.5 mL·L-1 PPM was used in the medium. In fact, 0.3 mL·L-1 PPM or higher reduced shoot formation by more than 5-fold. This study demonstrates that the effects of PPM on shoot regeneration from leaf explants are species specific.

scholarly journals Explants selection for in vitro propagation of Pachyrhizus erosus L.

2021 ◽  
Vol 13 (1) ◽  
pp. 10844
Author(s):  
Idowu A. OBISESAN ◽  
Ayobola M. A. SAKPERE ◽  
Bamidele J. AMUJOYEGBE ◽  
Michael S. AKINROPO
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Leaf Explants ◽  
Stem Explants ◽  
Ms Medium ◽  
Friable Callus ◽  
Pachyrhizus Erosus ◽  
Regeneration Response

Pachyrhizus erosus tuber is rich in protein asides its agronomical value as a legume, but the seeds by which it is propagated have very low viability. This study established sterilization protocol and effect of various concentrations of auxins and cytokinins on callus production and shoot regeneration from explants of P. erosus. Explants and seeds were sterilized using sodiumhypochlorite (NaClO) solution (5, 10 and 15% v/v) for 5 and 10 mins. Nodal, stem and leaf explants from in vitro germinated P. erosus and tuber from field grown plant were sterilized and cultured on Murashige and Skoog (MS) medium (control) and MS combined with different concentrations of auxins (NAA and 2, 4-D) and cytokinin (BA and Kinetin) and the cultured explants were monitored in terms of degree of callus formation, morphology and colour of callus and also for shoot induction. The results showed that seeds of P. erosus sterilized with 10% NaClO solution for 10 mins and germinated in vitro is the best way of getting sterile nodal, stem and leaf explants for the in vitro propagation of the plant, while tuber explants could be sterilized with 15% NaClO for 10 minutes. Nodal explants inoculated in MS medium supplemented with 1.0 mg/L BA gave the highest shoot regeneration response, while stem explants inoculated on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA also gave the highest amount of friable callus. The study concluded that in vitro germinated seeds were the best way of getting explant for P. erosus.

Effect of Medium pH on Shoot Regeneration from the Cotyledonary Explants of Tomato

2004 ◽  
Vol 4 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Poonam Bhatia . ◽  
Nanjappa Ashwath .
Keyword(s):  
Shoot Regeneration ◽  
Medium Ph ◽  
Cotyledonary Explants
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