Hormone-induced caulogenesis in long-term tobacco cell lines and its effect on nuclear DNA content

1981 ◽  
Vol 60 (1) ◽  
pp. 31-36 ◽  
Author(s):  
P. L. Traynor ◽  
S. M. Flashman
Keyword(s):  
Cell Lines ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Tobacco Cell

Stability of Nuclear DNA Content in Long-Term Callus Cultures of Loblolly Pine

1985 ◽  
pp. 319-319
Author(s):  
Chandrasekaran I. Franklin ◽  
Ralph L. Mott ◽  
Jerome P. Miksche
Keyword(s):  
Loblolly Pine ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Callus Cultures

scholarly journals Megakaryocytic cell line-specific hyperploidy by cytotoxic necrotizing factor bacterial toxins

Blood ◽  
1996 ◽  
Vol 88 (9) ◽  
pp. 3465-3473 ◽  
Author(s):  
KM Hudson ◽  
NC Denko ◽  
E Schwab ◽  
E Oswald ◽  
A Weiss ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Phorbol Ester ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Adherent Cell ◽  
Target Cells ◽  
Suspension Cells ◽  
Cytotoxic Necrotizing Factor

Cytotoxic necrotizing factor (CNF) toxins, isolated from certain Escherichia coli strains known to cause intestinal and extra intestinal infections, induce reorganization of the actin cytoskeleton and generate hyperploidy in adherent cell lines. We have examined the effect of CNF toxin on one of the few cell types that naturally increase nuclear DNA content, megakaryocytes. Our studies show that only hematopoietic cells capable of differentiating along the megakaryocyte lineage responded to the CNF2 toxin by becoming polyploid and by reorganizing actin. The K562, HEL, and CHRF-288–11 cell lines can be induced with phorbol ester to differentiate along the megakaryocyte lineage, and these cells also respond to the toxin with increased DNA content and actin cytoskeletal rearrangements. Interestingly, treatment of the K562 and HEL cell lines with CNF2 does not result in an increase in production of the megakaryocytic marker glycoprotein IIIa, unlike phorbol ester treatment. Conversely, two T-cell leukemic cell lines, CEM and Molt4, and the promyelocytic HL-60 cell line, which do not differentiate along the megakaryocyte lineage in response to phorbol myristate acetate, do not respond to CNF2, by increased expression of gpIIIa, increased nuclear DNA content, or actin reorganization. A potential target of these toxins, RhoA, is expressed by both megakaryocytic and nonmegakaryocytic cell lines, as shown by reverse transcription-polymerase chain reaction and Western blot. Although it is clear that the CNF toxins can affect a wide variety of adherent nonhematopoietic cell lines, we propose that the response to CNF, in terms of reorganizing actin structure and increase in DNA content in hematologic suspension cells, correlates with the capability of these target cells to differentiate along the megakaryocytic lineage.

scholarly journals MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT

2019 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Diego Pandeló José ◽  
José Marcello Salabert De Campos ◽  
Lyderson Facio Viccini ◽  
Emilly Ruas Alkimim ◽  
Marcelo De Oliveira Santos
Keyword(s):  
Flow Cytometry ◽  
Medicinal Plant ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Naphthalene Acetic Acid ◽  
Flow Cytometry Analysis ◽  
Ploidy Levels

Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry.

scholarly journals Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials

2000 ◽  
Vol 20 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Jean Dufer ◽  
Marie-France Poupon ◽  
Sonia Yatouji
Keyword(s):  
Tumor Cells ◽  
Cell Lines ◽  
Dna Content ◽  
Nuclear Dna ◽  
Chromatin Condensation ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Chromatin Pattern ◽  
Rhabdomyosarcoma Cell

There is a constant need of features able to characterize potentially metastatic cells among the heterogeneous cell subpopulations which constitute a tumor. Image cytometry of metastatic tumor cells give rise to variable results, partly because of a heterogeneous origin of cells, or potential drug effects. The aim of this work was to characterize nuclear changes observed in metastatic cell clones issuedin vitrofrom the same parental cell population The nuclear phenotypes of 6 cell sublines isolated from a rat rhabdomyosarcoma cell line and differing in their metastatic ability were evaluated by image cytometry on Feulgen‐stained preparations. Densitometric [5], geometric [3] and textural [9] features were computed from each nuclear image. For each cell subline, a metastatic score, ranging from 0 to 10, was calculated on the basis ofin vitroinvasivity data, by measuring the number of pulmonary metastases observed after s.c. graft of tumor cells in rats. Data obtained were compared to karyotype, growth characteristics, and oncogene expressions of cell lines. The nuclear DNA content, the chromosome numbers, the cell sublines doubling times, and the distribution of cells within the cell cycle appear unrelated with this score. On the contrary, increase in metastatic ability is accompanied by changes in chromatin pattern as assessed by textural features. Progressive increase in chromatin condensation can be observed in cell sublines with increasing metastatic score. These results were confirmed by an unsupervised multivariate partitioning of rhabdomyosarcoma cells which identified two separate subsets whose distributions within the analyzed cell lines correlate with their metastatic ability. These data suggest that, in rat rhabdomyosarcoma cell sublines, metastatic ability could be associated with nuclear morphological changes at the level of chromatin texture.

Nuclear DNA content and chromatin texture in multidrug-resistant human leukemic cell lines

1995 ◽  
Vol 60 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Jean Dufer ◽  
Christine Millot-Broguo ◽  
Zohra Oum Hamed ◽  
Francloise Lialtaud-Roger ◽  
Philippe Joly ◽  
...  
Keyword(s):  
Cell Lines ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Leukemic Cell ◽  
Multidrug Resistant ◽  
Human Leukemic Cell ◽  
Leukemic Cell Lines ◽  
Chromatin Texture

Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer

1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Dna Content ◽  
Prognostic Value ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Follicular Thyroid Cancer
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