Detection of oxygen activation and determination of the activity of antioxidants towards reactive oxygen species by use of the chemiluminigenic probes luminol and lucigenin

1987 ◽  
Vol 60 (1-3) ◽  
pp. 158-162 ◽  
Author(s):  
Regine Kahl ◽  
Andreas Weimann ◽  
Sabine Weinke ◽  
Alfred G. Hildebrandt
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Activation ◽  
Oxygen Species

UV-Vis Spectrophotometrical and Analytical Methodology for the Determination of Singlet Oxygen in New Antibacterials Drugs

2007 ◽  
Vol 2 ◽  
pp. 117739010700200 ◽  
Author(s):  
Tamara Zoltan ◽  
Franklin Vargas ◽  
Carla Izzo
Keyword(s):  
Reactive Oxygen Species ◽  
Singlet Oxygen ◽  
Nalidixic Acid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Inexpensive Method ◽  
Analytical Methodology ◽  
First Time

We have determined and quantified spectrophotometrically the capacity of producing reactive oxygen species (ROS) as 1O2 during the photolysis with UV-A light of 5 new synthesized naphthyl ester derivates of well-known quinolone antibacterials (nalidixic acid (1), cinoxacin (2), norfloxacin (3), ciprofloxacin (4) and enoxacin (5)). The ability of the naphthyl ester derivatives (6-10) to generate singlet oxygen were detecting and for the first time quantified by the histidine assay, a sensitive, fast and inexpensive method. The following tendency of generation of singlet oxygen was observed: compounds 7 >10 > 6 > 8 > 9 >> parent drugs 1-5.

Solid-phase supported profluorescent nitroxide probe for the determination of aerosol-borne reactive oxygen species

Talanta ◽  
2013 ◽  
Vol 116 ◽  
pp. 1033-1039 ◽  
Author(s):  
Mohamad Sleiman ◽  
Hugo Destaillats ◽  
Lara A. Gundel
Keyword(s):  
Reactive Oxygen Species ◽  
Solid Phase ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Nitroxide Probe

scholarly journals Fluorophotometric Determination of Hydrogen Peroxide and Other Reactive Oxygen Species with Fluorescein Hydrazide (FH) and Its Crystal Structure

2008 ◽  
Vol 56 (7) ◽  
pp. 977-981 ◽  
Author(s):  
Ryosuke Nakahara ◽  
Tsuyoshi Fujimoto ◽  
Mitsunobu Doi ◽  
Kanako Morita ◽  
Takako Yamaguchi ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Reactive Oxygen ◽  
Oxygen Species

A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species

Luminescence ◽  
2006 ◽  
Vol 21 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Daniela Komrskova ◽  
Antonin Lojek ◽  
Jan Hrbac ◽  
Milan Ciz
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Capacity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Chemical Systems

scholarly journals Level of reactive oxygen species induced by p21WAF(1)/CIP(1)is critical for the determination of cell fate

2009 ◽  
Vol 100 (7) ◽  
pp. 1275-1283 ◽  
Author(s):  
Takafumi Inoue ◽  
Kiyoko Kato ◽  
Hidenori Kato ◽  
Kazuo Asanoma ◽  
Ayumi Kuboyama ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Fate ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals A Monitoring of Allantoin, Uric Acid, and Malondialdehyde Levels in Plasma and Erythrocytes After Ten Minutes of Running Activity

2014 ◽  
pp. 753-762
Author(s):  
R. KANĎÁR ◽  
X. ŠTRAMOVÁ ◽  
P. DRÁBKOVÁ ◽  
J. KŘENKOVÁ
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Uric Acid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intense Exercise ◽  
Suitable Candidate ◽  
Running Activity ◽  
Plasma Malondialdehyde

Uric acid is the final product of human purine metabolism. It was pointed out that this compound acts as an antioxidant and is able to react with reactive oxygen species forming allantoin. Therefore, the measurement of allantoin levels may be used for the determination of oxidative stress in humans. The aim of the study was to clarify the antioxidant effect of uric acid during intense exercise. Whole blood samples were obtained from a group of healthy subjects. Allantoin, uric acid, and malondialdehyde levels in plasma and erythrocytes were measured using a HPLC with UV/Vis detection. Statistical significant differences in allantoin and uric acid levels during short-term intense exercise were found. Immediately after intense exercise, the plasma allantoin levels increased on the average of 200 % in comparison to baseline. Plasma uric acid levels increased slowly, at an average of 20 %. On the other hand, there were no significant changes in plasma malondialdehyde. The results suggest that uric acid, important antioxidant, is probably oxidized by reactive oxygen species to allantoin. Therefore allantoin may be suitable candidate for a marker of acute oxidative stress.

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