The mucous coat on gill lamellae of rainbow trout (Oncorhynchus mykiss)

1994 ◽  
Vol 275 (1) ◽  
pp. 187-193 ◽  
Author(s):  
J. S. Lumsden ◽  
H. W. Ferguson ◽  
V. E. Ostland ◽  
P. J. Byrne
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mucous Coat ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Gill Lamellae

scholarly journals A practical guide to unbiased quantitative morphological analyses of the gills of rainbow trout (Oncorhynchus mykiss) in ecotoxicological studies

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243462
Author(s):  
Sonja Fiedler ◽  
Hannah Wünnemann ◽  
Isabel Hofmann ◽  
Natalie Theobalt ◽  
Annette Feuchtinger ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Light Sheet ◽  
Objective Evaluation ◽  
Electron Microscopic ◽  
Experimental Animals ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Aquatic Pollutants ◽  
Broad Variety ◽  
Gill Lamellae

Rainbow trout (Oncorhynchus mykiss) are frequently used as experimental animals in ecotoxicological studies, in which they are experimentally exposed to defined concentrations of test substances, such as heavy metals, pesticides, or pharmaceuticals. Following exposure to a broad variety of aquatic pollutants, early morphologically detectable toxic effects often manifest in alterations of the gills. Suitable methods for an accurate and unbiased quantitative characterization of the type and the extent of morphological gill alterations are therefore essential prerequisites for recognition, objective evaluation and comparison of the severity of gill lesions. The aim of the present guidelines is to provide practicable, standardized and detailed protocols for the application of unbiased quantitative stereological analyses of relevant morphological parameters of the gills of rainbow trout. These gill parameters inter alia include the total volume of the primary and secondary gill lamellae, the surface area of the secondary gill lamellae epithelium (i.e., the respiratory surface) and the thickness of the diffusion barrier. The featured protocols are adapted to fish of frequently used body size classes (300–2000 g). They include well-established, conventional sampling methods, probes and test systems for unbiased quantitative stereological analyses of light- and electron microscopic 2-D gill sections, as well as the application of modern 3-D light sheet fluorescence microscopy (LSFM) of optically cleared gill samples as an innovative, fast and efficient quantitative morphological analysis approach. The methods shown here provide a basis for standardized and representative state-of-the-art quantitative morphological analyses of trout gills, ensuring the unbiasedness and reproducibility, as well as the intra- and inter-study comparability of analyses results. Their broad implementation will therefore significantly contribute to the reliable identification of no observed effect concentration (NOEC) limits in ecotoxicological studies and, moreover, to limit the number of experimental animals by reduction of unnecessary repetition of experiments.

scholarly journals Effects of cypermethrin on rainbow trout (Oncorhynchus mykiss) 

2012 ◽  
Vol 51 (No. 10) ◽  
pp. 469-476 ◽  
Author(s):  
J. Velisek ◽  
T. Wlasow ◽  
P. Gomulka ◽  
Z. Svobodova ◽  
R. Dobsikova ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Histopathological Examination ◽  
Toxicity Tests ◽  
Control Group ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Acute Toxicity Tests ◽  
Gill Lamellae ◽  
Experimental Group ◽  
Neutrophile Granulocytes

The aim of this study was to assess the effect of cypermethrin [(R,S)-&alpha;-cyano-3-phenoxybenzyl (1RS)-cis,tra-3-(2,2-dichlorovinyl)-2,2-dimethyl cyclopropane carboxylate] on rainbow trout (Oncorhynchus mykiss). The effect was assessed on the basis of the results of acute toxicity tests and on the comparison of results of haematological, biochemical and histopathological tissue examinations of a control and experimental group exposed to Alimetrine 10 EM pesticide preparation (active substance 100 g/l of cypermethrin). The acute semistatical toxicity test lasting 96 h was performed on rainbow trout juveniles. The 96hLC50 value of Alimethrine 10 EM was 31.4&nbsp;&micro;g/l. Examination of erythrocyte, leukocyte and biochemical profile and histopathological tissue examination was performed on 15 control and 15 experimental specimens of one-to-two-year-old rainbow trout after 96 h of exposure to Alimetrine 10 EM in the concentration of 31.4 &micro;g/l. The experimental group showed significantly higher values (P &lt; 0.01) of plasma ammonia (NH<sub>3</sub>), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), lactate (LACT) and significantly lower (P &lt; 0.01) values of alkaline phosphatase (ALP) compared to the control group. Also, a significant decrease in count of developmental forms of myeloid sequence, and segmented neutrophile granulocytes in the experimental group were found. Teleangioectasiae of secondary gill lamellae and degeneration of hepatocytes were observed with histopathological examination. No histopathological changes were demonstrated in tissues (skin, spleen, cranial and caudal kidney) of rainbow trout following exposure to cypermethrine. The cypermethrine-based Alimethrinee 10 EM pesticide preparation was classified among substances strongly toxic for fish.

Cryopreservation of rainbow trout(Oncorhynchus mykiss)Blastomeres

1993 ◽  
Vol 6 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Eric Nilsson ◽  
Joseph G. Cloud
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss

The essential oils of some medicinal plants on the immune system of rainbow trout (Oncorhynchus mykiss)

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Ghasemi Pirbalouti ◽  
E Pirali ◽  
G Pishkar ◽  
S Mohammadali Jalali ◽  
M Reyesi ◽  
...  
Keyword(s):  
Immune System ◽  
Rainbow Trout ◽  
Medicinal Plants ◽  
Oncorhynchus Mykiss ◽  
Essential Oils ◽  
Rainbow Trout Oncorhynchus Mykiss

Nuclear distribution of genotoxic damage in rainbow trout (Oncorhynchus mykiss) sperm after cryopreservation

2014 ◽  
Author(s):  
Silvia Gonzalez-Rojo ◽  
Cristina Fernandez-Diez ◽  
Marta Lombo ◽  
Vanesa Robles Rodriguez ◽  
Herraez Maria Paz
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Genotoxic Damage ◽  
Nuclear Distribution ◽  
Rainbow Trout Oncorhynchus Mykiss
Sign in / Sign up

Export Citation Format

Share Document