Prospect for enzyme therapy in glycogenosis II variants: a study on cultured muscle cells

1988 ◽  
Vol 235 (7) ◽  
pp. 392-396 ◽  
Author(s):  
A. T. Ploeg ◽  
P. A. Bolhuis ◽  
R. A. Wolterman ◽  
J. W. Visser ◽  
M. C. B. Loonen ◽  
...  
Keyword(s):  
Muscle Cells ◽  
Enzyme Therapy ◽  
Glycogenosis Ii

Ultrastructure of myoepithelial cell in parotid gland

1988 ◽  
Vol 46 ◽  
pp. 342-343
Author(s):  
C. N. Sun
Keyword(s):  
Parotid Gland ◽  
Osmium Tetroxide ◽  
Individual Cell ◽  
Branching Processes ◽  
Muscle Cells ◽  
Myoepithelial Cells ◽  
Uranyl Acetate ◽  
Thin Sections ◽  
Gland Carcinoma ◽  
Parotid Gland Carcinoma

Myoepithelial cells have been observed in the prostate, harderian, apocrine, exocrine sweat and mammary glands. Such cells and their numerous branching processes form basket-like structures around the glandular acini. Their shapes are quite different from structures seen either in spindleshaped smooth muscle cells or skeletal muscle cells. These myoepithelial cells lie on the epithelial side of the basement membrane in the glands. This presentation describes the ultrastructure of such myoepithelial cells which have been found also in the parotid gland carcinoma from a 45-year old patient.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4 percent glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate. Ultrastructurally, the pattern of each individual cell showed wide variations.

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

Pyrrolidine dithiocarbamate abrogates spontaneous NF-κB activation and IL-8 transcription in Crohn's disease intestinal smooth muscle cells

2001 ◽  
Vol 120 (5) ◽  
pp. A316-A316
Author(s):  
R NATARAJAN ◽  
B FISHER ◽  
S GHOSH ◽  
M GRAHAM ◽  
R DIEGELMANN ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Intestinal Smooth Muscle ◽  
Pyrrolidine Dithiocarbamate

Effect of acid-induced mucosal injury on isolated circular and longitudinal smooth muscle cells of the opossum esophagus

2001 ◽  
Vol 120 (5) ◽  
pp. A218-A219
Author(s):  
R WELLS ◽  
G MORRIS ◽  
W PATERSON
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Mucosal Injury ◽  
Longitudinal Smooth Muscle

173: Lipid Rafts are Negative Regulators of TGFβ Signaling in Ureteral Smooth Muscle Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 46-46
Author(s):  
Carlos R. Estrada ◽  
Theodora Danciu ◽  
Maximilian Stehr ◽  
Joseph Khoury ◽  
Keith R. Solomon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Lipid Rafts ◽  
Muscle Cells ◽  
Negative Regulators ◽  
Tgfβ Signaling

1450: Diabetes-Related Alterations in Connexin43-Derived GAP Junction Permeability in Short Term Cultures of RAT Corporal Vascular Smooth Muscle Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 382-382
Author(s):  
George Christ ◽  
Hong-Zhan Wang ◽  
Vergas Valiunas ◽  
Peter Brink ◽  
Stony Brook
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Gap Junction ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Short Term
Sign in / Sign up

Export Citation Format

Share Document