Patterns and projections of crustacean cardioactive-peptide-immunoreactice neurones of the terminal ganglion of crayfish

1993 ◽  
Vol 272 (3) ◽  
pp. 473-485 ◽  
Author(s):  
Uwe Audebm ◽  
Anke Trube ◽  
Heinrich Dircksen
Keyword(s):  
Terminal Ganglion ◽  
Crustacean Cardioactive Peptide

Cloning of crustacean cardioactive peptide and its functional verification under low-salt adaptation in swimming crab (Portunus trituberculatus)

2019 ◽  
Vol 26 (2) ◽  
pp. 261
Author(s):  
Dongfang SUN ◽  
Jianjian LYU ◽  
Baoquan GAO ◽  
Pengpeng HUAN ◽  
Ying CAI ◽  
...  
Keyword(s):  
Portunus Trituberculatus ◽  
Functional Verification ◽  
Salt Adaptation ◽  
Swimming Crab ◽  
Low Salt ◽  
Crustacean Cardioactive Peptide

IDENTIFICATION, SEQUENCE AND EXPRESSION OF A CRUSTACEAN CARDIOACTIVE PEPTIDE (CCAP) GENE IN THE MOTHMANDUCA SEXTA

2001 ◽  
Vol 204 (16) ◽  
pp. 2803-2816 ◽  
Author(s):  
P. K. LOI ◽  
S. A. EMMAL ◽  
Y. PARK ◽  
N. J. TUBLITZ
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Gene Structure ◽  
Expression Patterns ◽  
Amino Acid Residues ◽  
Coding Region ◽  
Genome Database ◽  
Reading Frame ◽  
Crustacean Cardioactive Peptide ◽  
Embryonic Life

SUMMARYThe crustacean cardioactive peptide (CCAP) gene was isolated from the tobacco hawkmoth Manduca sexta. The gene has an open reading frame of 125 amino acid residues containing a single, complete copy of CCAP. Analysis of the gene structure revealed three introns interrupting the coding region. A comparison of the M. sexta CCAP gene with the Drosophila melanogaster genome database reveals significant similarities in sequence and gene structure.The spatial and temporal expression patterns of the CCAP gene in the M. sexta central nervous system were determined in all major post-embryonic stages using in situ hybridization techniques. The CCAP gene is expressed in a total of 116 neurons in the post-embryonic M. sextacentral nervous system. Nine pairs of cells are observed in the brain, 4.5 pairs in the subesophageal ganglion, three pairs in each thoracic ganglion(T1-T3), three pairs in the first abdominal ganglion (A1), five pairs each in the second to sixth abdominal ganglia (A2-A6) and 7.5 pairs in the terminal ganglion. The CCAP gene is expressed in every ganglion in each post-embryonic stage, except in the thoracic ganglia of first- and second-instar larvae. The number of cells expressing the CCAP gene varies during post-embryonic life,starting at 52 cells in the first instar and reaching a maximum of 116 shortly after pupation. One set of thoracic neurons expressing CCAP mRNA shows unusual variability in expression levels immediately prior to larval ecdysis. Using previously published CCAP immunocytochemical data, it was determined that 91 of 95 CCAP-immunopositive neurons in the M. sexta central nervous system also express the M. sexta CCAP gene, indicating that there is likely to be only a single CCAP gene in M. sexta.

scholarly journals Prothoracicostatic Activity of the Ecdysis-Regulating Neuropeptide Crustacean Cardioactive Peptide (CCAP) in the Desert Locust

2021 ◽  
Vol 22 (24) ◽  
pp. 13465
Author(s):  
Lina Verbakel ◽  
Cynthia Lenaerts ◽  
Rania Abou El Asrar ◽  
Caroline Zandecki ◽  
Evert Bruyninckx ◽  
...  
Keyword(s):  
Schistocerca Gregaria ◽  
Expression Profiles ◽  
Functional Integration ◽  
Motor Program ◽  
Prothoracic Gland ◽  
Desert Locust ◽  
Endocrine Organs ◽  
Crustacean Cardioactive Peptide ◽  
Genes Encoding ◽  
Ecdysteroid Biosynthesis

Accurate control of innate behaviors associated with developmental transitions requires functional integration of hormonal and neural signals. Insect molting is regulated by a set of neuropeptides, which trigger periodic pulses in ecdysteroid hormone titers and coordinate shedding of the old cuticle during ecdysis. In the current study, we demonstrate that crustacean cardioactive peptide (CCAP), a structurally conserved neuropeptide described to induce the ecdysis motor program, also exhibits a previously unknown prothoracicostatic activity to regulate ecdysteroid production in the desert locust, Schistocerca gregaria. We identified the locust genes encoding the CCAP precursor and three G protein-coupled receptors that are activated by CCAP with EC50 values in the (sub)nanomolar range. Spatiotemporal expression profiles of the receptors revealed expression in the prothoracic glands, the endocrine organs where ecdysteroidogenesis occurs. RNAi-mediated knockdown of CCAP precursor or receptors resulted in significantly elevated transcript levels of several Halloween genes, which encode ecdysteroid biosynthesis enzymes, and in elevated ecdysteroid levels one day prior to ecdysis. Moreover, prothoracic gland explants exhibited decreased secretion of ecdysteroids in the presence of CCAP. Our results unequivocally identify CCAP as the first prothoracicostatic peptide discovered in a hemimetabolan species and reveal the existence of an intricate interplay between CCAP signaling and ecdysteroidogenesis.

scholarly journals Characterization of Crustacean Cardioactive Peptide as a Novel Insect Midgut Factor: Isolation, Localization, and Stimulation of α-Amylase Activity and Gut Contraction

Endocrinology ◽  
2004 ◽  
Vol 145 (12) ◽  
pp. 5671-5678 ◽  
Author(s):  
Tsubasa Sakai ◽  
Honoo Satake ◽  
Hiroyuki Minakata ◽  
Makio Takeda
Keyword(s):  
Amylase Activity ◽  
Insect Midgut ◽  
Crustacean Cardioactive Peptide ◽  
Stimulation Of

Molecular Cloning, Characterization, and Expression of Crustacean Cardioactive Peptide in Oriental River Prawn, Macrobrachium nipponense , during Acute Hypoxia and Reoxygenation

2018 ◽  
Vol 49 (2) ◽  
pp. 356-365 ◽  
Author(s):  
Dongyan Shan ◽  
Hongtuo Fu ◽  
Hui Qiao ◽  
Shengming Sun ◽  
Wenyi Zhang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Acute Hypoxia ◽  
Macrobrachium Nipponense ◽  
Crustacean Cardioactive Peptide ◽  
Oriental River Prawn

scholarly journals Modulation of Force during Locomotion: Differential Action of Crustacean Cardioactive Peptide on Power-Stroke and Return- Stroke Motor Neurons

1997 ◽  
Vol 17 (18) ◽  
pp. 6872-6883 ◽  
Author(s):  
Brian Mulloney ◽  
Hisaaki Namba ◽  
Hans-Jürgen Agricola ◽  
Wendy M. Hall
Keyword(s):  
Motor Neurons ◽  
Power Stroke ◽  
Return Stroke ◽  
Crustacean Cardioactive Peptide ◽  
Differential Action

scholarly journals Isolation and Characterization of an Adipokinetic Hormone Release-Inducing Factor in Locusts: The Crustacean Cardioactive Peptide

Endocrinology ◽  
1997 ◽  
Vol 138 (1) ◽  
pp. 138-142 ◽  
Author(s):  
D. Veelaert ◽  
P. Passier ◽  
B. Devreese ◽  
J. Vanden Broeck ◽  
J. Van Beeumen ◽  
...  
Keyword(s):  
Hormone Release ◽  
Adipokinetic Hormone ◽  
Isolation And Characterization ◽  
Crustacean Cardioactive Peptide
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