Movement within and between different habitats by the portunid crab Scylla serrata

Crab meat is widely consumed in several countries around the world. However, when consumed, crab meats are frequent cause of allergic reactions throughout the world. Scylla serrata is among the most common mud crab in Malaysia. In a previous study two major allergens of mud crab at 36 and 41 kDa was identified. Thus, the aim of this study is to further identify these major allergens by a proteomic approach. Protein extract was prepared and resolved by 2-dimensional electrophoresis (2-DE). Immunoblotting was then performed using reactive sera from patients with crab allergy. Major allergenic spots were then excised from the 2-DE gel and analysed by mass spectrometry. The 2-DE profile of the extract revealed approximately >100 protein spots between pH of 4.00 to 8.00. Mass spectrometry analysis has identified the 36 and 41 kDa proteins as tropomyosin and arginine kinase, respectively. Our findings indicated that tropomyosin and arginine kinase play a major role in allergic reaction to mud crab meat among local patients with crab meat allergy, and should be included in diagnostics and therapeutic strategies of this allergy.

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ẢNH HƯỞNG CỦA NHIỆT ĐỘ ĐẾN TỶ LỆ SỐNG VÀ THỜI GIAN BIẾN THÁI ẤU TRÙNG CUA XANH Scylla serrata (Forskal, 1775) GIAI ĐOẠN ZOEA ĐẾN MEGALOPA.

HUE UNIVERSITY JOURNAL OF SCIENCE AGRICULTURE AND RURAL DEVELOPMENT ◽

10.26459/jard.v104i5.2954 ◽

2015 ◽

Vol 104 (5) ◽

Author(s):

Nguyễn Đức Thành ◽

Nguyễn Duy Quỳnh Trâm ◽

Nguyễn Khoa Huy Sơn ◽

Phạm Thị Phương Lan

Keyword(s):

Scylla Serrata

Nghiên cứu này nhằm đánh giá ảnh hưởng các mức nhiệt độ lên tỷ lệ sống và thời gian biến thái của ấu trùng cua xanh Scylla serata giai đoạn Zoea đến Megalopa. Thí nghiệm được tiến hành từ giai đoạn Zoea2, theo kiểu khối ngẫu nhiên hoàn toàn với 4 nghiệm thức tương ứng với 4 mức nhiệt độ 25, 27, 29 và 31 0C. Kết quả thí nghiệm cho thấy có sự ảnh hưởng khác biệt về tỷ lệ sống và thời gian biến thái giữa các mức nhiệt độ với mức tin cậy p<0,05 . Khoảng nhiệt độ từ 270C đến 290C cho tỷ lệ sống ấu trùng cao, nghiệm thức nhiệt độ 290C cho tỷ lệ sống của ấu trùng cao nhất ở giai đoạn Zoea2 và Zoea4. Nhiệt độ thí nghiệm càng tăng thời gian biến thái của ấu trùng càng ngắn. Từ khóa: cua xanh, ấu trùng, nhiệt độ, tỷ lệ sống, thời gian biến thái

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The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

Aquatic Science and Technology ◽

10.5296/ast.v5i2.11594 ◽

2017 ◽

Vol 5 (2) ◽

pp. 1

Author(s):

Mulyati Mulyati ◽

Suryati Suryati ◽

Irfani Baga

Keyword(s):

Survival Rate ◽

Sea Water ◽

Challenge Test ◽

Probiotic Bacteria ◽

Pathogenicity Test ◽

Inhibitory Ability ◽

Significant Difference ◽

Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

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Co-Infection of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in the Wild Crustaceans of Andaman and Nicobar Archipelago, India

Viruses ◽

10.3390/v13071378 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1378

Author(s):

Kandasamy Saravanan ◽

Jayasimhan Praveenraj ◽

Rajendran Kiruba-Sankar ◽

Varsha Devi ◽

Utpal Biswas ◽

...

Keyword(s):

Inclusion Bodies ◽

White Spot Syndrome Virus ◽

Penaeus Monodon ◽

White Spot ◽

Scylla Serrata ◽

Histopathological Analysis ◽

Necrosis Virus ◽

High Sequence Identity ◽

In The Wild ◽

White Spot Syndrome

The present study was intended to screen the wild crustaceans for co-infection with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in Andaman and Nicobar Archipelago, India. We screened a total of 607 shrimp and 110 crab samples using a specific polymerase chain reaction, and out of them, 82 shrimps (13.5%) and 5 (4.5%) crabs were found positive for co-infection of IHHNV and WSSV. A higher rate of co-infection was observed in Penaeus monodon and Scylla serrata than other shrimp and crab species. The nucleotide sequences of IHHNV and WSSV obtained from crab in this present study exhibited very high sequence identity with their counterparts retrieved from various countries. Histopathological analysis of the infected shrimp gill sections further confirmed the eosinophilic intra-nuclear cowdry type A inclusion bodies and basophilic intra-nuclear inclusion bodies characteristics of IHHNV and WSSV infections, respectively. The present study serves as the first report on co-infection of WSSV and IHHNV in Andaman and Nicobar Archipelago, India and accentuates the critical need for continuous monitoring of wild crustaceans and appropriate biosecurity measures for brackishwater aquaculture.

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