Bisulfite interacts with binding sites of the auxin-transport inhibitor N-1-naphthylphthalamic acid

Planta ◽  
1988 ◽  
Vol 176 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Michael Thein ◽  
Wolfgang Michalke
Keyword(s):  
Binding Sites ◽  
Auxin Transport ◽  
Transport Inhibitor ◽  
Auxin Transport Inhibitor ◽  
Naphthylphthalamic Acid

An Auxin Transport Inhibitor Interferes With Unicellular Gravitropism in Protonemata of the Moss Ceratodon purpureus

Plant Biology ◽  
2001 ◽  
Vol 3 (4) ◽  
pp. 357-363 ◽  
Author(s):  
J. Schwuchow ◽  
W. Michalke ◽  
R. Hertel
Keyword(s):  
Auxin Transport ◽  
Ceratodon Purpureus ◽  
Transport Inhibitor ◽  
Auxin Transport Inhibitor

Corrigendum to: Auxin is required for pollination-induced ovary growth in Dendrobium orchids

2006 ◽  
Vol 33 (10) ◽  
pp. 981 ◽  
Author(s):  
Saichol Ketsa ◽  
Apinya Wisutiamonkul ◽  
Wouter G. van Doorn
Keyword(s):  
Auxin Transport ◽  
Isobutyric Acid ◽  
Signalling Pathway ◽  
Aminooxyacetic Acid ◽  
Ethylene Synthesis ◽  
Transport Inhibitor ◽  
Auxin Signalling ◽  
Auxin Transport Inhibitor ◽  
Ethylene Action ◽  
Ovary Growth

In Dendrobium and other orchids the ovule becomes mature long after pollination, whereas the ovary starts growing within two days of pollination. The signalling pathway that induces rapid ovary growth after pollination has remained elusive. We placed the auxin antagonist �-(p-chlorophenoxy) isobutyric acid (PCIB) or the auxin transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) on the stigma, before pollination. Both treatments nullified pollination-induced ovary growth. The ovaries also did not grow after similar stigma treatment with 1-methylcyclopropene (1-MCP), AgNO3 (both inhibitors of ethylene action), aminooxyacetic acid (AOA) or CoCl2 (which both inhibit ethylene synthesis), before pollination. Pollination could be replaced by placement of the auxin naphthylacetic acid (NAA) on the stigma. All mentioned inhibitors nullified the effect of NAA, indicating that if auxin is the initiator of ovary growth, it acts through ethylene. The results suggest that the pollination effect on ovary growth requires auxin (at least auxin transport and maybe also auxin signalling), and both ethylene synthesis and ethylene action.

scholarly journals Activation of Big Grain1 significantly improves grain size by regulating auxin transport in rice

2015 ◽  
Vol 112 (35) ◽  
pp. 11102-11107 ◽  
Author(s):  
Linchuan Liu ◽  
Hongning Tong ◽  
Yunhua Xiao ◽  
Ronghui Che ◽  
Fan Xu ◽  
...  
Keyword(s):  
Grain Size ◽  
Auxin Transport ◽  
Vascular Tissue ◽  
Plant Biomass ◽  
Key Factors ◽  
Auxin Distribution ◽  
New Strategy ◽  
Dna Insertion ◽  
Auxin Transport Inhibitor ◽  
Naphthylphthalamic Acid

Grain size is one of the key factors determining grain yield. However, it remains largely unknown how grain size is regulated by developmental signals. Here, we report the identification and characterization of a dominant mutant big grain1 (Bg1-D) that shows an extra-large grain phenotype from our rice T-DNA insertion population. Overexpression of BG1 leads to significantly increased grain size, and the severe lines exhibit obviously perturbed gravitropism. In addition, the mutant has increased sensitivities to both auxin and N-1-naphthylphthalamic acid, an auxin transport inhibitor, whereas knockdown of BG1 results in decreased sensitivities and smaller grains. Moreover, BG1 is specifically induced by auxin treatment, preferentially expresses in the vascular tissue of culms and young panicles, and encodes a novel membrane-localized protein, strongly suggesting its role in regulating auxin transport. Consistent with this finding, the mutant has increased auxin basipetal transport and altered auxin distribution, whereas the knockdown plants have decreased auxin transport. Manipulation of BG1 in both rice and Arabidopsis can enhance plant biomass, seed weight, and yield. Taking these data together, we identify a novel positive regulator of auxin response and transport in a crop plant and demonstrate its role in regulating grain size, thus illuminating a new strategy to improve plant productivity.

The white-tip nematode, Aphelenchoides besseyi, exhibits an auxin-orientated behaviour affecting its migration and propagation

Nematology ◽  
2014 ◽  
Vol 16 (7) ◽  
pp. 837-845 ◽  
Author(s):  
Hui Feng ◽  
Ying Shao ◽  
Li-hui Wei ◽  
Cun-yi Gao ◽  
Yi-jun Zhou
Keyword(s):  
Auxin Transport ◽  
Naphthaleneacetic Acid ◽  
Male Sterile ◽  
Auxin Level ◽  
Rice Plants ◽  
Transport Inhibitor ◽  
Large Numbers ◽  
Auxin Concentration ◽  
Aphelenchoides Besseyi ◽  
Auxin Transport Inhibitor

Aphelenchoides besseyi is an obligate parasite that often causes white-tip symptoms in rice plants. The nematode exhibits ectoparasitic behaviour with its infection rate matching the development of rice plants. Few studies have analysed how A. besseyi migration is influenced by chemical and host factors. Here, we focused on the effects of auxins on nematode migration and propagation. Exposure of A. besseyi to an auxin gradient created by a Pluronic F-127 gel resulted in nematode aggregation at the highest auxin concentration tested, 100 μm. Inoculation on the susceptible cv. Ningjing1 produced more nematodes than on the resistant rice cv. Tetep, which correlated with their endogenous auxin levels. Young panicles treated with 1-naphthaleneacetic acid produced more grains and nematodes, whereas plants treated with the auxin transport inhibitor, 2,3,5-triiodobenzoic acid, led to fewer nematodes in the seeds. In addition, A. besseyi rarely migrated and multiplied in the plants of the male sterile rice cv. Zhenshan97A, which had insufficient auxin level in pollen and thus could not generate any grains in most panicles. However, large numbers of nematodes were observed in seeds of cv. Zhenshan97A that had received pollens from the maintainer cv. Zhenshan97B. The results indicate that auxin might play a key role in the migration and propagation of A. besseyi.

Biokinetics and Efficacy of Aminocyclopyrachlor-Methyl Ester as Influenced by Diflufenzopyr

Weed Science ◽  
2014 ◽  
Vol 62 (3) ◽  
pp. 538-547 ◽  
Author(s):  
Jose J. Vargas ◽  
James T. Brosnan ◽  
Thomas C. Mueller ◽  
Dean A. Kopsell ◽  
William E. Klingeman ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Auxin Transport ◽  
Free Acid ◽  
Acid Metabolite ◽  
Black Nightshade ◽  
Species Accumulation ◽  
Transport Inhibitor ◽  
Auxin Transport Inhibitor ◽  
Time Point ◽  
Large Crabgrass

Research studies evaluated effects of the auxin transport inhibitor, diflufenzopyr, on the biokinetics and efficacy of aminocyclopyrachlor-methyl ester (AMCP-ME) applications to black nightshade and large crabgrass. Absorption, translocation, and metabolism of 14C-AMCP-ME was quantified with and without diflufenzopyr (35 g ai ha−1). Diflufenzopyr had minimal effects on translocation of radioactivity in either species. Accumulation of radioactivity in aboveground plant sections of black nightshade was greater than or equal to that in large crabgrass by 72 h after treatment (HAT). In both species, metabolism of 14C-AMCP-ME was rapid, as 60 to 78% of the extracted radioactivity was the free acid metabolite 8 HAT. In the greenhouse, black nightshade and large crabgrass were treated with AMCP-ME (9, 18, and 35 g ai ha−1) alone and in combination with diflufenzopyr (35 g ha−1). Mixtures of AMCP-ME plus diflufenzopyr did not increase large crabgrass control compared with AMCP-ME alone at any time. Diflufenzopyr (35 g ha−1) increased black nightshade control with AMCP-ME (18 and 35 g ha−1) 7 d after treatment (DAT). However, this increase in control was not observed 14 or 28 DAT. All treatments containing AMCP-ME controlled large crabgrass 70 to 79% 28 DAT compared with > 93% for black nightshade at the same time point.

scholarly journals Fine control of aerenchyma and lateral root development through AUX/IAA- and ARF-dependent auxin signaling

2019 ◽  
Vol 116 (41) ◽  
pp. 20770-20775 ◽  
Author(s):  
Takaki Yamauchi ◽  
Akihiro Tanaka ◽  
Hiroki Inahashi ◽  
Naoko K. Nishizawa ◽  
Nobuhiro Tsutsumi ◽  
...  
Keyword(s):  
Root Development ◽  
Molecular Mechanisms ◽  
Auxin Transport ◽  
Lateral Roots ◽  
Cortical Cell ◽  
Dominant Negative ◽  
Dominant Negative Effect ◽  
Auxin Signaling ◽  
Transport Inhibitor ◽  
Auxin Transport Inhibitor

Lateral roots (LRs) are derived from a parental root and contribute to water and nutrient uptake from the soil. Auxin/indole-3-acetic acid protein (AUX/IAA; IAA) and auxin response factor (ARF)-mediated signaling are essential for LR formation. Lysigenous aerenchyma, a gas space created by cortical cell death, aids internal oxygen transport within plants. Rice (Oryza sativa) forms lysigenous aerenchyma constitutively under aerobic conditions and increases its formation under oxygen-deficient conditions; however, the molecular mechanisms regulating constitutive aerenchyma (CA) formation remain unclear. LR number is reduced by the dominant-negative effect of a mutated AUX/IAA protein in the iaa13 mutant. We found that CA formation is also reduced in iaa13. We have identified ARF19 as an interactor of IAA13 and identified a lateral organ boundary domain (LBD)-containing protein (LBD1-8) as a target of ARF19. IAA13, ARF19, and LBD1-8 were highly expressed in the cortex and LR primordia, suggesting that these genes function in the initiation of CA and LR formation. Restoration of LBD1-8 expression recovered aerenchyma formation and partly recovered LR formation in the iaa13 background, in which LBD1-8 expression was reduced. An auxin transport inhibitor suppressed CA and LR formation, and a natural auxin stimulated CA formation in the presence of the auxin transport inhibitor. Our findings suggest that CA and LR formation are both regulated through AUX/IAA- and ARF-dependent auxin signaling. The initiation of CA formation lagged that of LR formation, which indicates that the formation of CA and LR are regulated differently by auxin signaling during root development in rice.

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