Sensitizing capacity of three methyl alkanesulphonates: a murine in vivo and in vitro model of allergic contact dermatitis

1990 ◽  
Vol 282 (7) ◽  
pp. 455-458 ◽  
Author(s):  
R. Fraginals ◽  
J. -P. Lepoittevin ◽  
C. Benezra
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
In Vitro Model ◽  
Vitro Model ◽  
Allergic Contact

A murine in vitro model of allergic contact dermatitis to sesquiterpene ?-methylene-?-butyrolactones

1992 ◽  
Vol 284 (5) ◽  
pp. 297-302 ◽  
Author(s):  
N. Alonso Blasi ◽  
R. Fraginals ◽  
J. -P. Lepoittevin ◽  
C. Benezra
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
In Vitro Model ◽  
Vitro Model ◽  
Allergic Contact

Airborne allergic contact dermatitis caused by Machaerium scleroxylon : Confirmation by in vivo and in vitro tests

2019 ◽  
Vol 81 (4) ◽  
pp. 296-298 ◽  
Author(s):  
Katharina Hansel ◽  
Nicola Murgia ◽  
Anna Russano ◽  
Francesco Crescenzi ◽  
Marta Tramontana ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
In Vitro Tests ◽  
Allergic Contact

In Vitro and In Vivo Testing Techniques for Allergic Contact Dermatitis

Dermatitis ◽  
1998 ◽  
Vol 9 (2) ◽  
pp. 111-118
Author(s):  
G. Frank Gerberick ◽  
Elizabeth E. Sikorski
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
In Vivo Testing ◽  
Testing Techniques ◽  
Allergic Contact

Xanthone suppresses allergic contact dermatitis in vitro and in vivo

2020 ◽  
Vol 78 ◽  
pp. 106061 ◽  
Author(s):  
Aye Aye ◽  
Young-Jae Song ◽  
Yong-Deok Jeon ◽  
Jong-Sik Jin
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
Allergic Contact

Red Grape Polyphenol Oral Administration improves Immune Response in Women Affected by Nickel-Mediated Allergic Contact Dermatitis

2020 ◽  
Vol 20 ◽  
Author(s):  
Thea Magrone ◽  
Emilio Jirillo ◽  
Manrico Magrone ◽  
Matteo Antonio Russo ◽  
Paolo Romita ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Oral Administration ◽  
Allergic Contact Dermatitis ◽  
Laboratory Data ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Red Grape ◽  
Allergic Contact ◽  
Drop Outs

Background: Our previous findings demonstrated that in vitro supplementation of polyphenols, extracted from seeds of red grape (Nero di Troia cultivar), to peripheral lymphomonocytes from patients affected by allergic contact dermatitis (ACD) to nickel (Ni) could reduce release of pro-inflammatory cytokines and nitric oxide (NO), while increasing levels of interleukin (IL)-10, an anti-inflammatory cytokine. Objective: To assess whether an intervention with oral administration of polyphenols leads to a reduction of peripheral biomarkers in ACD patients. Method: At T0, 25 patients affected by ACD to Ni were orally administered with 300 mg polyphenols prodie extracted from seeds of red grape (Nero di Troia cultivar) (NATUR-OX®) for 3 months (T1). Other 25 patients affected by ACD to Ni received placebo only for the same period of time. Serum biomarkers were analyzed at T0 and T1. In both groups seven drop outs were recorded. Result: At T1 in comparison to T0, in treated patients, values of IFN-γ, IL-4, IL-17, PTX3 and NO decreased, while IL-10 levels increased when compared with T0 values. Conversely, in placebo-treated patients no modifications of biomarkers were evaluated at T1. Conclusion: Present laboratory data rely on the anti-oxidant, anti-inflammatory and anti-allergic properties of polyphenols.

scholarly journals Generation of a Novel In Vitro Model to Study Endothelial Dysfunction from Atherothrombotic Specimens

2021 ◽  
Author(s):  
Susan Gallogly ◽  
Takeshi Fujisawa ◽  
John D. Hung ◽  
Mairi Brittan ◽  
Elizabeth M. Skinner ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Dysfunction ◽  
In Vitro Model ◽  
Umbilical Vein ◽  
Coronary Syndrome ◽  
Coronary Endothelial Cells ◽  
Vitro Model ◽  
Umbilical Vein Endothelial Cells

Abstract Purpose Endothelial dysfunction is central to the pathogenesis of acute coronary syndrome. The study of diseased endothelium is very challenging due to inherent difficulties in isolating endothelial cells from the coronary vascular bed. We sought to isolate and characterise coronary endothelial cells from patients undergoing thrombectomy for myocardial infarction to develop a patient-specific in vitro model of endothelial dysfunction. Methods In a prospective cohort study, 49 patients underwent percutaneous coronary intervention with thrombus aspiration. Specimens were cultured, and coronary endothelial outgrowth (CEO) cells were isolated. CEO cells, endothelial cells isolated from peripheral blood, explanted coronary arteries, and umbilical veins were phenotyped and assessed functionally in vitro and in vivo. Results CEO cells were obtained from 27/37 (73%) atherothrombotic specimens and gave rise to cells with cobblestone morphology expressing CD146 (94 ± 6%), CD31 (87 ± 14%), and von Willebrand factor (100 ± 1%). Proliferation of CEO cells was impaired compared to both coronary artery and umbilical vein endothelial cells (population doubling time, 2.5 ± 1.0 versus 1.6 ± 0.3 and 1.2 ± 0.3 days, respectively). Cell migration was also reduced compared to umbilical vein endothelial cells (29 ± 20% versus 85±19%). Importantly, unlike control endothelial cells, dysfunctional CEO cells did not incorporate into new vessels or promote angiogenesis in vivo. Conclusions CEO cells can be reliably isolated and cultured from thrombectomy specimens in patients with acute coronary syndrome. Compared to controls, patient-derived coronary endothelial cells had impaired capacity to proliferate, migrate, and contribute to angiogenesis. CEO cells could be used to identify novel therapeutic targets to enhance endothelial function and prevent acute coronary syndromes.

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