Airborne allergic contact dermatitis caused by Machaerium scleroxylon : Confirmation by in vivo and in vitro tests

2019 ◽  
Vol 81 (4) ◽  
pp. 296-298 ◽  
Author(s):  
Katharina Hansel ◽  
Nicola Murgia ◽  
Anna Russano ◽  
Francesco Crescenzi ◽  
Marta Tramontana ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
In Vitro Tests ◽  
Allergic Contact

In Vitro and In Vivo Testing Techniques for Allergic Contact Dermatitis

Dermatitis ◽  
1998 ◽  
Vol 9 (2) ◽  
pp. 111-118
Author(s):  
G. Frank Gerberick ◽  
Elizabeth E. Sikorski
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
In Vivo Testing ◽  
Testing Techniques ◽  
Allergic Contact

Xanthone suppresses allergic contact dermatitis in vitro and in vivo

2020 ◽  
Vol 78 ◽  
pp. 106061 ◽  
Author(s):  
Aye Aye ◽  
Young-Jae Song ◽  
Yong-Deok Jeon ◽  
Jong-Sik Jin
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
Allergic Contact

Red Grape Polyphenol Oral Administration improves Immune Response in Women Affected by Nickel-Mediated Allergic Contact Dermatitis

2020 ◽  
Vol 20 ◽  
Author(s):  
Thea Magrone ◽  
Emilio Jirillo ◽  
Manrico Magrone ◽  
Matteo Antonio Russo ◽  
Paolo Romita ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Oral Administration ◽  
Allergic Contact Dermatitis ◽  
Laboratory Data ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Red Grape ◽  
Allergic Contact ◽  
Drop Outs

Background: Our previous findings demonstrated that in vitro supplementation of polyphenols, extracted from seeds of red grape (Nero di Troia cultivar), to peripheral lymphomonocytes from patients affected by allergic contact dermatitis (ACD) to nickel (Ni) could reduce release of pro-inflammatory cytokines and nitric oxide (NO), while increasing levels of interleukin (IL)-10, an anti-inflammatory cytokine. Objective: To assess whether an intervention with oral administration of polyphenols leads to a reduction of peripheral biomarkers in ACD patients. Method: At T0, 25 patients affected by ACD to Ni were orally administered with 300 mg polyphenols prodie extracted from seeds of red grape (Nero di Troia cultivar) (NATUR-OX®) for 3 months (T1). Other 25 patients affected by ACD to Ni received placebo only for the same period of time. Serum biomarkers were analyzed at T0 and T1. In both groups seven drop outs were recorded. Result: At T1 in comparison to T0, in treated patients, values of IFN-γ, IL-4, IL-17, PTX3 and NO decreased, while IL-10 levels increased when compared with T0 values. Conversely, in placebo-treated patients no modifications of biomarkers were evaluated at T1. Conclusion: Present laboratory data rely on the anti-oxidant, anti-inflammatory and anti-allergic properties of polyphenols.

Imidazolidinyl urea activates mast cells via MRGPRX2 to induce non-histaminergic allergy

2021 ◽  
Author(s):  
Jiapan Gao ◽  
Delu Che ◽  
Xueshan Du ◽  
Yi Zheng ◽  
Huiling Jing ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Mast Cells ◽  
Pharmaceutical Products ◽  
Drug Induced ◽  
Inflammatory Infiltration ◽  
Local Inflammatory Response ◽  
Allergic Contact ◽  
G Protein Coupled

Abstract Imidazolidinyl urea (IU) is used as an antimicrobial preservative in cosmetic and pharmaceutical products. IU induces allergic contact dermatitis, however, the mechanism has not yet been elucidated. Mas-related G protein-coupled receptor-X2 (MRGPRX2) triggers drug-induced pseudo-allergic reactions. The aims of this study were to determine whether IU activated mast cells through MRGPRX2 to further trigger contact dermatitis. Wild-type (WT) and KitW-sh/HNihrJaeBsmJNju (MUT) mice were treated with IU to observe its effects on local inflammation and mast cells degranulation in vivo. Laboratory of allergic disease 2 cells were used to detect calcium mobilization and release of inflammatory mediators in vitro. WT mice showed a severe local inflammatory response and contact dermatitis, whereas only slight inflammatory infiltration was observed in MUT mice. Thus, MRGPRX2 mediated the IU-induced activation of mast cells. However, histamine, a typical allergen, was not involved in this process. Tryptase expressed by mast cells was the major non-histaminergic inflammatory mediator of contact dermatitis. IU induced anaphylactic reaction via MRGPRX2 and further triggering non-histaminergic contact dermatitis, which explained why antihistamines are clinically ineffective against some chronic dermatitis.

scholarly journals Allergic contact dermatitis: epidemiology, molecular mechanisms, in vitro methods and regulatory aspects

2011 ◽  
Vol 69 (5) ◽  
pp. 763-781 ◽  
Author(s):  
M. Peiser ◽  
T. Tralau ◽  
J. Heidler ◽  
A. M. Api ◽  
J. H. E. Arts ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
Molecular Mechanisms ◽  
In Vitro Methods ◽  
Regulatory Aspects ◽  
Allergic Contact
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