Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli

1984 ◽  
Vol 196 (3) ◽  
pp. 387-396 ◽  
Author(s):  
Egon B. Hansen ◽  
Tove Atlung ◽  
Flemming G. Hansen ◽  
Ole Skovgaard ◽  
Kaspar von Mevenburg
Keyword(s):  
Escherichia Coli ◽  
Fine Structure ◽  
Genetic Map ◽  
Complementation Analysis ◽  
Dnaa Gene

DELETION MAPPING OF zwf, THE GENE FOR A CONSTITUTIVE ENZYME, GLUCOSE 6-PHOSPHATE DEHYDROGENASE IN ESCHERICHIA COLI

Genetics ◽  
1972 ◽  
Vol 71 (4) ◽  
pp. 481-489
Author(s):  
D G Fraenkel ◽  
Santimoy Banerjee
Keyword(s):  
Escherichia Coli ◽  
Fine Structure ◽  
Mutant Strain ◽  
Genetic Map ◽  
Gene Order ◽  
Sugar Metabolism ◽  
Deletion Mapping ◽  
Secondary Mutation ◽  
E Coli ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT Genes for three enzymes of intermediary sugar metabolism in E. coli, zwf (glucose 6-phosphate dehydrogenase, constitutive), edd (gluconate 6-phosphate dehydrase, inducible), and eda (2-keto-3-deoxygluconate 6-phosphate aldolase, differently inducible) are closely linked on the E. coli genetic map, the overall gene order being man… old… eda. edd. zwf… cheB… uvrC… his. One class of apparent revertants of an eda mutant strain contains a secondary mutation in edd, and some of these mutations are deletions extending into zwf. We have used a series of spontaneous edd-zwf deletions to map a series of point mutants in zwf and thus report the first fine structure map of a gene for a constitutive enzyme (zwf).

scholarly journals A genetic map of several mutations affecting the mucopeptide layer ofEscherichia coli

1972 ◽  
Vol 20 (1) ◽  
pp. 65-74 ◽  
Author(s):  
H. J. W. Wijsman
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Fine Structure ◽  
Cell Division ◽  
Genetic Map ◽  
Genetic Relationship ◽  
Ofescherichia Coli ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Temperature Sensitive Mutants

SUMMARYSeveral temperature-sensitive mutants ofEscherichia coliwere isolated which lyse at the restrictive temperature. Some of these possess a biochemically defined lesion in cell-wall mucopeptide synthesis. Three genes, termedmurC, EandF, have been localized between theaziandleumarkers. From transductional data a fine structure map was constructed of themurmutations, establishing the order of the genes. The genetic relationship between these cell wall genes and neighbouring genes involved in cell division is discussed.

scholarly journals Overexpression of the dnaA gene in Escherichia coli B/r: chromosome and minichromosome replication in the presence of rifampin.

1987 ◽  
Vol 169 (5) ◽  
pp. 1871-1877 ◽  
Author(s):  
O Pierucci ◽  
C E Helmstetter ◽  
M Rickert ◽  
M Weinberger ◽  
A C Leonard
Keyword(s):  
Escherichia Coli ◽  
Dnaa Gene ◽  
Escherichia Coli B

Genetic map of Escherichia coli strain C

1970 ◽  
Vol 107 (1) ◽  
pp. 1-31 ◽  
Author(s):  
Marianne Wiman ◽  
G. Bertani ◽  
Beatrice Kelly ◽  
I. Sasaki
Keyword(s):  
Escherichia Coli ◽  
Genetic Map ◽  
Coli Strain

scholarly journals Genetic Organization of the Vibrio harveyi dnaA Gene Region and Analysis of the Function of the V. harveyi DnaA Protein in Escherichia coli

2002 ◽  
Vol 184 (9) ◽  
pp. 2533-2538 ◽  
Author(s):  
Dvora Berenstein ◽  
Kirsten Olesen ◽  
Christian Speck ◽  
Ole Skovgaard
Keyword(s):  
Escherichia Coli ◽  
Promoter Region ◽  
Vibrio Harveyi ◽  
Gene Region ◽  
Chromosomal Dna ◽  
Dnaa Gene ◽  
E Coli ◽  
Dnaa Protein ◽  
Dam Methylase ◽  
And Function

ABSTRACT The Vibrionaceae family is distantly related to Enterobacteriaceae within the group of bacteria possessing the Dam methylase system. We have cloned, sequenced, and analyzed the dnaA gene region of Vibrio harveyi and found that although the organization of the V. harveyi dnaA region differs from that of Escherichia coli, the expression of both genes is autoregulated and ATP-DnaA binds cooperatively to ATP-DnaA boxes in the dnaA promoter region. The DnaA proteins of V. harveyi and E. coli are interchangeable and function nearly identically in controlling dnaA transcription and the initiation of chromosomal DNA replication despite the evolutionary distance between these bacteria.

scholarly journals Genetic Fine Structure of the Leucine Operon of Escherichia coli K-12

1973 ◽  
Vol 113 (3) ◽  
pp. 1268-1272 ◽  
Author(s):  
J. M. Somers ◽  
A. Amzallag ◽  
R. B. Middleton
Keyword(s):  
Escherichia Coli ◽  
Fine Structure ◽  
Genetic Fine Structure ◽  
K 12
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