Chromosomal location of genes coding for endosperm proteins of Hordeum chilense, determined by two-dimensional electrophoresis of wheat-H. chilense chromosome addition lines

1987 ◽  
Vol 25 (1-2) ◽  
pp. 53-65 ◽  
Author(s):  
P. I. Payne ◽  
L. M. Holt ◽  
S. M. Reader ◽  
T. E. Miller
Keyword(s):  
Chromosomal Location ◽  
Hordeum Chilense ◽  
Two Dimensional ◽  
Addition Lines ◽  
Endosperm Proteins ◽  
Two Dimensional Electrophoresis ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Dimensional Electrophoresis

scholarly journals Identification of a second locus encoding β-amylase on chromosome 2 of barley

1988 ◽  
Vol 51 (1) ◽  
pp. 13-16 ◽  
Author(s):  
M. Kreis ◽  
M. S. Williamson ◽  
P. R. Shewry ◽  
P. Sharp ◽  
M. Gale
Keyword(s):  
Copy Number ◽  
Chromosomal Location ◽  
The Other ◽  
Chromosome 2 ◽  
Addition Lines ◽  
Chromosome 4 ◽  
Telocentric Chromosome ◽  
Chromosome Addition ◽  
Gene Copies ◽  
Chromosome Addition Lines

SummaryA barley endosperm cDNA clone was used to study the polymorphism and chromosomal location of β-amylase genes in barley. Analysis of DNA from seven cultivars digested with three restriction endonucleases showed two types of pattern, one present in Sultan and the other in the remaining six cultivars. A copy-number reconstruction indicated the presence of about three gene copies per haploid genome. Analysis of the six available whole chromosome addition lines and selected telocentric chromosome additions of barley into wheat showed the location of genes on the short arm of chromosome 2 (probably one copy) and the long arm of chromosome 4 (probably two copies).

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativaL.) Proteomics

2012 ◽  
Vol 18 (5) ◽  
pp. 819 ◽  
Author(s):  
Yanhua YANG ◽  
Weitong CUI ◽  
Xiaoyong LIU ◽  
Keming ZHU ◽  
Keping CHEN
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Establishment of Sorangium cellulosum So0157-2 Proteome Database Using Optimized Two-dimensional Electrophoresis Protocol*

2012 ◽  
Vol 39 (1) ◽  
pp. 86-94
Author(s):  
Peng-Yi ZHANG ◽  
Yue-Zhong LI ◽  
Zhi-Hong WU ◽  
Hong LIU ◽  
Pei-Pei XU ◽  
...  
Keyword(s):  
Two Dimensional ◽  
Sorangium Cellulosum ◽  
Two Dimensional Electrophoresis ◽  
Proteome Database ◽  
Dimensional Electrophoresis

Establishment of two-dimensional electrophoresis(2-DE)technique in muscle proteome of Fenneropenaeus chinensis

2013 ◽  
Vol 37 (2) ◽  
pp. 288
Author(s):  
Zhiyuan LIU ◽  
Jianrong LI ◽  
Xuepeng LI ◽  
Tingting LI ◽  
Yanbo WANG ◽  
...  
Keyword(s):  
Fenneropenaeus Chinensis ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Muscle Proteome ◽  
Dimensional Electrophoresis

scholarly journals Production and Characterization of Maize Chromosome 9 Radiation Hybrids Derived From an Oat-Maize Addition Line

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 327-339 ◽  
Author(s):  
O Riera-Lizarazu ◽  
M I Vales ◽  
E V Ananiev ◽  
H W Rines ◽  
R L Phillips
Keyword(s):  
Radiation Hybrid ◽  
Chromosome Region ◽  
Chromosome 9 ◽  
Addition Line ◽  
Addition Lines ◽  
Organization Studies ◽  
Maize Chromosome ◽  
Chromosome Addition ◽  
Radiation Hybrids ◽  
Chromosome Addition Lines

Abstract In maize (Zea mays L., 2n = 2x = 20), map-based cloning and genome organization studies are often complicated because of the complexity of the genome. Maize chromosome addition lines of hexaploid cultivated oat (Avena sativa L., 2n = 6x = 42), where maize chromosomes can be individually manipulated, represent unique materials for maize genome analysis. Maize chromosome addition lines are particularly suitable for the dissection of a single maize chromosome using radiation because cultivated oat is an allohexaploid in which multiple copies of the oat basic genome provide buffering to chromosomal aberrations and other mutations. Irradiation (gamma rays at 30, 40, and 50 krad) of a monosomic maize chromosome 9 addition line produced maize chromosome 9 radiation hybrids (M9RHs)—oat lines possessing different fragments of maize chromosome 9 including intergenomic translocations and modified maize addition chromosomes with internal and terminal deletions. M9RHs with 1 to 10 radiation-induced breaks per chromosome were identified. We estimated that a panel of 100 informative M9RHs (with an average of 3 breaks per chromosome) would allow mapping at the 0.5- to 1.0-Mb level of resolution. Because mapping with maize chromosome addition lines and radiation hybrid derivatives involves assays for the presence or absence of a given marker, monomorphic markers can be quickly and efficiently mapped to a chromosome region. Radiation hybrid derivatives also represent sources of region-specific DNA for cloning of genes or DNA markers.

Sign in / Sign up

Export Citation Format

Share Document