scholarly journals Carotenoid pigments fromOrobanche owerinii

1985 ◽  
Vol 21 (5) ◽  
pp. 672-672 ◽  
Author(s):  
S. F. Dzhumyrko ◽  
N. V. Sergeeva
Keyword(s):  
1979 ◽  
Vol 149 (1) ◽  
pp. 27-39 ◽  
Author(s):  
H Rosen ◽  
S J Klebanoff

The acetaldehyde-xanthine oxidase system in the presence and absence of myeloperoxidase (MPO) and chloride has been employed as a model of the oxygen-dependent antimicrobial systems of the PMN. The unsupplemented xanthine oxidase system was bactericidal at relatively high acetaldehyde concentrations. The bactericidal activity was inhibited by superoxide dismutase (SOD), catalase, the hydroxyl radical (OH.) scavengers, mannitol and benzoate, the singlet oxygen (1O2) quenchers, azide, histidine, and 1,4-diazabicyclo[2,2,2]octane (DABCO) and by the purines, xanthine, hypoxanthine, and uric acid. The latter effect may account for the relatively weak bactericidal activity of the xanthine oxidase system when purines are employed as substrate. A white, carotenoid-negative mutant strain of Sarcina lutea was more susceptible to the acetaldehyde-xanthine oxidase system than was the yellow, carotenoid-positive parent strain. Carotenoid pigments are potent 1O2 quenchers. The xanthine oxidase system catalyzes the conversion of 2,5-diphenylfuran to cis-dibenzoylethylene, a reaction which can occur by a 1O2 mechanism. This conversion is inhibited by SOD, catalase, azide, histidine, DABCO, xanthine, hypoxanthine, and uric acid but is only slightly inhibited by mannitol and benzoate. The addition of MPO and chloride to the acetaldehyde-xanthine oxidase system greatly increases bactericidal activity; the minimal effective acetaldehyde concentration is decreased 100-fold and the rate and extent of bacterial killing is increased. The bactericidal activity of the MPO-supplemented system is inhibited by catalase, benzoate, azide, DABCO, and histidine but not by SOD or mannitol. Thus, the acetaldehyde-xanthine oxidase system which like phagocytosing PMNs generates superoxide (O.2-) and hydrogen peroxide, is bactericidal both in the presence and absence of MPO and chloride. The MPO-supplemented system is considerably more potent; however, when MPO is absent, bactericidal activity is observed which may be mediated by the interaction of H2O2 and O.2- to form OH. and 1O2.


1982 ◽  
Vol 18 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Jeana Gross
Keyword(s):  

1944 ◽  
Vol 9 (6) ◽  
pp. 427-433 ◽  
Author(s):  
S. LASSEN ◽  
K. BACON ◽  
J. SUTHERLAND

2015 ◽  
Vol 8 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Jana Tkáčová ◽  
Katarína Furdíková ◽  
Tatiana Klempová ◽  
Katarína Ďurčanská ◽  
Milan Čertík

Abstract Carotenoids represent large group of various natural pigments ensuring typical coloration of plants, microorganisms and several animals. It was confirmed by many studies, that consuming these biological active compounds has positive impact for human life. Therefore, they are applied in different industrial fields, such as pharmacy, cosmetic, food, and feed industry. Due to high demand for carotenoids we would like to discover new microorganisms overproducing carotenoids. We focused on yeasts of genus Rhodotorula sp. (forty isolates), that we screened according to growth and carotenoid production on Petri dishes and production media. After cultivation on Petri dishes we selected five strains (denoted as KF-4, KF-6, KF-24, KF-31, KF-104) with interesting pigment production and quick growth. The secondary screening on production media identified KF-104 as the best producer of carotenoid pigments with massive pigment accumulation (1.15 mg/g DCW) and yield (9.69 mg/L). The main carotenoid of KF-104 isolate was β-carotene (35.4 %) with the accumulation of 408.7 μg/g DCW and the yield of 3.4 mg/L. The rest were torularhodin, torulene and γ-carotene (62.7–79.0 %). Production of torularhodin in the cells was low (0.4 to 1.4 mg/L) as was its accumulation in cells (31.2–121.0 μg/g DCW). We continue the experimental analyses of these isolates in order understand differences in the content of individual pigments.


1987 ◽  
Vol 56 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Keiichi WATANABE ◽  
Tadao SAITO ◽  
Saishi HIROTA ◽  
Bunjiro TAKAHASHI
Keyword(s):  

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