Detection of microorganisms with antibacterial activities from different industrial wastes and GC-MS analysis of crude microbial extract

Many soil microorganisms’ i.e., bacteria and fungi produce secondary metabolites called antibiotics. These are used for the treatment of some of the bacterial, fungal and protozoal diseases of humans. There is a need for isolation of a broad spectrum of antibiotics from microorganisms due to the emergence of antibiotic resistance. In the present study two antibiotic producing bacteria Klebsiella pneumoniae and Bacillus cereus were isolated from pharmaceutical and poultry feed industry of Hattar, Haripur Pakistan. Total 10 waste samples were collected from different industries (Marble, Ghee, Soap, Mineral, Steel, Poultry Feed, Pharmaceutical, Qarshi, Cosmetic and Glass). Thirty-three bacterial strains were isolated from industrial wastes of these ten different industries. Fourteen out of thirty-three bacterial strains exhibited antimicrobial activities against at least one of the test microbes considered in this study including Escherchia coli, Staphylococcus aureus and Salmonella typhi. The bacteria were isolated by standard serial dilution spread plate technique. Morphological characterization of the isolates was done by Gram staining. Nine bacterial isolates out of fourteen were initially identified as B. cereus and five as K. pneumoniae through biochemical characterization. The antibacterial activities were tested by well diffusion method. Maximum number of antibiotic producing bacteria were isolated from pharmaceutical and poultry feed industry based on the results of primary screening, the most potential isolates S9, S19, S20, S22 and S23 were selected for secondary screening. The maximum activity against E. coli and S. aureus was recorded by bacterial isolate S19 i.e zones of inhibition of 6.5mm and 9mm while S20 showed 7.5mm and 6mm zones respectively. Molecular identification was carried out on the basis of 16S rRNA sequence analysis. Finally, the isolates were identified as B. cereus accession number LC538271and K. pneumoniae accession number MT078679. Analysis of bacterial extract S20 through GC-MS indicated the presence of 8 compounds of diverse nature and structure. Present study suggests that wastes of pharmaceutical and poultry feed industry may have antibiotic producing bacteria. These bacteria could be utilized for the production of antibiotics. B. cereus and K. pneumoniae isolated from wastes of poultry feed and pharmaceutical industries have the potential to produce antibiotics and could be used to control the microbial growth.

Responsibility and Sustainability Choices in the Animal Feed Industry

The conventional discourse on corporate social responsibility (CSR) focuses on big companies and tends to neglect small, medium, and family firms. However, scholars state that simply scaling down CSR theories does not capture the variations in CSR choices across companies and contexts. The authors remedy this state of affairs by investigating an Italian family firm in the animal feed industry in light of an integrative framework that combines institutional- and company-level factors explaining the variations in CSR choices. The findings highlight how the company under investigation is committed to ensuring animal welfare by offering healthy and safe animal feed through innovation and certification. In addition, the company is well embedded in the local community and represents a point of reference for the inhabitants. Initiatives ranging from scholarships to university exchange programs to running races contribute to mobilizing human resources and to improving the company's brand awareness.

Development of production technology for extruded starter compound feedsfor juvenile fish

The article presents the results of developing recipes for domestic extruded starter compound feeds for zander, tilapia and Clary catfish. Technological modes of their production, as well as physical-chemical, mechanical and technological indicators of the developed compound feeds are established. The developed compound feeds are well-loose grains of dark and light brown colors, with sizes from 0,2 to 2,0 mm. Indicators of water resistance were at least 2,5 hours, crumbling no more than 1,5 %, swelling of granules 10-15 minutes, volume mass below 1000 kg/m3. The data obtained show that the developed feed meets the regulatory requirements for starting fish feeds. The introduction of the developed technology for the production of starter compound feeds at domestic enterprises of the feed industry will reduce the dependence of industrial fish farms on the import of compound feeds and thereby increase the profitability of the industry.

Study of microflora change and preservation of vitamins b2 and e of grain in the process of steaming and flaking

The study of the process of moisture-thermal treatment of grain crops: wheat, oats, barley, corn, peas, bran and others was carried out in the work. The drying process examination was done on a drying plant located in the testing laboratory of JSC "Research and Production Center" All-Russian Research Institute of the Feed Industry ". Studies of the grain qualitative characteristics were carried out in the accredited testing laboratory of JSC "RPC" ARSRIFI", which allows carrying physical- and chemical analysis, mycotoxicology and microbiology of plant raw materials.

PREVENTION AND DETOXIFICATION OF MYCOTOXINS IN FEED

Through a comprehensive review of published reports on mycotoxin removal strategies, this work aims to update the understanding of the removal of mycotoxins present in the feed. Mycotoxins in feed: from prevention in the field to detoxification by adsorption or transformation. Mycotoxins are secondary metabolites present worldwide in agricultural products and produced by fungi that cause a toxic response (mycotoxicosis) when ingested by animals. Prevention of mycotoxicosis includes strategies before and after harvesting. The best way to reduce the content of mycotoxins in the feed is to prevent the formation of mycotoxins, but it is often not enough, so other methods are needed. The most common approach in the feed industry is the inclusion of sorbing materials in the feed, which provides more or less selective removal of toxins by adsorption during passage through the gastrointestinal tract. Another reliable approach is the addition of enzymes or microorganisms that can remove toxins from some mycotoxins.

Strategies for sustainable corn production: a case of South Lampung District, Lampung Province, Indonesia

Demand for corn has been increasing from time to time. However, efforts to increase its production face multidimensional challenges and problems. This study aims to assess sustainability status, analyze leverage and prospective factors, and formulate follow-up strategies for sustainable corn production. Data used primarily were data collected through online focus group discussions and interviews with various related agencies and key informants at provincial and district levels. This study was conducted between May and October 2020 in South Lampung District. Multidimensional Scaling (MDS) and Matrix of Cross Impact Multiplications Applied to Classification (MICMAC) were used to assess the sustainability status and analyze leverage and prospective factors. Then, the Multicriteria Policy (MULTIPOL) was used to formulate the follow-up strategies. The results show that the corn production in South Lampung District is classified as less sustainable, with a sustainability index of 49.30. The sustainability of corn production is influenced by ten leverage attributes, four of which are prospective factors, namely corn prices at the farmer level, the number of water pumps, the use of natural pesticides and fertilizers, and the number of corn shellers. The strategies for sustainable corn production could be carried out by by applying reference purchase prices effectively, strengthening farmer partnerships with the feed industry, supporting environmentally friendly corn farming, and optimization and effective use of pre-harvest and post-harvest machinery. It is necessary to evaluate the effectiveness of various assistance and policies implemented at this time to improve the implementation of programs/policies in the future.

84 The Adoption of AI in the Core Scientific Cycle of Feed Research

The Gartner Hype Cycle methodology highlights five self-explanatory steps through which a technological innovation needs to go: (1) innovation trigger, (2) peak of inflated expectations, (3) trough of disillusionment, (4) slope of enlightenment, and (5) plateau of productivity. It is between step 4 and 5 that any tech innovation really starts to commercially pay off. For Artificial Intelligence (AI), the possibilities and challenges are so diverse that several separate cycles now exist for distinct parts of AI development. For the Animal Sciences / Feed industry the application of AI is not straightforward. In fact, in most industries, application is not straightforward because applications equals implementation. Hence, being able to translate the pains and gains of your customer into the models that you create, and finding a way to implement it, is key and that is more than just applying AI. The majority of (animal) models in the feed industry are mechanistic by nature. Parameters are generated/updated via controlled experiments, and stochasticity is allowed via Monte Carlo simulations and scenario analyses. The validity of the model is its ability to provide usable growth/health predictions, enable least cost formulation, and provide a sustainability footprint. To further support such models, and to offer new services, we have recently begun to combine our near-infrared spectroscopy(NIR), laboratory information management system (LIMS) and mycotoxin databases with climate and geographic data to (1) predict the nutrient composition of raw materials over time, (2) enable risk assessment of mycotoxin co-contamination, (3) improve the feed evaluation of silages, (4) estimate a full nutrient profile, and (5) improve the precision of net energy estimation. In the end, if nurtured carefully, AI is just another technical leap that needs to be integrated into the core scientific cycle.

Evolution of the mineral concentration and bioaccumulation of the black soldier fly, Hermetia illucens, feeding on two different larval media

Intensive black soldier fly (BSF) Hermetia illucens (Diptera: Stratiomyidae) rearing has a great importance for the feed industry. The objective of the present work was to perform a systematic study about the effect of the feeding media on the concentration and bioaccumulation factor (BAF) of Ca, K, Mg, Na, Cr, Cu, Fe, Mn, Ni and Zn in BSF fractions. Therefore, the evolution of the concentration of these elements has been studied along the stages of the BSF life cycle (larva, pupa and adult) as well as during its growth (exuviae) and metamorphosis (empty puparia). Two diets consisting of beer bagasse and commercial hen feed moistened with water have been chosen. An inductively coupled plasma optical emission spectrometer has been used to perform the elemental analyses. The obtained results demonstrated that the behaviour was generally, similar for all the elements studied, i.e. an accumulation in exuviae and puparia followed by their further elimination. Moreover, no significant differences in the results as a function of the feeding media have been found. This may be attributed to the fact that the chosen diets were equally favourable for this insect’s growth. The obtained concentrations have been expressed on a dry as well as a wet sample basis. BAF results also suggested that exuviae and puparia could be used as supplements or as bioactive compounds.

Plant Feed Additives as Natural Alternatives to the Use of Synthetic Antioxidant Vitamins on Livestock Mammals’ Performances, Health, and Oxidative Status: A Review of the Literature in the Last 20 Years

In the last two decades, the interest in natural plant feed additives (PFA) as alternatives to synthetic vitamins in livestock nutrition has increased. After a systematic review, a total of 19 peer-reviewed papers published between 2000 and 2020 were retained to evaluate the antioxidant effects of PFA compared to synthetic antioxidant vitamins (mainly vitamin E; VitE) in livestock nutrition. These studies demonstrated that PFAs could be as efficient as VitE in counteracting oxidative stress in pigs, rabbits, and ruminants. However, PFAs only positively affected animals’ growth performance and feed efficiency in some monogastric studies. The PFA can affect antioxidant enzyme activity in a dose- and method of administration-dependent manner. The antioxidant capacity of both PFA and VitE were depressed in cows fed with diets rich in polyunsaturated fatty acids. Variability among studies could be related to species differences. Despite the interest of the feed industry sector in PFA, there are still very few studies evaluating their antioxidant effect in species other than poultry.