Genetic mapping of regulatory mutations ofBacillus subtilis riboflavin operon

1990 ◽  
Vol 222 (2-3) ◽  
pp. 467-469 ◽  
Author(s):  
Rimma A. Kreneva ◽  
Daniel A. Perumov

1979 ◽  
Vol 139 (3) ◽  
pp. 859-865 ◽  
Author(s):  
J J Mekalanos ◽  
R D Sublett ◽  
W R Romig


Author(s):  
J. W. Van Ooijen ◽  
J. Jansen


2011 ◽  
Vol 37 (10) ◽  
pp. 1779-1784
Author(s):  
Ming-Jing ZHOU ◽  
Yong WEN ◽  
Shuang-Cheng LI ◽  
Cheng-Bo LI ◽  
Man-Hua ZHANG ◽  
...  


2009 ◽  
Vol 295 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Monica Gupta ◽  
Kestur Krishnamurthy Rao


Genetics ◽  
1987 ◽  
Vol 116 (4) ◽  
pp. 513-521
Author(s):  
Nancy J Trun ◽  
Thomas J Silhavy

ABSTRACT The prlC gene of E. coli was originally identified as an allele, prlC1, which suppresses certain signal sequence mutations in the genes for several exported proteins. We have isolated six new alleles of prlC that also confer this phenotype. These mutations can be placed into three classes based on the degree to which they suppress the lamBsignal sequence deletion, lamBs78. Genetic mapping reveals that the physical location of the mutations in prlC correlates with the strength of the suppression, suggesting that different regions of the gene can be altered to yield a suppressor phenotype. We also describe an in vivo cloning procedure using λplacMu9H. The procedure relies on transposition and illegitimate recombination to generate a specialized transducing phage that carries prlC1. This method should be applicable to any gene for which there is a mutant phenotype.



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