Effect of monoclonal antibodies to bovine nerve growth factor (NGF) on NGF-induced cell differentiation in vitro

1989 ◽  
Vol 108 (5) ◽  
pp. 1648-1650
Author(s):  
E. S. Kakpakova ◽  
E. M. Malakhova ◽  
S. N. Gnedoi ◽  
T. V. Pochechueva ◽  
I. A. Filatov ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Differentiation ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth

scholarly journals The Mixture of Gotu Kola, Cnidium Fruit, and Goji Berry Enhances Memory Functions by Inducing Nerve-Growth-Factor-Mediated Actions Both In Vitro and In Vivo

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1372
Author(s):  
Jin Gyu Choi ◽  
Zahra Khan ◽  
Seong Min Hong ◽  
Young Choong Kim ◽  
Myung Sook Oh ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neuronal Cell ◽  
Main Role ◽  
Memory Functions ◽  
Nerve Growth ◽  
Goji Berry

Nerve growth factor (NGF), a typical neurotrophin, has been characterized by the regulation of neuronal cell differentiation and survival involved in learning and memory functions. NGF has a main role in neurite extension and synapse formation by activating the cyclic adenosine monophosphate-response-element-binding protein (CREB) in the hippocampus. The purpose of this study was to determine whether a mixture of Gotu Kola, Cnidium fruit, and Goji berry (KYJ) enhances memory function by inducing NGF-mediated actions both in vitro and in vivo. The KYJ combination increased NGF concentration and neurite length in C6 glioma and N2a neuronal cells, respectively. Additionally, we discovered memory-enhancing effects of KYJ through increased NGF-mediated synapse maturation, CREB phosphorylation, and cell differentiation in the mouse hippocampus. These findings suggest that this combination may be a potential nootropic cognitive enhancer via the induction of NGF and NGF-dependent activities.

In Vitro Evidence of Nerve Growth Factor Effects on Human Conjunctival Epithelial Cell Differentiation and Mucin Gene Expression

2009 ◽  
Vol 50 (10) ◽  
pp. 4622 ◽  
Author(s):  
Alessandro Lambiase ◽  
Alessandra Micera ◽  
Graziella Pellegrini ◽  
Daniela Merlo ◽  
Paolo Rama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Differentiation ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Epithelial Cell ◽  
Nerve Growth ◽  
Epithelial Cell Differentiation ◽  
Mucin Gene ◽  
Conjunctival Epithelial Cell

Proangiogene und Tumor fördernde Eigenschaften des Nerve Growth Factor auf murine Lungenkarzinome in vitro

Pneumologie ◽  
2010 ◽  
Vol 64 (01) ◽  
Author(s):  
KM Seidler ◽  
H Renz ◽  
WA Nockher
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth

Neuroprotective and anti-amnestic effects of a combination therapy in a model of photochemical ischemic damage in the prefrontal cortex

2018 ◽  
pp. 39-45
Author(s):  
Ф.М. Шакова ◽  
Т.И. Калинина ◽  
М.В. Гуляев ◽  
Г.А. Романова
Keyword(s):  
Prefrontal Cortex ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Combination Therapy ◽  
Neuroprotective Effect ◽  
Regulatory Protein ◽  
Human Growth ◽  
Neuroprotective Effects ◽  
Nerve Growth

Цель исследования - изучение влияния комбинированной терапии (мутантные молекулы эритропоэтина (EPO) и дипептидный миметик фактора роста нервов ГК-2H) на воспроизведение условного рефлекса пассивного избегания (УРПИ) и объем поражения коры мозга у крыс с двусторонним ишемическим повреждением префронтальной коры. Методика. Мутантные молекулы EPO (MЕРО-TR и MЕPО-Fc) с значительно редуцированной эритропоэтической и выраженной цитопротекторной активностью созданы методом генной инженерии. Используемый миметик фактора роста нервов человека, эндогенного регуляторного белка, в экспериментах in vitro проявлял отчетливые нейропротективные свойства. Двустороннюю фокальную ишемию префронтальной коры головного мозга крыс создавали методом фотохимического тромбоза. Выработку и оценку УРПИ проводили по стандартной методике. Объем повреждения мозга оценивался при помощи МРТ. MEPO-TR и MEPO-Fc (50 мкг/кг) вводили интраназально однократно через 1 ч после фототромбоза, ГК-2Н (1 мг/кг) - внутрибрюшинно через 4 ч после фототромбоза и далее в течение 4 послеоперационных суток. Результаты. Выявлено статистически значимое сохранение выработанного до ишемии УРПИ, а также значимое снижение объема повреждения коры при комплексной терапии. Полученные данные свидетельствуют об антиамнестическом и нейропротекторном эффектах примененной комбинированной терапии, которые наиболее отчетливо выражены в дозах: МEPO-Fc (50 мкг/кг) и ГК-2Н (1 мг/кг). Заключение. Подтвержден нейропротекторный эффект и усиление антиамнестического эффекта при сочетанном применении мутантных производных эритропоэтина - MEPO-TR и MEPO-Fc и дипептидного миметика фактора роста нервов человека ГК-2H. The aim of this study was to investigate the effect of combination therapy, including mutant erythropoietin molecules (EPO) and a dipeptide mimetic of the nerve growth factor, GK-2H, on the conditioned passive avoidance (PA) reflex and the volume of injury induced by bilateral ischemia of the prefrontal cortex in rats. Using the method of genetic engineering the mutant molecules of EPO, MERO-TR and MEPO-Fc, with strongly reduced erythropoietic and pronounced cytoprotective activity were created. The used human nerve growth factor mimetic, an endogenous regulatory protein based on the b-bend of loop 4, which is a dimeric substituted dipeptide of bis- (N-monosuccinyl-glycyl-lysine) hexamethylenediamine, GK-2 human (GK-2H), has proven neuroprotective in in vitro experiments. Methods. Bilateral focal ischemic infarction was modeled in the rat prefrontal cortex by photochemically induced thrombosis. The PA test was performed according to a standard method. Volume of brain injury was estimated using MRI. MEPO-TR, and MEPO-Fc (50 mg/kg, intranasally) were administered once, one hour after the injury. GK-2Н (1 mg/kg, i.p.) was injected four hours after the injury and then for next four days. Results. The study showed that the complex therapy provided statistically significant retention of the PA reflex developed prior to ischemia and a significant decrease in the volume of injury. The anti-amnestic and neuroprotective effects of combination therapy were most pronounced at doses of MEPO-Fc 50 mg/kg and GK-2H 1 mg/kg. Conclusion. This study has confirmed the neuroprotective effect and enhancement of the anti-amnestic effect exerted by the combination of mutant erythropoietin derivatives, MEPO-TR and MEPO-Fc, and the dipeptide mimetic of human growth factor GK-2H.

scholarly journals Nerve growth factor-mediated induction of tyrosine hydroxylase in rat superior cervical ganglia in vitro.

1978 ◽  
Vol 253 (22) ◽  
pp. 8013-8015
Author(s):  
S.R. Max ◽  
H. Rohrer ◽  
U. Otten ◽  
H. Thoenen
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Tyrosine Hydroxylase ◽  
Superior Cervical Ganglia ◽  
Nerve Growth ◽  
Cervical Ganglia

scholarly journals Gangliosides potentiate in vivo and in vitro effects of nerve growth factor on central cholinergic neurons.

1989 ◽  
Vol 86 (6) ◽  
pp. 2056-2060 ◽  
Author(s):  
A. C. Cuello ◽  
L. Garofalo ◽  
R. L. Kenigsberg ◽  
D. Maysinger
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cholinergic Neurons ◽  
Nerve Growth ◽  
In Vitro Effects

Nerve growth factor promotes development of the rat septo-hippocampal cholinergic projection in vitro

1987 ◽  
Vol 75 (1) ◽  
pp. 6-10 ◽  
Author(s):  
B.H. Gähwiler ◽  
A. Enz ◽  
F. Hefti
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Cholinergic Projection

scholarly journals Cartilage injury regulates nerve growth factor (NGF) in vitro and in vivo and drives painful behaviour in murine OA

2014 ◽  
Vol 22 ◽  
pp. S35
Author(s):  
C. Driscoll ◽  
A. Chanalaris ◽  
C. Knight ◽  
C. Gentry ◽  
S. Bevan ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cartilage Injury ◽  
Nerve Growth

The effects of gradients of nerve growth factor immobilized PCLA scaffolds on neurite outgrowth in vitro and peripheral nerve regeneration in rats

Biomaterials ◽  
2013 ◽  
Vol 34 (29) ◽  
pp. 7086-7096 ◽  
Author(s):  
Shuo Tang ◽  
Jixiang Zhu ◽  
Yangbin Xu ◽  
Andy Peng Xiang ◽  
Mei Hua Jiang ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Peripheral Nerve ◽  
Neurite Outgrowth ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Growth
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