Probing the human B-cell repertoire: Isolation of Epstein-Barr virus (EBV)-transformed human B lymphocytes making antibodies with a common idiotope that have different antigen-binding specificities

1988 ◽  
Vol 8 (6) ◽  
pp. 459-463
Author(s):  
Yasuko Uchigata ◽  
Bellur S. Prabhakar ◽  
Abner Louis Notkins
Keyword(s):  
B Cell ◽  
B Lymphocytes ◽  
Epstein Barr Virus ◽  
Antigen Binding ◽  
Cell Repertoire ◽  
Barr Virus ◽  
B Cell Repertoire ◽  
Epstein Barr ◽  
Human B Cell

scholarly journals Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10.

1993 ◽  
Vol 177 (2) ◽  
pp. 295-304 ◽  
Author(s):  
N Burdin ◽  
C Péronne ◽  
J Banchereau ◽  
F Rousset
Keyword(s):  
Cell Proliferation ◽  
B Cell ◽  
B Lymphocytes ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Interleukin 10 ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Human B Cell

Interleukin 10 (IL-10) is a pleiotropic factor that enhances proliferation of activated human B lymphocytes and induces them to secrete high amounts of immunoglobulins. Here we show that several human B cell lines were able to constitutively secrete human (h)IL-10. Whereas none of the pre-B nor the plasmocytic cell lines tested produced hIL-10, 25 of the 36 tested mature B cell lines (lymphoblastoid and Burkitt lymphoma cell lines) secreted hIL-10. Moreover, 24 of these 25 hIL-10-producing B cell lines contained the Epstein-Barr virus (EBV) genome, suggesting a relationship between hIL-10 production by human B cell lines and EBV expression. Accordingly, whereas polyclonal activation via triggering of surface immunoglobulins or CD40 antigen induced highly purified normal human B lymphocytes to produce only low (0.3-0.4 ng/ml) but significant amounts of hIL-10, EBV infection induced them to secrete high amounts of hIL-10 (4-9 ng/ml). Furthermore, addition of exogenous hIL-10, simultaneously to EBV infection, potentiated cell proliferation, whereas a blocking anti-IL-10 antiserum inhibited it. Thus, hIL-10 produced by infected human B lymphocytes appears to be involved in the mechanisms of EBV-induced B cell proliferation.

Functional Analysis of an Epstein-Barr Virus-Nontransformed Human B Cell Line

1988 ◽  
Vol 80 (3) ◽  
pp. 139-144 ◽  
Author(s):  
Y. Yasuda ◽  
T. Iwasaki ◽  
T. Hamano ◽  
K. Nagai
Keyword(s):  
Functional Analysis ◽  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell

scholarly journals Epstein-Barr virus transformed B lymphocytes produce interleukin-5

Blood ◽  
1990 ◽  
Vol 75 (7) ◽  
pp. 1400-1403 ◽  
Author(s):  
CC Paul ◽  
JR Keller ◽  
JM Armpriester ◽  
MA Baumann
Keyword(s):  
B Cell ◽  
B Lymphocytes ◽  
Messenger Rna ◽  
Epstein Barr Virus ◽  
Northern Analysis ◽  
Interleukin 5 ◽  
Barr Virus ◽  
Epstein Barr ◽  
Semi Solid ◽  
Culture Supernatants

Abstract Interleukin-5 (IL-5) has previously been isolated only as a product of T lymphocytes. We have found that Epstein-Barr virus transformed B lymphocytes produce large amounts of IL-5 activity in culture supernatants, inducing proliferation of murine BCL1 cells, and supporting the selective growth of eosinophil colonies in semi-solid culture. Production of IL-5 messenger RNA by transformed B-cell lines was verified by Northern analysis using a 3.2-kilobase cloned DNA fragment containing the full-length human IL-5 gene, and immunoreactive IL-5 was detected in B-cell culture supernatants. These findings suggest a possible expanded role for the B cell in the induction of eosinophilia, and should serve as a focus for additional investigation into possible roles for IL-5 in human B-cell proliferation and differentiation.

scholarly journals Epstein–Barr Virus-Induced Metabolic Rearrangements in Human B-Cell Lymphomas

2018 ◽  
Vol 9 ◽  
Author(s):  
Pier P. Piccaluga ◽  
Alessandra Weber ◽  
Maria R. Ambrosio ◽  
Yonis Ahmed ◽  
Lorenzo Leoncini
Keyword(s):  
B Cell ◽  
Epstein Barr Virus ◽  
B Cell Lymphomas ◽  
Barr Virus ◽  
Epstein Barr ◽  
Human B Cell
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