Ascorbic acid phosphate ester and wound healing in rabbit corneal alkali burns: Epithelial basement membrane and stroma

Corneas of diabetic patients have abnormal healing and epithelial adhesion, which may be due to alterations of the corneal extracellular matrix (ECM) and basement membrane (BM). To identify such alterations, various ECM and BM components and integrin receptors were studied by immunofluorescence on sections of normal and diabetic human corneas. Age-matched corneas from 15 normal subjects, 10 diabetics without diabetic retinopathy (DR), and 12 diabetics with DR were used. In DR corneas, the composition of the central epithelial BM was markedly altered, compared to normal or non-DR diabetic corneas. In most cases the staining for entactin/nidogen and for chains of laminin-1 (α1β1γ1) and laminin-10 (α5β1γ1) was very weak, discontinuous, or absent over large areas. Other BM components displayed less frequent changes. The staining for α3β1 (VLA-3) laminin binding integrin was also weak and discontinuous in DR corneal epithelium. Components of stromal ECM remained unchanged even in DR corneas. Therefore, distinct changes were identified in the composition of the epithelial BM in DR corneas. They may be due to increased degradation or decreased synthesis of BM components and related integrins. These alterations may directly contribute to the epithelial adhesion and wound healing abnormalities found in diabetic corneas.

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Spontaneous Chronic Corneal Epithelial Defects in Dogs: A Review

Journal of the American Animal Hospital Association ◽

10.5326/0410158 ◽

2005 ◽

Vol 41 (3) ◽

pp. 158-165 ◽

Cited By ~ 34

Author(s):

Ellison Bentley

Keyword(s):

Wound Healing ◽

Basement Membrane ◽

Success Rate ◽

Treatment Options ◽

Middle Aged ◽

Delayed Wound Healing ◽

Corneal Epithelial ◽

Histopathological Findings ◽

Epithelial Basement Membrane ◽

Epithelial Basement

Spontaneous chronic corneal epithelial defects (SCCEDs) in dogs are typically found in middle-aged dogs of all breeds. These epithelial defects may be present for weeks to months, particularly if left untreated or if treated inappropriately. Typical histopathological findings include loss of the corneal epithelial basement membrane and formation of a superficial, acellular, hyalinized zone in the stroma. Together, these histological abnormalities lead to delayed wound healing and poor epithelial adhesion. Epithelial debridement, anterior stromal puncture, grid keratotomy, and superficial keratectomy are the most common treatment options applied to the defects. Procedures that address the stromal changes present generally have a higher success rate than epithelial debridement alone.

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Abnormal Deposition of Laminin and Type IV Collagen at Corneal Epithelial Basement Membrane During Wound Healing in Diabetic Rats

Japanese Journal of Ophthalmology ◽

10.1016/s0021-5155(99)00095-7 ◽

1999 ◽

Vol 43 (5) ◽

pp. 343-347 ◽

Cited By ~ 18

Author(s):

N Sato

Keyword(s):

Wound Healing ◽

Basement Membrane ◽

Type Iv Collagen ◽

Diabetic Rats ◽

Corneal Epithelial ◽

Epithelial Basement Membrane ◽

Type Iv ◽

Epithelial Basement

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Sloughing of corneal epithelium and wound healing complications associated with laser in situ keratomileusis in patients with epithelial basement membrane dystrophy

American Journal of Ophthalmology ◽

10.1016/s0002-9394(00)00504-3 ◽

2000 ◽

Vol 130 (3) ◽

pp. 297-303 ◽

Cited By ~ 47

Author(s):

K.Alexander Dastgheib ◽

Thomas E. Clinch ◽

Edward E. Manche ◽

Peter Hersh ◽

John Ramsey

Keyword(s):

Wound Healing ◽

Basement Membrane ◽

Corneal Epithelium ◽

Laser In Situ Keratomileusis ◽

Epithelial Basement Membrane ◽

Epithelial Basement

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Kalinin: A new epithelial basement membrane adhesion molecule

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100085198 ◽

1991 ◽

Vol 49 ◽

pp. 178-179

Author(s):

Douglas R. Keene ◽

Gregory P. Lunstrum ◽

Patricia Rousselle ◽

Robert E. Burgeson

Keyword(s):

Basement Membrane ◽

Culture Media ◽

Polyacrylamide Gel Electrophoresis ◽

Human Keratinocytes ◽

Positive Staining ◽

Human Amnion ◽

Epithelial Basement Membrane ◽

Type Vii Collagen ◽

Keratinocyte Cell ◽

Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

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