Effect of soil characteristics on sequential reduction and methane production in sixteen rice paddy soils from China, the Philippines, and Italy

1999 ◽  
Vol 47 (3) ◽  
pp. 269-295 ◽  
Author(s):  
H. Yao ◽  
R. Conrad ◽  
R. Wassmann ◽  
H. U. Neue
Keyword(s):  
Methane Production ◽  
Rice Paddy ◽  
Soil Characteristics ◽  
The Philippines ◽  
Paddy Soils ◽  
Rice Paddy Soils ◽  
Sequential Reduction

scholarly journals Fractionation of stable carbon isotopes during acetate consumption by methanogenic and sulfidogenic microbial communities in rice paddy soils and lake sediments

2021 ◽  
Vol 18 (24) ◽  
pp. 6533-6546
Author(s):  
Ralf Conrad ◽  
Pengfei Liu ◽  
Peter Claus
Keyword(s):  
Microbial Communities ◽  
Lake Sediments ◽  
Lake Sediment ◽  
Stable Carbon Isotopes ◽  
Rice Paddy ◽  
Enrichment Factors ◽  
The Philippines ◽  
Paddy Soils ◽  
Aceticlastic Methanogenesis ◽  
Rice Paddy Soils

Abstract. Acetate is an important intermediate during the degradation of organic matter in anoxic flooded soils and sediments. Acetate is disproportionated to CH4 and CO2 by methanogenic or is oxidized to CO2 by sulfate-reducing microorganisms. These reactions result in carbon isotope fractionation, depending on the microbial species and their particular carbon metabolism. To learn more about the magnitude of the isotopic enrichment factors (ε) involved, acetate conversion to CH4 and CO2 was measured in anoxic paddy soils from Vercelli (Italy) and the International Rice Research Institute (IRRI, the Philippines) and in anoxic lake sediments from the northeastern and the southwestern basins of Lake Fuchskuhle (Germany). Acetate consumption was measured using samples of paddy soil or lake sediment suspended in water or in phosphate buffer (pH 7.0), both in the absence and presence of sulfate (gypsum), and of methyl fluoride (CH3F), an inhibitor of aceticlastic methanogenesis. Under methanogenic conditions, values of εac for acetate consumption were always in a range of −21 ‰ to −17 ‰ but higher in the lake sediment from the southwestern basin (−11 ‰). Under sulfidogenic conditions εac values tended to be slightly lower (−26 ‰ to −19 ‰), especially when aceticlastic methanogenesis was inhibited. Again, εac in the lake sediment of the southwestern basin was higher (−18 ‰ to −14 ‰). Determination of εCH4 from the accumulation of 13C in CH4 resulted in much lower values (−37 ‰ to −27 ‰) than from the depletion of 13C in acetate (−21 ‰ to −17 ‰ ), especially when acetate degradation was measured in buffer suspensions. The microbial communities were characterized by sequencing the bacterial 16S rRNA (ribosomal ribonucleic acid) genes as well as the methanogenic mcrA and sulfidogenic dsrB genes. The microbial communities were quite different between lake sediments and paddy soils but were similar in the sediments of the two lake basins and in the soils from Vercelli and the IRRI, and they were similar after preincubation without and with addition of sulfate (gypsum). The different microbial compositions could hardly serve for the prediction of the magnitude of enrichment factors.

An Empirical Model of Soil Chemical Properties that Regulate Methane Production in Japanese Rice Paddy Soils

2007 ◽  
Vol 36 (6) ◽  
pp. 1920-1925 ◽  
Author(s):  
Weiguo Cheng ◽  
Kazuyuki Yagi ◽  
Hiroko Akiyama ◽  
Seiichi Nishimura ◽  
Shigeto Sudo ◽  
...  
Keyword(s):  
Empirical Model ◽  
Methane Production ◽  
Chemical Properties ◽  
Rice Paddy ◽  
Paddy Soils ◽  
Soil Chemical Properties ◽  
Rice Paddy Soils

scholarly journals Metatranscriptomic Evidence for Direct Interspecies Electron Transfer between Geobacter and Methanothrix Species in Methanogenic Rice Paddy Soils

2017 ◽  
Vol 83 (9) ◽  
Author(s):  
Dawn E. Holmes ◽  
Pravin M. Shrestha ◽  
David J. F. Walker ◽  
Yan Dang ◽  
Kelly P. Nevin ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Methane Production ◽  
Sequence Similarity ◽  
Rice Paddy ◽  
Geobacter Sulfurreducens ◽  
Paddy Soils ◽  
Content Type ◽  
Direct Interspecies Electron Transfer ◽  
Interspecies Electron Transfer ◽  
Rice Paddy Soils

ABSTRACT The possibility that Methanothrix (formerly Methanosaeta) and Geobacter species cooperate via direct interspecies electron transfer (DIET) in terrestrial methanogenic environments was investigated in rice paddy soils. Genes with high sequence similarity to the gene for the PilA pilin monomer of the electrically conductive pili (e-pili) of Geobacter sulfurreducens accounted for over half of the PilA gene sequences in metagenomic libraries and 42% of the mRNA transcripts in RNA sequencing (RNA-seq) libraries. This abundance of e-pilin genes and transcripts is significant because e-pili can serve as conduits for DIET. Most of the e-pilin genes and transcripts were affiliated with Geobacter species, but sequences most closely related to putative e-pilin genes from genera such as Desulfobacterium, Deferribacter, Geoalkalibacter, and Desulfobacula, were also detected. Approximately 17% of all metagenomic and metatranscriptomic bacterial sequences clustered with Geobacter species, and the finding that Geobacter spp. were actively transcribing growth-related genes indicated that they were metabolically active in the soils. Genes coding for e-pilin were among the most highly transcribed Geobacter genes. In addition, homologs of genes encoding OmcS, a c-type cytochrome associated with the e-pili of G. sulfurreducens and required for DIET, were also highly expressed in the soils. Methanothrix species in the soils highly expressed genes for enzymes involved in the reduction of carbon dioxide to methane. DIET is the only electron donor known to support CO2 reduction in Methanothrix. Thus, these results are consistent with a model in which Geobacter species were providing electrons to Methanothrix species for methane production through electrical connections of e-pili. IMPORTANCE Methanothrix species are some of the most important microbial contributors to global methane production, but surprisingly little is known about their physiology and ecology. The possibility that DIET is a source of electrons for Methanothrix in methanogenic rice paddy soils is important because it demonstrates that the contribution that Methanothrix makes to methane production in terrestrial environments may extend beyond the conversion of acetate to methane. Furthermore, defined coculture studies have suggested that when Methanothrix species receive some of their energy from DIET, they grow faster than when acetate is their sole energy source. Thus, Methanothrix growth and metabolism in methanogenic soils may be faster and more robust than generally considered. The results also suggest that the reason that Geobacter species are repeatedly found to be among the most metabolically active microorganisms in methanogenic soils is that they grow syntrophically in cooperation with Methanothrix spp., and possibly other methanogens, via DIET.

Abundance of Methanogens, Methanotrophic Bacteria, and Denitrifiers in Rice Paddy Soils

Wetlands ◽  
2013 ◽  
Vol 34 (2) ◽  
pp. 213-223 ◽  
Author(s):  
Juyoung Seo ◽  
Inyoung Jang ◽  
Gerhard Gebauer ◽  
Hojeong Kang
Keyword(s):  
Rice Paddy ◽  
Paddy Soils ◽  
Methanotrophic Bacteria ◽  
Rice Paddy Soils

scholarly journals Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China

2018 ◽  
Vol 20 (4) ◽  
pp. 673-685 ◽  
Author(s):  
Tatiana A. Vishnivetskaya ◽  
Haiyan Hu ◽  
Joy D. Van Nostrand ◽  
Ann M. Wymore ◽  
Xiaohang Xu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Gene Diversity ◽  
Rice Paddy ◽  
Paddy Soils ◽  
Rice Paddies ◽  
Sulfate Reducing ◽  
Rice Paddy Soils ◽  
Reducing Bacteria

Sulfate-reducing bacteria and methanogens are the primary Hg-methylators in Chinese rice paddies.

Abundance of rare earth elements in rice paddy soils from three regions of Sri Lanka

2004 ◽  
Vol 2 (3) ◽  
pp. 163-169 ◽  
Author(s):  
Rohana Chandrajith ◽  
Chandrasekara Bandara Dissanayake ◽  
Heinz J�rgen Tobschall
Keyword(s):  
Rare Earth ◽  
Sri Lanka ◽  
Rare Earth Elements ◽  
Rice Paddy ◽  
Paddy Soils ◽  
Rice Paddy Soils
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