Repeated application of first-layer antiserum improves immunofluorescence staining: a modification of the indirect immunofluorescence staining procedure

1983 ◽  
Vol 15 (5) ◽  
pp. 475-482 ◽  
Author(s):  
J. Gu ◽  
K. N. Islam ◽  
J. M. Polak
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Staining ◽  
Staining Procedure ◽  
Repeated Application ◽  
Indirect Immunofluorescence Staining

An indirect immunofluorescence staining procedure for detection of human Fcγ receptors on streptococci

1982 ◽  
Vol 48 (3) ◽  
pp. 349-358 ◽  
Author(s):  
Léa Lebrun ◽  
Jacques Pillot ◽  
Liliane Grangeot-Keros ◽  
Solange D'Azambuja
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Staining ◽  
Staining Procedure ◽  
Fcγ Receptors ◽  
Indirect Immunofluorescence Staining

scholarly journals Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Monika Danchenko ◽  
Lucia Csaderova ◽  
Pierre Edouard Fournier ◽  
Zuzana Sekeyova
Keyword(s):  
Indirect Immunofluorescence ◽  
Actin Filaments ◽  
Immunofluorescence Assay ◽  
Host Cells ◽  
Immunofluorescence Staining ◽  
Sensitive Detection ◽  
Filamentous Actin ◽  
Indirect Immunofluorescence Staining ◽  
Accurate Imaging

Abstract Objective The objective was to investigate fixative solutions: 3.7% formaldehyde, 4% paraformaldehyde, 4% paraformaldehyde in the cytoskeletal buffer and 4% paraformaldehyde in PHEM buffer (containing PIPES, HEPES, EGTA and MgCl2), applicable for immunofluorescence assay. Results Herein we optimized this serological technique, testing four fixative solutions, for the sensitive detection of rickettsial antigens, and preservation of intracellular structures of the host cells, particularly filamentous actin. Rickettsial antigens were presented equally well both with formaldehyde and all paraformaldehyde-based fixations, but only protocol with 4% paraformaldehyde in PHEM buffer allowed accurate imaging of actin filaments, and simultaneously allows monitoring of rickettsiae using actin-based motility during infection inside the host cells.

Indirect immunofluorescence staining and crossed immunoelectrophoresis for differentiation ofCandida albicansandGeotrichum candidum

Mycoses ◽  
1990 ◽  
Vol 33 (11-12) ◽  
pp. 519-526 ◽  
Author(s):  
H. E. Jensen ◽  
J. Hau ◽  
B. Aalbaek ◽  
H. Schønheyder
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Staining ◽  
Crossed Immunoelectrophoresis ◽  
Indirect Immunofluorescence Staining

Expression of neutral glycosphingolipids and gangliosides in human skeletal and heart muscle determined by indirect immunofluorescence staining

1994 ◽  
Vol 11 (5) ◽  
pp. 477-485 ◽  
Author(s):  
Melita Čačić ◽  
Johannes Müthing ◽  
Ivan Kračun ◽  
Ulrich Neumann ◽  
Sabine Weber-Schürholz
Keyword(s):  
Indirect Immunofluorescence ◽  
Heart Muscle ◽  
Immunofluorescence Staining ◽  
Neutral Glycosphingolipids ◽  
Indirect Immunofluorescence Staining

SEM study of cytoskeleton of osteoblasts immobilized on HA-ceramic during biocompatibility testing

1992 ◽  
Vol 50 (1) ◽  
pp. 784-785
Author(s):  
P. Fratssinet ◽  
C. Delga ◽  
P. Conte ◽  
N. Rouquet ◽  
M. Nadal
Keyword(s):  
Indirect Immunofluorescence ◽  
Chicken Embryo ◽  
Light Microscope ◽  
Immunofluorescence Staining ◽  
Transparent Material ◽  
Differentiated Cells ◽  
Sem Study ◽  
Indirect Immunofluorescence Staining ◽  
Biocompatibility Testing

Some standards for biocompatibility testing of biomaterials requires the determination of the cytoskeleton aspect of differentiated cells in contact with the material. It is usually determined by indirect immunofluorescence staining of cytoskeleton proteins and examination with a light microscope.This examination is difficult on non transparent material and the resolution is very poor. In order to study the interaction of osteoblast cytoskeleton with HA-macroporous ceramics, we grew chicken embryo osteoblasts on the material then we extracted cell membranes to expose the cytoskeleton proteins. They were then examined in SEM.

Indirect Immunofluorescence Staining of Cultured Neural Cells

2012 ◽  
pp. 235-246 ◽  
Author(s):  
Massimo Barbierato ◽  
Carla Argentini ◽  
Stephen D. Skaper
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Staining ◽  
Neural Cells ◽  
Indirect Immunofluorescence Staining

HLA-B27 typing by use of flow cytofluorometry.

1987 ◽  
Vol 33 (9) ◽  
pp. 1619-1623 ◽  
Author(s):  
J Albrecht ◽  
H A Müller
Keyword(s):  
Indirect Immunofluorescence ◽  
Hla Typing ◽  
Immunofluorescence Staining ◽  
Cytotoxicity Test ◽  
Hla B27 ◽  
Cell Surfaces ◽  
Flow Cytofluorometry ◽  
Indirect Immunofluorescence Staining ◽  
The Cross ◽  
Total Agreement

Abstract We describe the use of flow cytofluorometry to type lymphocytes for HLA-B27 antigens. We modified the cytotoxicity test of Terasaki and McClelland [Nature (London) 1964;204:998] for use with a flow cytofluorometer. In a second assay we used a commercially available monoclonal anti-HLA-B27 antibody and indirect immunofluorescence staining of cell surfaces. Although this antibody cross reacts with B7 and Bw22 antigens, the results for HLA-B27 and the cross-reacting antigens can be separated. Comparison of our results with those by a HLA-typing laboratory for 100 patients showed total agreement of the assigned typings.

Preliminary investigation of the use of indirect immunofluorescence to detect conidia of Brunchorstiapinea

1989 ◽  
Vol 19 (3) ◽  
pp. 389-392 ◽  
Author(s):  
Carol L. Nelson ◽  
John D. Castello ◽  
Paul D. Manion
Keyword(s):  
New York ◽  
Indirect Immunofluorescence ◽  
Preliminary Investigation ◽  
New York State ◽  
Protein A ◽  
Fluorescein Isothiocyanate ◽  
Cross Reactivity ◽  
Staining Procedure ◽  
Spore Trap ◽  
Indirect Immunofluorescence Staining

An indirect immunofluorescence staining procedure was developed for detection of Brunchorstiapinea conidia, using antiserum to conidia and a commercially prepared fluorescein isothiocyanate protein A conjugate. The procedure detected B. pinea conidia from culture, pycnidia, and spore-trap collections. Although cross reactivity occurred with spores of Fusarium spp., Sirococcus sp., Phialophora sp., Gliocladium sp., Verticillium sp., and Gelatinosporium sp., these spores were easily distinguished from those of B. pinea by size, shape, septation, and degree of fluorescence. Fluorescent B. pinea like conidia were collected in spore traps located within and outside the New York State quarantine region. However, the identity of B. pinea like conidia could not be corroborated by other methods.

Sign in / Sign up

Export Citation Format

Share Document