Determination of the cooperativity parameter ? from viscometric data of poly-L-glutamic acid-Na

1980 ◽  
Vol 2 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Mitsuru Satoh ◽  
Jiro Komiyama ◽  
Toshiro Iijima
Keyword(s):  
Glutamic Acid ◽  
Cooperativity Parameter

Improved Cleanup and Derivatization for Gas Chromatographic Determination of Monosodium Glutamate in Foods

1983 ◽  
Vol 66 (6) ◽  
pp. 1528-1531 ◽  
Author(s):  
Hiroshi Nakanishi
Keyword(s):  
Glutamic Acid ◽  
Acid Derivative ◽  
Monosodium Glutamate ◽  
Chromatographic Determination ◽  
Flame Ionization Detection ◽  
Gas Chromatograph ◽  
Flame Ionization ◽  
Acetone Water ◽  
Chromatographic Procedure

Abstract A gas chromatographic procedure is described for determining monosodium glutamate (MSG) in several types of food. A sample is extracted with acetone- water (1 + 1). Acetone is evaporated and an aliquot of the extract is buffered with 1M NH4OH-1M NH4CI pH 9 solution, and chromatographed directly on a column of QAE Sephadex A-25 that has been pretreated with the same buffer. MSG is eluted with 0.1N HC1, and a portion of the eluate is evaporated to dryness and reacted with dimethylformamide( DMF)-dimethylacetal to form the glutamic acid derivative, which is injected into a gas chromatograph and measured by flame ionization detection. Recoveries of MSG from sample fortified at 5-500 mg ranged from 92.8 to 100%.

scholarly journals THE DETERMINATION OF d(-)- AND l(+)-GLUTAMIC ACID IN PROTEIN HYDROLYSATES

1947 ◽  
Vol 168 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Max S. Dunn ◽  
Merrill N. Camien ◽  
Camien S. Shankman ◽  
Harriette Block
Keyword(s):  
Glutamic Acid ◽  
Protein Hydrolysates

A highly sensitive glutamic acid biosensor based on the determination of NADH enzymically generated by l-glutamic dehydrogenase

RSC Advances ◽  
2016 ◽  
Vol 6 (51) ◽  
pp. 45829-45834 ◽  
Author(s):  
Xia Lin ◽  
Qinghong Wang ◽  
Shu Zhu ◽  
Juanjuan Xu ◽  
Qiao Xia ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Highly Sensitive ◽  
Glutamic Dehydrogenase

In this article, a sensitive and stereo-selective biosensor for l-glutamic acid (l-Glu) based on the electrochemiluminescence (ECL) of Ru(bpy)32+ has been designed by applying l-glutamic dehydrogenase (GLDH) for enzymatic generation of NADH in situ.

scholarly journals Evaluation of Two Nonisotopic Immunoassays for Determination of Glutamic Acid Decarboxylase and Tyrosine Phosphatase Autoantibodies in Serum

2004 ◽  
Vol 50 (8) ◽  
pp. 1378-1382 ◽  
Author(s):  
Xavier Palomer ◽  
Dídac Mauricio ◽  
José Rodríguez-Espinosa ◽  
Edgar Zapico ◽  
Carme Mayoral ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Clinical Laboratory ◽  
Autoimmune Diabetes ◽  
Tyrosine Phosphatase ◽  
Acid Decarboxylase ◽  
Time Resolved ◽  
Radiobinding Assay

Abstract Background: Autoantibodies for the 65-kDa form of glutamic acid decarboxylase (GAD65) and protein tyrosine phosphatase-like protein (IA-2) are measured for risk prediction and diagnosis of autoimmune diabetes mellitus. There is a lack of adequate nonisotopic alternatives to the most widely used method for both autoantibodies, which is a radiobinding assay (RBA). Methods: We compared two commercially available immunoassays, an ELISA and a time-resolved immunofluorometric assay (TR-IFMA), with RBA. Results: We found excellent agreement between the RBA and ELISA for measurement of GAD65 autoantibodies (GADAs); they showed comparable analytical precision in the cutoff range and achieved similar diagnostic specificity. The ELISA identified more GADA-positive individuals among patients with new-onset type 1 diabetes than did the RBA [89% (95% confidence interval, 78–95%), vs 71% (58–82%); P <0.03]. For IA-2 autoantibodies (IA-2As), only the TR-IFMA achieved analytical performance and diagnostic accuracy comparable to that of the RBA. These results with the GADA ELISA and IA-2A TR-IFMA were consistent with those obtained blindly in the Diabetes Antibody Standardization Program 2003. The performance of the GADA TR-IFMA and IA-2A ELISA was unsatisfactory, and these tests were not subjected to clinical evaluation. Conclusions: The GADA ELISA and IA-2A TR-IFMA behave comparably with RBA and are thus suitable for use in the clinical laboratory.

Precipitation of αl-glutamic acid: determination of growth kinetics

2007 ◽  
Vol 136 ◽  
pp. 247 ◽  
Author(s):  
Jochen Schöll ◽  
Christian Lindenberg ◽  
Lars Vicum ◽  
Jörg Brozio ◽  
Marco Mazzotti
Keyword(s):  
Glutamic Acid ◽  
Growth Kinetics ◽  
Acid Determination

The Oocytes of the Goby Pomatoschistus minutus. III. Determination of Amino Acid Component

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1094-1095
Author(s):  
Rüdiger Riehl
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Acid Component ◽  
Pomatoschistus Minutus ◽  
Protein Amino Acids ◽  
Amino Acid Component

Abstract The oocytes of the marine goby Pomatoschistus minutus were analyzed for their amino acid content. Most of the amino acids exist as protein, only a little part is free or peptide-bound. Among the protein-bound amino acids, high levels of glutamic acid, proline, alanine, aspartic acid, valine and leucine were detected. These represent more than 60% of the protein amino acids. Among the free acids, glutamic acid, serine and alanine, are dominant. There are no certain proofs of the occurrence of peptide pools in the oocytes of Pomatoschistus minutus.

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