High level extracellular secretion of thermostable ?-amylase ofBacillus stearothermophilus inEscherichia coli

1989 ◽  
Vol 11 (6) ◽  
pp. 383-388 ◽  
Author(s):  
Sribir Sen ◽  
Patrick Oriel
Keyword(s):  
Inescherichia Coli ◽  
Extracellular Secretion ◽  
Ofbacillus Stearothermophilus ◽  
High Level ◽  
Thermostable Amylase

scholarly journals MTMS-Based Aerogel Constructs for Immobilization of Plant Hairy Roots: Effects on Proliferation of Rindera graeca Biomass and Extracellular Secretion of Naphthoquinones

2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Bartosz Nowak ◽  
Mateusz Kawka ◽  
Kamil Wierzchowski ◽  
Katarzyna Sykłowska-Baranek ◽  
Maciej Pilarek
Keyword(s):  
Polyurethane Foam ◽  
Bioactive Compounds ◽  
Hairy Roots ◽  
Reference System ◽  
Culture System ◽  
In Vitro Cultures ◽  
Fresh Mass ◽  
Extracellular Secretion ◽  
High Level

Unique biosynthetic abilities revealed by plants determine in vitro cultures of hairy roots as a suitable source of pharmaceutically relevant bioactive compounds. The basic aim of the study was to examine the applicability of aerogel composed of methyltrimethoxysilane (MTMS) for immobilization of Rindera graeca hairy roots by identifying quantitative effects of biomass proliferation and naphthoquinones extracellular secretion in the aerogel-supported culture system. R. graeca hairy roots were simultaneously cultured for 28-days, as (i) nonimmobilized biomass (reference system), (ii) biomass immobilized on macroporous polyurethane foam (PUF), (iii) biomass with disintegrated MTMS aerogel, (iv) biomass immobilized on polypropylene (PP) fibers (as control), and (v) biomass immobilized on monolithic PP-reinforced MTMS aerogel. MTMS aerogel exhibited high level of biocompatibility toward R. graeca hairy roots which grew into the structure of monolithic aerogel-based constructs. Monolithic MTMS-based constructs significantly promoted the proliferation of hairy roots, resulting in 55% higher fresh mass than the reference system. The highest level of naphthoquinones productivity, i.e., 653 µg gDW−1, was noted for PUF-supported culture system.

High-level extracellular secretion of organophosphorous hydrolase ofFlavobacteriumsp. inEscherichia coliBL21(DE3)pLysS

2016 ◽  
Vol 63 (6) ◽  
pp. 870-876 ◽  
Author(s):  
Soudeh Khanamani Falahati-Pour ◽  
Abbas Sahebghadam Lotfi ◽  
Gholamreza Ahmadian ◽  
Amin Baghizadeh ◽  
Reza Behroozi ◽  
...  
Keyword(s):  
Extracellular Secretion ◽  
High Level

scholarly journals Amino acid misincorporation during high-level expression of mouse epidermal growth factor inEscherichia coli

1991 ◽  
Vol 19 (13) ◽  
pp. 3511-3516 ◽  
Author(s):  
Carol A. Scorer ◽  
Martin J. Carrier ◽  
Robert F. Rosenberger
Keyword(s):  
Amino Acid ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Inescherichia Coli ◽  
High Level Expression ◽  
Epidermal Growth ◽  
High Level

Very high-level production and export inEscherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system

1997 ◽  
Vol 55 (6) ◽  
pp. 854-863 ◽  
Author(s):  
Diane Hasenwinkle ◽  
Eric Jervis ◽  
Oliver Kops ◽  
Chang Liu ◽  
Gary Lesnicki ◽  
...  
Keyword(s):  
Binding Domain ◽  
Fusion System ◽  
Inescherichia Coli ◽  
Cellulose Binding Domain ◽  
High Level ◽  
Cellulose Binding ◽  
Level Production ◽  
Very High

scholarly journals High Level Expression and Refolding of Mouse Interleukin 4 Synthesized inEscherichia coli

1995 ◽  
Vol 270 (13) ◽  
pp. 7445-7452 ◽  
Author(s):  
Alan D. Levine ◽  
Shaukat H. Rangwala ◽  
Nancy A. Horn ◽  
Michelle A. Peel ◽  
Brian K. Matthews ◽  
...  
Keyword(s):  
Interleukin 4 ◽  
Inescherichia Coli ◽  
High Level Expression ◽  
High Level

Construction of a Plasmid for High Level Expression of XMP Aminase inEscherichia coli

1986 ◽  
Vol 50 (7) ◽  
pp. 1879-1884
Author(s):  
Akihiko Maruyama ◽  
Tatsunari Nishi ◽  
Akio Ozaki ◽  
Seiga Ito ◽  
Tatsuro Fujio
Keyword(s):  
Inescherichia Coli ◽  
High Level Expression ◽  
High Level

Gene Synthesis by a LCR-Based Approach: High-Level Production of Leptin-L54 Using Synthetic Gene inEscherichia coli

1998 ◽  
Vol 248 (1) ◽  
pp. 200-203 ◽  
Author(s):  
Lo-Chun Au ◽  
Feng-Yuan Yang ◽  
Wan-Jung Yang ◽  
Sue-Hwa Lo ◽  
Charng-Feng Kao
Keyword(s):  
Synthetic Gene ◽  
Gene Synthesis ◽  
Inescherichia Coli ◽  
High Level ◽  
Level Production

High-Level Expression and Efficient Purification of Recombinant Somatostatin inEscherichia Coli

1989 ◽  
Vol 5 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Joji Notani ◽  
Yoshimasa Saitoh ◽  
Masafumi Terada ◽  
Hisashi Yamada ◽  
Yoshinari Ishii ◽  
...  
Keyword(s):  
Inescherichia Coli ◽  
High Level Expression ◽  
High Level
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