scholarly journals MTMS-Based Aerogel Constructs for Immobilization of Plant Hairy Roots: Effects on Proliferation of Rindera graeca Biomass and Extracellular Secretion of Naphthoquinones

2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Bartosz Nowak ◽  
Mateusz Kawka ◽  
Kamil Wierzchowski ◽  
Katarzyna Sykłowska-Baranek ◽  
Maciej Pilarek
Keyword(s):  
Polyurethane Foam ◽  
Bioactive Compounds ◽  
Hairy Roots ◽  
Reference System ◽  
Culture System ◽  
In Vitro Cultures ◽  
Fresh Mass ◽  
Extracellular Secretion ◽  
High Level

Unique biosynthetic abilities revealed by plants determine in vitro cultures of hairy roots as a suitable source of pharmaceutically relevant bioactive compounds. The basic aim of the study was to examine the applicability of aerogel composed of methyltrimethoxysilane (MTMS) for immobilization of Rindera graeca hairy roots by identifying quantitative effects of biomass proliferation and naphthoquinones extracellular secretion in the aerogel-supported culture system. R. graeca hairy roots were simultaneously cultured for 28-days, as (i) nonimmobilized biomass (reference system), (ii) biomass immobilized on macroporous polyurethane foam (PUF), (iii) biomass with disintegrated MTMS aerogel, (iv) biomass immobilized on polypropylene (PP) fibers (as control), and (v) biomass immobilized on monolithic PP-reinforced MTMS aerogel. MTMS aerogel exhibited high level of biocompatibility toward R. graeca hairy roots which grew into the structure of monolithic aerogel-based constructs. Monolithic MTMS-based constructs significantly promoted the proliferation of hairy roots, resulting in 55% higher fresh mass than the reference system. The highest level of naphthoquinones productivity, i.e., 653 µg gDW−1, was noted for PUF-supported culture system.

An In Vitro Culture System for the Conservation and Production of Bioactive Compounds from Lilium dauricum

2019 ◽  
Vol 27 (4) ◽  
pp. 285-295
Author(s):  
Jin-Ho Kim ◽  
◽  
Thanh-Tam Ho ◽  
Eun Bi Jang ◽  
Seolah Kim ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Culture System

scholarly journals Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jing Yang ◽  
Xiaozeng Yang ◽  
Bin Li ◽  
Xiayang Lu ◽  
Jiefang Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hairy Roots ◽  
Culture System ◽  
Codonopsis Pilosula ◽  
In Vitro Culture System ◽  
Transgenic Hairy Roots

The effect of pre-ovulatory nutrition on the subsequent development of superovulated sheep ova in an in vitro culture system.

1994 ◽  
Vol 1994 ◽  
pp. 82-82
Author(s):  
J. Creed ◽  
T.G. McEvoy ◽  
J.J. Robinson ◽  
R.P. Aitken ◽  
R.M. Palmer ◽  
...  
Keyword(s):  
Food Intake ◽  
Oocyte Maturation ◽  
Culture System ◽  
Subsequent Development ◽  
Plasma Progesterone ◽  
Wide Range ◽  
In Vitro Culture System ◽  
High Level ◽  
The Relationship

Superovulatory treatments for ewes are normally preceded by a period of priming. In a recent study involving two contrasting levels of feeding (0.6 versus 2.4 x maintenance), McEvoy et al (1993) observed that the higher level of feeding suppressed pre-ovulatory plasma progesterone concentrations and the subsequent early development and viability of fertilized ova. This finding suggests that there is a need to reconsider the recommendation, based on data for spontaneously-ovulating ewes, that ‘superovulated embryo donor ewes’ should be maintained on a high level of feeding during the period of oocyte maturation. It also raises questions regarding the form of the relationship between food intake and plasma progesterone concentrations over the wide range of feeding levels that occur in practice. The aims of the present study were therefore two-fold; firstly, to investigate the relationship between level of feeding and plasma progesterone for feed intakes that ranged from 0.6 x maintenance (M) to 2.4 M and secondly to assess the effect of pre-ovulatory feeding levels on the number, quality and viability of ova produced following superovulation.

scholarly journals EFEKTIVITAS MERKURI KLORIDA (HgCl2) PADA STERILISASI TUNAS SAMPING JATI (Tectona grandis) IN VITRO

2017 ◽  
Vol 4 (2) ◽  
pp. 25
Author(s):  
Yusuf Sigit Ahmad Fauzan ◽  
. Supriyanto ◽  
Teuku Tajuddin
Keyword(s):  
In Vitro Culture ◽  
Tectona Grandis ◽  
In Vitro Cultures ◽  
Mercury Chloride ◽  
Main Obstacle ◽  
Sterilization Process ◽  
High Level ◽  
Growth Of Fungi ◽  
Tectona Grandis L.F

Effectiveness of Mercury Chloride (HgCl2) in Sterilization of Teak (Tectona grandis L.f.) In VitroThe main obstacle in obtaining sterile materials in in vitro cultures derived from meristems is high level of surface contamination caused by fungi and bacteria, which often results in explant death. The objective of this study was to obtain an appropriate mercury chloride (HgCl2) concentration for the sterilization of Tectona grandis nodes in in vitro culture. One cm long-sized nodes with 0.2 mm diameter were immersed in HgCl2at concentrations of 0, 100, 200 and 300 mg/L for 3 minutes. The results showed that the higher concentration of HgCl2was able to suppress the growth of fungi and bacteria and increased the percentage of aseptic explants. The best HgCl2concentration was 300 mg/L since it suppressed the growth of fungi and bacteria up to 100% and 75%, respectively, and produced the highest aseptic explants of 85% at 9 days after treatment. The small sized explants supported the sterilization process and reduced browning levels.Keywords: Browning, HgCl2, in vitro, sterilization, T. grandisABSTRAKKendala utama dalam mendapatkan material steril pada kultur in vitro yang berasal dari meristem adalah tingginya tingkat kontaminasi permukaan yang disebabkan oleh jamur dan bakteri, dan sering menyebabkan kematian eksplan. Tujuan penelitian ini adalah untuk memperoleh konsentrasi merkuri klorida (HgCl2) yang tepat untuk sterilisasi eksplan tunas samping tanaman jati (Tectona grandis) pada kultur in vitro. Tunas samping berukuran 1 cm dan diameter 0,2 mm direndam dalam HgCl2 pada konsentrasi 0, 100, 200 dan 300 mg/L selama 3 menit. Hasil penelitian menunjukkan bahwa penambahan konsentrasi HgCl2 yang semakin tinggi mampu menekan pertumbuhan jamur dan bakteri pada eksplan serta meningkatkan persentase eksplan aseptik. HgCl2 dengan konsentrasi 300 mg/L merupakan konsentrasi terbaik karena dapat menekan pertumbuhan jamur hingga 100% dan bakteri mencapai 75%, serta menghasilkan tingkat eksplan aseptik dan hidup tertinggi yaitu sebesar 85% pada 9 hari setelah perlakuan. Ukuran eksplan yang kecil mendukung proses sterilisasi dan mengurangi tingkat browning. Kata kunci: HgCl2,in vitro, pencoklatan jaringan, sterilisasi, T. grandis, Received: 02 November 2017                 Accepted: 14 December 2017                Published: 29 December 2017

scholarly journals Transgenic shoots and plants as a source of natural phytochemical products

2014 ◽  
Vol 69 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Katarzyna Floryanowicz-Czekalska ◽  
Halina Wysokińska
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Secondary Metabolites ◽  
Genetic Engineering ◽  
Bioactive Compounds ◽  
In Vitro Cultures ◽  
Background Information ◽  
Transformation Techniques ◽  
Aerial Parts ◽  
Whole Plants

Genetic engineering has allowed the production of plants and in vitro cultures with an altered content of secondary metabolites. In the present work it is hoped to give some detailed background information on obtaining bioactive compounds based on the use of genetically transformed shoots and the whole plants. <em>Agrobacterium tumefaciens</em>-mediated shoots have recently been a matter of great interest as a source of chemicals synthesized in the aerial parts of plants. The possibilities for the future exploitation of <em>Agrobacterium tumefaciens</em> transformation techniques are enormous. However, we need more knowledge of genes and enzymes controlling secondary metabolic synthesis.

Mechanical Stimulation Enhances the Production of BMP-2 in Ossifying Rat Bone Marrow Organ Cultures

2009 ◽  
Author(s):  
Umut A. Gurkan ◽  
Adam Krueger ◽  
Ozan Akkus
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Culture System ◽  
In Vitro Cultures ◽  
Organ Cultures ◽  
Organ Culture System ◽  
Osteogenic Factors ◽  
Rat Bone

Bone marrow is a reservoir of mesenchymal stem cells and osteoprogenitors. It was previously shown [1, 2] and we have recently verified that in vitro cultures of bone marrow undergo osteogenesis without addition of osteoinductive stimulants. We hypothesized that the in vitro ossifying bone marrow organ culture system can be used as a model to investigate the dynamics of the osteogenesis process and its mechanoresponsiveness in terms of expression of key osteoinductive factors. The outcomes of these studies can be used to develop more effective tissue engineered substitutes for bone regeneration by the utilization of multiple osteogenic factors with complex/sequential delivery mechanisms.

scholarly journals The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

2021 ◽  
Vol 28 (2) ◽  
pp. 110
Author(s):  
Anna Widyastuti ◽  
Afiatry Putrika ◽  
Astari Dwiranti ◽  
Andi Salamah ◽  
Niarsi Merry Hemelda ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Shoot Formation ◽  
Common Type ◽  
In Vitro Cultures ◽  
Sterilization Process ◽  
Bacteria And Fungi ◽  
High Level ◽  
Viable Culture

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.

scholarly journals Soybean Callus—A Potential Source of Tocopherols

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2571
Author(s):  
Liliana Mureșan ◽  
Doina Clapa ◽  
Teodor Rusu ◽  
Thomas T. Y. Wang ◽  
Jae B. Park
Keyword(s):  
Effective Means ◽  
Conventional Tillage ◽  
In Vitro Cultures ◽  
Soybean Seeds ◽  
Soybean Callus ◽  
Potential Source ◽  
Callus Production ◽  
Tillage System ◽  
High Level

In vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were compared to those of soybeans cultivated using the conventional tillage system with three weed controls (respectively without herbicide and with two variants of herbicide). Soybean callus was produced using Murashige and Skoog 1962 (MS) medium supplemented with 0.1 mg/L 6-Benzylaminopurine (BAP) and 0. 1 mg/L Thidiazuron (TDZ). The highest amount of fresh callus was obtained from soybeans from the conventional tillage system with second weed control (S-metolachlor 960 g/L, imazamox 40 g/L, and propaquizafop 100 g/L) respectively 13,652.4 ± 1177.62 mg. The analyzed tocopherols were in much higher content in soy dry callus than the soybean seeds (5.63 µg/g compared with the 0.35 α-toco in soybean, 47.57 µg/g compared with 18.71 µg/g γ-toco or, 5.56 µg/g compared with 1.74 µg/g β-toco). The highest content of the three analyzed tocopherols was γ -tocopherol, both in callus and soybeans. Furthermore, the data showed that herbicides used in soybean culture significantly influenced both the in vitro callus production and the tocopherol callus content (p ˂ 0.05). Altogether, soybean callus can be an important source of tocopherols, and herbicides significantly influence in vitro callus production and the tocopherol callus content.

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