A histochemical study of the nucleic acid content in monkeys with acute radiation sickness

1961 ◽  
Vol 51 (1) ◽  
pp. 29-33
Author(s):  
N. N. Kurshakova
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Histochemical Study ◽  
Acute Radiation ◽  
Radiation Sickness ◽  
Nucleic Acid Content ◽  
Acute Radiation Sickness

Application of Ogur & Rosen's Method for the Determination of Nucleic Acid Content of Bacteria

1957 ◽  
Vol 12 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Nobuyasu KAWASAKI ◽  
Ichiro TAKI ◽  
Chiaki WATANABE ◽  
Kiyoshi MATOBA ◽  
Mokichiro NISHIO ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content

scholarly journals Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

2018 ◽  
Author(s):  
Arnaldo Negron ◽  
Natasha DeLeon-Rodriguez ◽  
Samantha M. Waters ◽  
Luke D. Ziemba ◽  
Bruce Anderson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Acid Content ◽  
Fungal Spores ◽  
Epifluorescence Microscopy ◽  
Nucleic Acid Content ◽  
Rain Event ◽  
Bacterial Cells ◽  
Sampling System ◽  
Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

Ratiometric Fluorescence Imaging for the Distribution of Nucleic Acid Content in Living Cells and Human Tissue Sections

2020 ◽  
Author(s):  
Liu-Yi Liu ◽  
Yuping Zhao ◽  
Nan Zhang ◽  
Kang-Nan Wang ◽  
Minggang Tian ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Imaging ◽  
Human Tissue ◽  
Acid Content ◽  
Living Cells ◽  
Nucleic Acid Content ◽  
Ratiometric Fluorescence ◽  
Tissue Sections

Analysis of the molecular basis of Saccharomyces cerevisiae mutant with high nucleic acid content by comparative transcriptomics

2021 ◽  
Vol 142 ◽  
pp. 110188
Author(s):  
Xuewu Guo ◽  
Bin Zhao ◽  
Xinran Zhou ◽  
Dongxia Lu ◽  
Yaping Wang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleic Acid ◽  
Acid Content ◽  
Molecular Basis ◽  
Comparative Transcriptomics ◽  
Nucleic Acid Content

Age-Related Effect of Dietary Nucleotides on Liver Nucleic Acid Content in Rats

1997 ◽  
Vol 41 (5) ◽  
pp. 324-330 ◽  
Author(s):  
Ana T. López-Navarro ◽  
Angel Gil ◽  
Antonio Sánchez-Pozo
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Related Effect ◽  
Age Related ◽  
Dietary Nucleotides

Change in cholinesterase activity in acute radiation sickness

1959 ◽  
Vol 48 (3) ◽  
pp. 1077-1080
Author(s):  
A. S. Mozzhukin ◽  
D. L. Pevzner
Keyword(s):  
Cholinesterase Activity ◽  
Acute Radiation ◽  
Radiation Sickness ◽  
Acute Radiation Sickness

scholarly journals The protein composition and nucleic acid content of the rat uterus in different states

1957 ◽  
Vol 65 (3) ◽  
pp. 540-545 ◽  
Author(s):  
D. M. Needham ◽  
J. M. Cawkwell
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Protein Composition ◽  
Nucleic Acid Content ◽  
Rat Uterus

scholarly journals Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats

1983 ◽  
Vol 212 (1) ◽  
pp. 149-153 ◽  
Author(s):  
M E Brosnan ◽  
R Farrell ◽  
H Wilansky ◽  
D H Williamson
Keyword(s):  
Mammary Gland ◽  
Nucleic Acid ◽  
Ornithine Decarboxylase ◽  
Acid Content ◽  
Marked Decrease ◽  
Mammary Glands ◽  
Nucleic Acid Content ◽  
Decarboxylase Activity ◽  
Ornithine Decarboxylase Activity ◽  
Polyamine Content

Starvation caused a marked increase in putrescine content in mammary gland of lactating rats, together with a marked decrease in activity of ornithine decarboxylase and appearance of measurable ornithine decarboxylase antizyme. 2. Refeeding for 5 h caused disappearance of free antizyme and ornithine decarboxylase activity returned to the value in fed animals. Putrescine concentration remained elevated. 3. There was no significant change in nucleic acid content of mammary gland from starved rats, but spermidine and spermine contents increased significantly. 4. Refeeding for 5 h returned the spermidine content of mammary glands to ‘fed’ values, and significantly decreased the content of spermine, although it did not reach control values. Thus changes in polyamine content of mammary gland in starved rats are clearly dissociated from changes in either RNA content or activities of polyamine-synthetic decarboxylases. 5. Starvation caused a fall in the content of spermidine in liver, with no change in spermine content. Refeeding for 5 h returned the spermidine content to ‘fed’ values.

Effect of Choline Deficiency and Ethionine Feeding on Nucleic Acid Content of Rat Livers

1961 ◽  
Vol 73 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Preston T. Farish ◽  
W. D. Salmon ◽  
H. E. Sauberlich
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Choline Deficiency ◽  
Rat Livers
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