Characterization of three different transgenic mouse lines that carry human poliovirus receptor gene?influence of the transgene expression on pathogenesis

1994 ◽  
Vol 139 (3-4) ◽  
pp. 351-363 ◽  
Author(s):  
S. Koike ◽  
C. Taya ◽  
J. Aoki ◽  
Y. Matsuda ◽  
I. Ise ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Transgene Expression ◽  
Receptor Gene ◽  
Poliovirus Receptor ◽  
Mouse Lines

Characterization of mouse lines transgenic with the human poliovirus receptor gene

1998 ◽  
Vol 25 (1) ◽  
pp. 43-54 ◽  
Author(s):  
A.M Deatly ◽  
R.E Taffs ◽  
J.M McAuliffe ◽  
S.P Nawoschik ◽  
J.W Coleman ◽  
...  
Keyword(s):  
Receptor Gene ◽  
Poliovirus Receptor ◽  
Mouse Lines

scholarly journals Characterization of Learning, Motivation, and Visual Perception in Five Transgenic Mouse Lines Expressing GCaMP in Distinct Cell Populations

2020 ◽  
Vol 14 ◽  
Author(s):  
Peter A. Groblewski ◽  
Douglas R. Ollerenshaw ◽  
Justin T. Kiggins ◽  
Marina E. Garrett ◽  
Chris Mochizuki ◽  
...  
Keyword(s):  
Visual Perception ◽  
Transgenic Mouse ◽  
Learning Motivation ◽  
Cell Populations ◽  
Distinct Cell ◽  
Mouse Lines

Characterization of different transgenic mouse lines, overexpressing the FAD-linked human presenilin 1 CDNA

2000 ◽  
Vol 21 ◽  
pp. 226
Author(s):  
Christine Sturchler-Pierrat ◽  
Dorothee Abramowski ◽  
Christophe Wiessner ◽  
Matthias Staufenbiel ◽  
Karl-Heinz Wiederhold ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Presenilin 1 ◽  
Mouse Lines

scholarly journals Ectopic transgene expression in the retina of four transgenic mouse lines

2015 ◽  
Vol 221 (7) ◽  
pp. 3729-3741 ◽  
Author(s):  
Robert Gábriel ◽  
Ferenc Erdélyi ◽  
Gábor Szabó ◽  
J. Josh Lawrence ◽  
Márta Wilhelm
Keyword(s):  
Transgenic Mouse ◽  
Transgene Expression ◽  
Mouse Lines

scholarly journals Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors

2019 ◽  
Vol 116 (52) ◽  
pp. 27001-27010 ◽  
Author(s):  
Kyle B. Fischer ◽  
Hannah K. Collins ◽  
Edward M. Callaway
Keyword(s):  
Gene Expression ◽  
Transgenic Mouse ◽  
Transgene Expression ◽  
Cell Types ◽  
Cell Type ◽  
Recognition Sites ◽  
Highly Sensitive ◽  
Plasmid Production ◽  
Targeted Gene Expression ◽  
Mouse Lines

In combination with transgenic mouse lines expressing Cre or Flp recombinases in defined cell types, recombinase-dependent adeno-associated viruses (AAVs) have become the tool of choice for localized cell-type-targeted gene expression. Unfortunately, applications of this technique when expressing highly sensitive transgenes are impeded by off-target, or “leak” expression, from recombinase-dependent AAVs. We investigated this phenomenon and find that leak expression is mediated by both infrequent transcription from the inverted transgene in recombinant-dependent AAV designs and recombination events during bacterial AAV plasmid production. Recombination in bacteria is mediated by homology across the antiparallel recombinase-specific recognition sites present in recombinase-dependent designs. To address both of these issues we designed an AAV vector that uses mutant “cross-over insensitive” recognition sites combined with an “ATG-out” design. We show that these CIAO (cross-over insensitive ATG-out) vectors virtually eliminate leak expression. CIAO vectors provide reliable and targeted transgene expression and are extremely useful for recombinase-dependent expression of highly sensitive transgenes.

Establishment and characterization of immortal hepatocytes derived from various transgenic mouse lines

2002 ◽  
Vol 294 (4) ◽  
pp. 864-871 ◽  
Author(s):  
Rainer Klocke ◽  
M José Gómez-Lechón ◽  
Anja Ehrhardt ◽  
Tomas Mendoza-Figueroa ◽  
M Teresa Donato ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Lines

scholarly journals Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pankhuri Vyas ◽  
Jingjing Sherry Wu ◽  
Adrian Jimenez ◽  
Elisabeth Glowatzki ◽  
Paul Albert Fuchs
Keyword(s):  
Transgenic Mouse ◽  
Type I ◽  
Type Ii ◽  
Afferent Neurons ◽  
Mouse Lines
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