Two-dimensional electrophoresis of peptides from human-CHO cell hybrids containing human chromosome 21

1983 ◽  
Vol 9 (6) ◽  
pp. 687-697 ◽  
Author(s):  
C. H. Scoggin ◽  
S. Paul ◽  
Y. E. Miller ◽  
D. Patterson
Keyword(s):  
Human Chromosome ◽  
Chromosome 21 ◽  
Two Dimensional ◽  
Cho Cell ◽  
Two Dimensional Electrophoresis ◽  
Human Chromosome 21 ◽  
Cell Hybrids ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of human-CHO cell hybrids containing human chromosome 11

1981 ◽  
Vol 7 (4) ◽  
pp. 389-398 ◽  
Author(s):  
C. H. Scoggin ◽  
E. Gabrielson ◽  
J. N. Davidson ◽  
C. Jones ◽  
D. Patterson ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Two Dimensional ◽  
Chromosome 11 ◽  
Cho Cell ◽  
Two Dimensional Electrophoresis ◽  
Cell Hybrids ◽  
Dimensional Electrophoresis

Molecular analysis and breakpoint definition of a set of human chromosome 21 somatic cell hybrids

1995 ◽  
Vol 21 (6) ◽  
pp. 415-428 ◽  
Author(s):  
Sharon L. Graw ◽  
Katheleen Gardiner ◽  
Karen Hall-Johnson ◽  
Iris Hart ◽  
Anthony Joetham ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Molecular Analysis ◽  
Chromosome 21 ◽  
Somatic Cell Hybrids ◽  
Human Chromosome 21 ◽  
Cell Hybrids ◽  
Definition Of

Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids

1995 ◽  
Vol 21 (5) ◽  
pp. 357-365
Author(s):  
Isik G. Yulug ◽  
Ann M. Killary ◽  
Arbansjit K. Sandhu ◽  
Raghbir S. Athwal ◽  
Margaret Fox ◽  
...  
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Human Chromosome ◽  
Chromosome 21 ◽  
Human Chromosome 21 ◽  
Cell Hybrids

Assignment of the gene for cystathionine ?-synthase to human chromosome 21 in somatic cell hybrids

1984 ◽  
Vol 65 (3) ◽  
pp. 291-294 ◽  
Author(s):  
Flemming Skovby ◽  
Natalie Krassikoff ◽  
Uta Francke
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Chromosome 21 ◽  
Somatic Cell Hybrids ◽  
Human Chromosome 21 ◽  
Cell Hybrids

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

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