Preparation of tritiated thymidine with high labelling yield via tritiated water

1995 ◽  
Vol 196 (1) ◽  
pp. 113-123
Author(s):  
Y. F. Shafiq
Keyword(s):  
Tritiated Thymidine ◽  
Tritiated Water ◽  
Labelling Yield

Cytological Damage Produced in the Mouse Testes by Tritiated Thymidine, Tritiated Water and X-rays

1969 ◽  
Vol 17 (4) ◽  
pp. 547-557 ◽  
Author(s):  
B. E. Lambert
Keyword(s):  
Tritiated Thymidine ◽  
Tritiated Water ◽  
X Rays

Disappearance of Tritiated Thymidine and Tritiated Water from the Anterior Chamber of the Rabbit Eye

Nature ◽  
1962 ◽  
Vol 196 (4856) ◽  
pp. 786-787 ◽  
Author(s):  
RONALD M. MAENZA ◽  
CLIFFORD V. HARDING
Keyword(s):  
Anterior Chamber ◽  
Tritiated Thymidine ◽  
Tritiated Water ◽  
Rabbit Eye

scholarly journals Tritium retention in femoral bone marrow and spleens of mice receiving single intravenous injections of tritiated water and tritiated thymidine.

1987 ◽  
Vol 28 (3) ◽  
pp. 204-212
Author(s):  
HISAMASA JOSHIMA ◽  
SATORU MATSUSHITA ◽  
KUMIKO FUKUTSU ◽  
MASATOSHI KASHIMA
Keyword(s):  
Bone Marrow ◽  
Tritiated Thymidine ◽  
Tritiated Water ◽  
Femoral Bone ◽  
Intravenous Injections ◽  
Femoral Bone Marrow

Maximum Permissible concentrations for Tritiated Water and Tritiated Thymidine

AIHAJ ◽  
1969 ◽  
Vol 30 (5) ◽  
pp. 514-518
Author(s):  
Donald E. Barber
Keyword(s):  
Tritiated Thymidine ◽  
Tritiated Water ◽  
Maximum Permissible Concentrations

Testis Mass Loss in the Mouse Induced by Tritiated Thymidine, Tritiated Water, and 60Co Gamma Irradiation

1979 ◽  
Vol 36 (2) ◽  
pp. 135-145 ◽  
Author(s):  
T. E. F. Carr ◽  
J. Nolan
Keyword(s):  
Gamma Irradiation ◽  
Mass Loss ◽  
Tritiated Thymidine ◽  
Tritiated Water ◽  
60Co Gamma ◽  
Testis Mass

Comparisons of Tritiated Thymidine, Tritiated Water, and Colbalt-60 Gamma Rays in Inducing Chromosomal Aberrations

1965 ◽  
Vol 24 (2) ◽  
pp. 214 ◽  
Author(s):  
William C. Dewey ◽  
Ronald M. Humphrey ◽  
B. Ann Jones
Keyword(s):  
Gamma Rays ◽  
Chromosomal Aberrations ◽  
Tritiated Thymidine ◽  
Tritiated Water

Spectra of Base Substitution Mutations Induced in Escherichia coli by Tritiated Water and the Decay of Incorporated Tritiated Thymidine

1984 ◽  
Vol 97 (1) ◽  
pp. 200 ◽  
Author(s):  
Tuneo Ise ◽  
Takesi Kato ◽  
Barry W. Glickman
Keyword(s):  
Escherichia Coli ◽  
Tritiated Thymidine ◽  
Tritiated Water ◽  
Base Substitution ◽  
Substitution Mutations

Cell Killing by Gamma Rays and Beta Particles from Tritiated Water and Incorporated Tritiated Thymidine

1975 ◽  
Vol 63 (3) ◽  
pp. 531 ◽  
Author(s):  
Joel S. Bedford ◽  
James B. Mitchell ◽  
H. Gaston Griggs ◽  
Michael A. Bender
Keyword(s):  
Gamma Rays ◽  
Tritiated Thymidine ◽  
Tritiated Water ◽  
Cell Killing ◽  
Beta Particles

Comparative Labelling and Biokinetic Studies of 99mTc-EDTA(Sn) and 99mTc-DTPA(Sn)

1977 ◽  
Vol 16 (01) ◽  
pp. 30-35 ◽  
Author(s):  
N. Agha ◽  
R. B. R. Persson
Keyword(s):  
Complex Formation ◽  
Significant Influence ◽  
Room Temperature ◽  
Ph Value ◽  
Maximum Activity ◽  
Reduction Step ◽  
Labelling Yield ◽  
Different Temperatures ◽  
Kinetics Of

SummaryGelchromatography column scanning has been used to study the fractions of 99mTc-pertechnetate, 99mTcchelate and reduced hydrolyzed 99mTc in preparations of 99mTc-EDTA(Sn) and 99mTc-DTPA(Sn). The labelling yield of 99mTc-EDTA(Sn) chelate was as high as 90—95% when 100 μmol EDTA · H4 and 0.5 (Amol SnCl2 was incubated with 10 ml 99mTceluate for 30—60 min at room temperature. The study of the influence of the pH-value on the fraction of 99mTc-EDTA shows that pH 2.8—2.9 gave the best labelling yield. In a comparative study of the labelling kinetics of 99mTc-EDTA(Sn) and 99mTc- DTPA(Sn) at different temperatures (7, 22 and 37°C), no significant influence on the reduction step was found. The rate constant for complex formation, however, increased more rapidly with increased temperature for 99mTc-DTPA(Sn). At room temperature only a few minutes was required to achieve a high labelling yield with 99mTc-DTPA(Sn) whereas about 60 min was required for 99mTc-EDTA(Sn). Comparative biokinetic studies in rabbits showed that the maximum activity in kidneys is achieved after 12 min with 99mTc-EDTA(Sn) but already after 6 min with 99mTc-DTPA(Sn). The long-term disappearance of 99mTc-DTPA(Sn) from the kidneys is about five times faster than that for 99mTc-EDTA(Sn).

Progestation

1967 ◽  
Vol 56 (3_Suppla) ◽  
pp. S7-S45 ◽  
Keyword(s):  
Tritiated Thymidine ◽  
Succinic Dehydrogenase ◽  
Glandular Epithelium ◽  
Synthetic Activity ◽  
Cell Renewal ◽  
Pulse Labelling ◽  
Uterine Receptivity ◽  
Alkaline Phosphatases ◽  
Monocytic Cells ◽  
Acid And Alkaline Phosphatases

ABSTRACT Autoradiographic, enzymic and histologic studies on uteri of pregnant rats were carried out to follow the endometrial modifications which take place during progestation (days L0 – L4) and culminate in the state of uterine receptivity essential for ovum-implantation. Pulse labelling with tritiated thymidine (radioactive DNA precursor) on L0, L1 and L2 revealed a sequence of cell renewal in luminal and glandular epithelium and endometrial stroma. On L3 and L4 stromal cells showed extensive incorporation of tritiated thymidine. This synthetic activity was associated with endometrial preparation for decidualization and was evoked at least in part, by the surge of oestrogen on L3. All layers of the uterine wall were heavily infiltrated on L0 and resembled the site of an acute inflammatory reaction. Subsequently, polymorphonuclear infiltration diminished and monocytic cells predominated. On L3 a spatial arrangement was observed: eosinophiles were concentrated in the basal endometrium and monocytic cells in the subepithelial stroma. A comparison was made between such a shift in migratory cells in the uterus and similar phenomena which occur in inflammatory and immune reactions. Activities of acid and alkaline phosphatases, of ATP-ase and succinic dehydrogenase were low on L0 and L1 during the periods of infiltration, degeneration and regeneration of luminal and glandular epithelium. Enzymic activities increased on the following days, (L3 and L4). Vascular dilation and engorgement and endometrial oedema were observed near the blastocysts on L4. Most blastocysts incorporated tritiated thymidine after 14.00 h on L4, but some showed uptake before loss of the zona which occurs usually between 14.00 and 16.00 h; therefore, it was assumed that the permeability of the zona increases prior to being shed. Activities of succinic dehydrogenase and acid and alkaline phosphatases were demonstrable in blastocysts on L4 while they were still »free« in the uterine lumen.

Sign in / Sign up

Export Citation Format

Share Document