A single reagent radioimmunoassay for thyroxine in blood samples absorbed on filter paper for mass screening of neonatal hypothyroidism

A screening program for the detection of neonatal hypothyroidism has been in effect in Oregon since May 1975. Blood samples are obtained from all newborn infants to test for phenylketonuria and other metabolic diseases. A second specimen is obtained from more than 90% of these infants who are retested at 4 to 6 weeks of age. These Guthrie filter paper blood samples are analyzed for thyroxine (T4), and all samples with a low T4 value are analyzed for thyroid stimulating hormone (TSH). At the outset of the program, it was speculated that the screening might detect infants who had reduced

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Fluorescence enzyme immunoassay of 17.ALPHA.-hydroxyprogesterone in dried blood samples on filter paper and its application to mass screening for congenital adrenal hyperplasia.

Chemical and Pharmaceutical Bulletin ◽

10.1248/cpb.31.2724 ◽

1983 ◽

Vol 31 (8) ◽

pp. 2724-2731 ◽

Cited By ~ 18

Author(s):

HIDETOSHI ARAKAWA ◽

MASAKO MAEDA ◽

AKIO TSUJI ◽

HIROSHI NARUSE ◽

EMIKO SUZUKI ◽

...

Keyword(s):

Congenital Adrenal Hyperplasia ◽

Enzyme Immunoassay ◽

Mass Screening ◽

Filter Paper ◽

Adrenal Hyperplasia ◽

Blood Samples

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PSA assay of dried blood samples from the ear lobe on a filter paper with special reference to prostatic mass screening

The Prostate ◽

10.1002/pros.2990270205 ◽

1995 ◽

Vol 27 (2) ◽

pp. 90-94 ◽

Cited By ~ 9

Author(s):

Hiroki Watanabe ◽

Hiroshi Ohe ◽

Masahito Saitoh ◽

Munekado Kojima ◽

Toshiyuki Tanaka ◽

...

Keyword(s):

Special Reference ◽

Mass Screening ◽

Filter Paper ◽

Blood Samples

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Enzyme immunoassay of thyroxin-binding globulin in dried blood samples on filter paper.

Clinical Chemistry ◽

10.1093/clinchem/29.7.1437 ◽

1983 ◽

Vol 29 (7) ◽

pp. 1437-1440 ◽

Cited By ~ 3

Author(s):

N Hata ◽

M Ito ◽

H Mizuta ◽

O Nose ◽

K Miyai

Keyword(s):

Enzyme Immunoassay ◽

Filter Paper ◽

Room Temperature ◽

Neonatal Hypothyroidism ◽

Blood Samples ◽

Coefficients Of Variation ◽

The Mean ◽

Positive Results ◽

Measurable Concentration

Abstract A double-antibody enzyme immunoassay was developed for determination of thyroxin-binding globulin in dried blood samples on filter paper. The measurable concentration range of thyroxin-binding globulin in two 3-mm blood discs was 3.3 to 52 mg/L equivalent of serum (i.e., equivalent to the concentrations in known serum standards). Thyroxin-binding globulin in dried blood samples on filter paper was stable for at least four weeks when kept dry at -20 degrees C, 4 degrees C, or room temperature. The mean coefficients of variation were 6.6% (within assay) and 5.9% (between assays). The concentrations of thyroxin-binding globulin in dried blood samples determined by this method correlated well with those in serum determined by radioimmunoassay (r = 0.95) and by enzyme immunoassay (r = 0.96). This method is applicable for detecting cases of thyroxin-binding globulin deficiency and avoids the false-positive results for neonatal hypothyroidism obtained by measuring thyroxin.

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