PSA assay of dried blood samples from the ear lobe on a filter paper with special reference to prostatic mass screening

Abstract We describe an electroimmunodiffusion technique for measuring beta-lipoprotein in cord blood spotted on filter paper. A series of cord-blood samples, taken from 916 consecutive live-birth infants, was spotted directly onto filter paper and assayed for beta-lipoprotein. Eleven had above-normal beta-lipoprotein. Of these 11, seven were tested two to six months later, along with their parents, for total cholesterol and triglyceride concentrations. Five infants had increased cholesterol values, and four parents of these infants had either increased cholesterol or triglyceride values. We also measured beta-lipoprotein concentrations in 63 paired samples of dried cord-blood and three-day post-delivery blood specimens, routinely collected for phenylketonuria screening. We saw a significant correlation between results for the specimens, but detected no cases of increased beta-lipoprotein. beta-Lipoprotein assay in dried specimens of cord blood is simple, inexpensive, and potentially is useful in mass screening of newborns for familial type II and combined hyperlipidemia.

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Determination of amino acids by ion-exchange chromatography on filter paper spotted blood samples stored at different temperatures and for different periods: comparison with capillary and venous blood

Clinical Biochemistry ◽

10.1016/s0009-9120(01)00244-2 ◽

2001 ◽

Vol 34 (5) ◽

pp. 399-406 ◽

Cited By ~ 11

Author(s):

Yousef M Abdulrazzaq ◽

Ahmed Ibrahim

Keyword(s):

Amino Acids ◽

Ion Exchange ◽

Filter Paper ◽

Venous Blood ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Blood Samples ◽

Different Temperatures

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Techniques' Comparison and Report of the North Carolina Experience

PEDIATRICS ◽

10.1542/peds.83.5.843 ◽

1989 ◽

Vol 83 (5) ◽

pp. 843-848

Author(s):

Thomas R. Kinney ◽

Martha Sawtschenko ◽

Mary Whorton ◽

Jean Shearin ◽

Christy Stine ◽

...

Keyword(s):

North Carolina ◽

Filter Paper ◽

Screening Program ◽

Medical Center ◽

Sample Collection ◽

Capillary Tubes ◽

Blood Samples ◽

Screening Programs ◽

The North ◽

Duke University

Controversy still exists as to the best laboratory method to use to screen newborns for sickle cell disease and other hemoglobinopathies. The proposed methods include hemoglobin electrophoresis, column chromatography, isoelectric focusing, and high performance liquid chromatography. There is also debate concerning the preferred method of sample collection. The proposed methods of sample collection include cord blood or blood obtained from the infant collected in a tube with anticoagulant or on filter paper. We compared hemoglobin electrophoresis patterns from infant blood samples collected in heparinized capillary tubes and on filter paper. This comparison was performed because hemoglobin electrophoresis of dried blood samples collected on filter paper has been advocated as a practical, reliable, and inexpensive method for mass screening programs, although the limitations of this technique have not been explored fully. We also summarize data from the North Carolina Newborn Hemoglobinopathy Screening Program, which relates to the advantages and limitations of hemoglobin electrophoresis from filter paper blood specimens. MATERIALS AND METHODS Specimens Four sets of specimens were used for this study: (1) specimens collected at Duke University Medical Center to compare hemoglobin electrophoresis patterns of hemolysates from filter paper and heparinized capillary tubes, (2) specimens collected by the North Carolina program for hemoglobinopathy screening, (3) specimens routinely collected at Duke University in heparinized capillary tubes for newborn hemoglobinopathy screening, and (4) samples for retesting to examine the error rate of the state program and to confirm screening results compatible with a hemoglobinopathy. Samples for Direct Comparison Between Filter Paper and Heparinized Specimens

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