Intracytoplasmic sperm injection improving embryo quality: Comparison of the sibling oocytes of Non-Male-Factor couples

ABSTRACT Aim The aim of this study was to evaluate the influence of spermatozoa morphology on ICSI cycle outcome parameters in couples with male factor infertility. Design Retrospective study. Setting University-level tertiary care center. Patients and methods One hundred and forty-eight couples with male factor infertility who had undergone intracytoplasmic sperm injection (ICSI) cycle from 2010 to December 2012 were included in this analysis. The semen samples of the male partners were classified according to the three predictive categories of the Tygerberg strict criteria: excellent prognosis (> 14% morphologically normal spermatozoa), good prognosis (5–14%) and poor prognosis (< 5%). Main outcome measures The primary outcome was the embryo quality rate. Results Patients in the poor prognosis subgroups exhibited deficits in spermatozoa concentration, motility and total motile fraction. The variations in the outcome parameters of fertilization rate, embryo development rate and embryo quality did not correlate with sperm morphology. Conclusion Our study suggests that Kruger's strict morphology criteria of the fresh semen sample is not a good predictor for the ICSI cycle outcome. How to cite this article Preetha RP, Kamath MS, Aleyamma TK, Muthukumar K. Influence of Sperm Morphology on the Outcome of Assisted Reproductive Technique-Intracytoplasmic Sperm Injection Cycles: A Retrospective Analysis. Int J Infertil Fetal Med 2015;6(3):122-127.

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Intracytoplasmic sperm injection (ICSI) versus high insemination concentration (HIC) for human conception in vitro

Reproduction Fertility and Development ◽

10.1071/rd9950169 ◽

1995 ◽

Vol 7 (2) ◽

pp. 169 ◽

Cited By ~ 13

Author(s):

S Fishel ◽

F Lisi ◽

L Rinaldi ◽

R Lisi ◽

J Timson ◽

...

Keyword(s):

Intracytoplasmic Sperm Injection ◽

Normal Forms ◽

Sperm Morphology ◽

First Line ◽

Viable Option ◽

Male Factor ◽

Cleavage Rate ◽

Vitro Fertilization ◽

Sibling Oocytes

The use of high insemination concentration (HIC) for in vitro fertilization (IVF) was compared with intracytoplasmic sperm injection (ICSI) in cases of male factor infertility. Sibling oocytes (n = 252) from 24 patients were used, 123 for HIC and 129 for ICSI. Although the incidence of fertilization was decreased with HIC (48% v. 61%), this treatment was nevertheless a viable option for many patients, especially when ICSI was not available. However, there was a higher incidence of cytoplasmic fragmentation of embryos after HIC compared with ICSI (36% v. 10%, P = 0.003) and the outcome was significantly affected by the severity of teratozoospermia. Using a cut-off of 5% normal forms, the incidence of fertilization with HIC for the group with < 5% normal forms was 37% compared with 72% for the group with > 5% normal forms; there was also a significant decrease in cleavage rate (P = 0.05) and the number of regular embryos (P = 0.005), and an increase in cytoplasmic fragmentation (P = 0.006) in patients with < 5% normal forms. No distinction was made between cases of teratozoospermia when ICSI was used. The present study confirms the value of HIC as a first line treatment for male infertility, as long as ICSI remains significantly more expensive and concerns on safety are mooted. However, the use of sibling oocytes for ICSI is recommended, especially in cases with < 5% normal sperm morphology.

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In Vitro Fertilisation Versus Intracytoplasmic Sperm Injection in Patients Without Severe Male Factor Infertility

Case Medical Research ◽

10.31525/ct1-nct04128904 ◽

2019 ◽

Author(s):

Keyword(s):

Intracytoplasmic Sperm Injection ◽

In Vitro Fertilisation ◽

Male Factor Infertility ◽

Male Factor

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Comparison of in vitro fertilization/intracytoplasmic sperm injection on live birth rates in couples with non-male factor infertility and advanced maternal age: overlooked details

Journal of Assisted Reproduction and Genetics ◽

10.1007/s10815-021-02210-4 ◽

2021 ◽

Author(s):

Enver Kerem Dirican ◽

Safak Olgan

Keyword(s):

In Vitro Fertilization ◽

Live Birth ◽

Intracytoplasmic Sperm Injection ◽

Maternal Age ◽

Advanced Maternal Age ◽

Male Factor Infertility ◽

Male Factor ◽

Vitro Fertilization ◽

Live Birth Rates

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In vitro fertilisation (IVF) versus intracytoplasmic sperm injection (ICSI) in patients without severe male factor infertility: study protocol for the randomised, controlled, multicentre trial INVICSI

BMJ Open ◽

10.1136/bmjopen-2021-051058 ◽

2021 ◽

Vol 11 (6) ◽

pp. e051058

Author(s):

Sine Berntsen ◽

Bugge Nøhr ◽

Marie Louise Grøndahl ◽

Morten Rønn Petersen ◽

Lars Franch Andersen ◽

...

Keyword(s):

Live Birth ◽

Intracytoplasmic Sperm Injection ◽

Controlled Trial ◽

Male Partner ◽

In Vitro Fertilisation ◽

Male Factor Infertility ◽

Male Factor ◽

Fertilisation Rate ◽

Randomised Controlled

IntroductionOver the last decades, the use of intracytoplasmic sperm injection (ICSI) has increased, even among patients without male factor infertility. The increase has happened even though there is no evidence to support that ICSI results in higher live birth rates compared with conventional in vitro fertilisation (IVF) in cases with nonmale factor infertility. The lack of robust evidence on an advantage of using ICSI over conventional IVF in these patients is problematic since ICSI is more invasive, complex and requires additional resources, time and effort. Therefore, the primary objective of the IVF versus ICSI (INVICSI) study is to determine whether ICSI is superior to standard IVF in patients without severe male factor infertility. The primary outcome measure is first live birth from fresh and frozen-thawed transfers after one stimulated cycle. Secondary outcomes include fertilisation rate, ongoing pregnancy rate, birth weight and congenital anomalies.Methods and analysisThis is a two-armed, multicentre, randomised, controlled trial. In total, 824 couples/women with infertility without severe male factor will be recruited and allocated randomly into two groups (IVF or ICSI) in a 1:1 ratio. Participants will be randomised in variable block sizes and stratified by trial site and age. The main inclusion criteria are (1) no prior IVF/ICSI treatment, (2) male partner sperm with an expected count of minimum 2 million progressive motile spermatozoa following density gradient purification on the day of oocyte pick up and (3) age of the woman between 18 and 42 years.Ethics and disseminationThe study will be performed in accordance with the ethical principles in the Helsinki Declaration. The study is approved by the Scientific Ethical Committee of the Capital Region of Denmark. Study findings will be presented, irrespectively of results at international conferences and submitted for publication in peer-reviewed journals.Trial registration numberNCT04128904. Pre-results.

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Intracytoplasmic sperm injection: state of the art in humans

Reproduction ◽

10.1530/rep-17-0374 ◽

2017 ◽

Vol 154 (6) ◽

pp. F93-F110 ◽

Cited By ~ 28

Author(s):

G D Palermo ◽

C L O’Neill ◽

S Chow ◽

S Cheung ◽

A Parrella ◽

...

Keyword(s):

Intracytoplasmic Sperm Injection ◽

Poor Prognosis ◽

State Of The Art ◽

Semen Analysis ◽

Reproductive Technology ◽

Male Factor Infertility ◽

Male Factor ◽

Current Application ◽

Infertile Couples ◽

The Impact

Among infertile couples, 25% involve both male and female factors, while male factor alone accounts for another 25% due to oligo-, astheno-, teratozoospermia, a combination of the three, or even a complete absence of sperm cells in the ejaculate and can lead to a poor prognosis even with the help of assisted reproductive technology (ART). Intracytoplasmic sperm injection (ICSI) has been with us now for a quarter of a century and in spite of the controversy generated since its inception, it remains in the forefront of the techniques utilized in ART. The development of ICSI in 1992 has drastically decreased the impact of male factor, resulting in millions of pregnancies worldwide for couples who, without ICSI, would have had little chance of having their own biological child. This review focuses on the state of the art of ICSI regarding utility of bioassays that evaluate male factor infertility beyond the standard semen analysis and describes the current application and advances in regard to ICSI, particularly the genetic and epigenetic characteristics of spermatozoa and their impact on reproductive outcome.

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