Sequence analysis and phylogenetic reconstruction of the genes encoding the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase from the chlorophyllb-containing prokaryoteProchlorothrix hollandica

1991 ◽  
Vol 32 (5) ◽  
pp. 379-395 ◽  
Author(s):  
Clifford W. Morden ◽  
Susan S. Golden
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Reconstruction ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding

scholarly journals Genomic organization, sequence analysis and expression of all five genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from tomato

1987 ◽  
Vol 209 (2) ◽  
pp. 247-256 ◽  
Author(s):  
Mamoru Sugita ◽  
Thianda Manzara ◽  
Eran Pichersky ◽  
Anthony Cashmore ◽  
Wilhelm Gruissem
Keyword(s):  
Sequence Analysis ◽  
Genomic Organization ◽  
Small Subunit ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding

scholarly journals Methylotrophic Autotrophy in Beijerinckia mobilis

2005 ◽  
Vol 187 (11) ◽  
pp. 3884-3888 ◽  
Author(s):  
Svetlana N. Dedysh ◽  
Ksenia V. Smirnova ◽  
Valentina N. Khmelenina ◽  
Natalia E. Suzina ◽  
Werner Liesack ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Sequence Analysis ◽  
Methanol Dehydrogenase ◽  
Partial Sequence ◽  
Ribulose Bisphosphate ◽  
Bisphosphate Carboxylase ◽  
Oxidation Of Methanol ◽  
Cell Extracts ◽  
Wide Range ◽  
Genes Encoding

ABSTRACT Representatives of the genus Beijerinckia are known as heterotrophic, dinitrogen-fixing bacteria which utilize a wide range of multicarbon compounds. Here we show that at least one of the currently known species of this genus, i.e., Beijerinckia mobilis, is also capable of methylotrophic metabolism coupled with the ribulose bisphosphate (RuBP) pathway of C1 assimilation. A complete suite of dehydrogenases commonly involved in the sequential oxidation of methanol via formaldehyde and formate to CO2 was detected in cell extracts of B. mobilis grown on CH3OH. Carbon dioxide produced by oxidation of methanol was further assimilated via the RuBP pathway as evidenced by reasonably high activities of phosphoribulokinase and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). Detection and partial sequence analysis of genes encoding the large subunits of methanol dehydrogenase (mxaF) and form I RubisCO (cbbL) provided genotypic evidence for methylotrophic autotrophy in B. mobilis.

scholarly journals Variation of gonococcal lipooligosaccharide structure is due to alterations in poly-G tracts in lgt genes encoding glycosyl transferases.

1996 ◽  
Vol 183 (1) ◽  
pp. 323-327 ◽  
Author(s):  
Q L Yang ◽  
E C Gotschlich
Keyword(s):  
Sequence Analysis ◽  
Neisseria Gonorrhoeae ◽  
Experimental Evidence ◽  
High Frequency ◽  
Genetic Mechanism ◽  
Frequency Variation ◽  
Alpha Chain ◽  
Glycosyl Transferases ◽  
Genes Encoding

The lipooligosaccharide (LOS) expressed by gonococci spontaneously varies its structure at high frequency, but the underlying genetic mechanism has not been described. We have previously reported that the genes encoding the glycosyl transferases responsible for the biosynthesis of the variable alpha chain of the LOS of Neisseria gonorrhoeae are located in a locus containing five genes, lgtA, lgtB, lgtC, lgtD, and lgtE. Sequence analysis showed that lgtA, lgtC, and lgtD contained poly-G tracts within the coding frames, leading to the hypothesis that shifts in the number of guanosine residues in the poly-G tracts might be responsible for the high frequency variation in structure of gonococcal LOS. We now provide experimental evidence confirming this hypothesis.

scholarly journals Multilocus Sequence Analysis of the Marine Bacterial Genus Tenacibaculum Suggests Parallel Evolution of Fish Pathogenicity and Endemic Colonization of Aquaculture Systems

2014 ◽  
Vol 80 (17) ◽  
pp. 5503-5514 ◽  
Author(s):  
Christophe Habib ◽  
Armel Houel ◽  
Aurélie Lunazzi ◽  
Jean-François Bernardet ◽  
Anne Berit Olsen ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Parallel Evolution ◽  
Phylogenetic Reconstruction ◽  
Multilocus Sequence Analysis ◽  
Fish Pathogens ◽  
Long Distance ◽  
Marine Aquaculture ◽  
Content Type ◽  
The Family ◽  
Cohesive Group

ABSTRACTThe genusTenacibaculum, a member of the familyFlavobacteriaceae, is an abundant component of marine bacterial ecosystems that also hosts several fish pathogens, some of which are of serious concern for marine aquaculture. Here, we applied multilocus sequence analysis (MLSA) to 114 representatives of most known species in the genus and of the worldwide diversity of the major fish pathogenTenacibaculum maritimum. Recombination hampers precise phylogenetic reconstruction, but the data indicate intertwined environmental and pathogenic lineages, which suggests that pathogenicity evolved independently in several species. At lower phylogenetic levels recombination is also important, and the speciesT. maritimumconstitutes a cohesive group of isolates. Importantly, the data reveal no trace of long-distance dissemination that could be linked to international fish movements. Instead, the high number of distinct genotypes suggests an endemic distribution of strains. The MLSA scheme and the data described in this study will help in monitoringTenacibaculuminfections in marine aquaculture; we show, for instance, that isolates from tenacibaculosis outbreaks in Norwegian salmon farms are related toT. dicentrarchi, a recently described species.

scholarly journals The Tolerance of Salinity in Rice Requires the Presence of a Functional Copy of FLN2

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 17 ◽  
Author(s):  
Guang Chen ◽  
Jiang Hu ◽  
Liuliu Dong ◽  
Dali Zeng ◽  
Longbiao Guo ◽  
...  
Keyword(s):  
Salinity Stress ◽  
Salinity Tolerance ◽  
Sugar Metabolism ◽  
Invertase Activity ◽  
Leaf Sheath ◽  
Japonica Rice ◽  
Wild Type ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding ◽  
Knockout Line

A panel of ethane-methyl-sulfonate-mutagenized japonica rice lines was grown in the presence of salinity in order to identify genes required for the expression of salinity tolerance. A highly nontolerant selection proved to harbor a mutation in FLN2, a gene which encodes fructokinase-like protein2. Exposure of wild-type rice to salinity up-regulated FLN2, while a CRISPR/Cas9-generated FLN2 knockout line was hypersensitive to the stress. Both ribulose 1,5-bisphosphate carboxylase/oxygenase activity and the abundance of the transcript generated by a number of genes encoding components of sucrose synthesis were lower in the knockout line than in wild-type plants’ leaves, while the sucrose contents of the leaf and root were, respectively, markedly increased and decreased. That sugar partitioning to the roots was impaired in FLN2 knockout plants was confirmed by the observation that several genes involved in carbon transport were down-regulated in both the leaf and in the leaf sheath. The levels of sucrose synthase, acid invertase, and neutral invertase activity were distinctly lower in the knockout plants’ roots than in those of wild-type plants, particularly when the plants were exposed to salinity stress. The compromised salinity tolerance exhibited by the FLN2 knockout plants was likely a consequence of an inadequate supply of the assimilate required to support growth, a problem which was rectifiable by providing an exogenous supply of sucrose. The conclusion was that FLN2, on account of its influence over sugar metabolism, is important in the context of seedling growth and the rice plant’s response to salinity stress.

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