In situ measurement of oxygen consumption rate in the bottom layer in Mikawa Bay

1985 ◽  
Vol 41 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Sanae Unoki ◽  
Yatsuka Saijo ◽  
Sukemoto Tawara
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Bottom Layer ◽  
In Situ Measurement

scholarly journals Sulfide may directly modify cytoplasmic hemoglobin deoxygenation in Solemya reidi gills

1996 ◽  
Vol 199 (6) ◽  
pp. 1343-1352 ◽  
Author(s):  
D Kraus ◽  
J Doeller ◽  
C Powell
Keyword(s):  
Oxygen Consumption ◽  
Hydrogen Sulfide ◽  
Intracellular Ph ◽  
Oxygen Consumption Rate ◽  
Optical Spectrum ◽  
Consumption Rate ◽  
Dissociation Rate ◽  
High Concentration ◽  
Dissociation Rate Constants

The clam Solemya reidi, which survives in sulfide-rich sediments, houses intracellular sulfide-oxidizing bacteria as symbionts in its gills. The gill bacteriocytes also contain a high concentration of cytoplasmic hemoglobin. Although the in situ hemoglobin optical spectrum was not altered in the presence of hydrogen sulfide, hemoglobin deoxygenation was significantly slowed and incomplete when sulfide was present. A sulfide-mediated decrease in oxygen consumption rate, a shift in intracellular pH or the conversion of hemoglobin to an unusual derivative could all slow in situ hemoglobin deoxygenation. However, under low sulfide levels at which deoxygenation is incomplete, oxygen consumption rate was not inhibited, intracellular pH decreased by less than 0.1 units and the only hemoglobin derivatives present were deoxyhemoglobin and oxyhemoglobin. These results and preliminary measurements of the isolated gill hemoglobin dissociation rate constants suggest that sulfide or a rapidly formed oxidation product may directly influence the rate of Solemya reidi gill hemoglobin deoxygenation.

An in situ biofouling monitor for membrane systems

2003 ◽  
Vol 3 (5-6) ◽  
pp. 205-210 ◽  
Author(s):  
J.W.N.M. Kappelhof ◽  
H.S. Vrouwenvelder ◽  
M. Schaap ◽  
J.C. Kruithof ◽  
D. van der Kooij ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Early Stage ◽  
Membrane Systems ◽  
Active Biomass ◽  
Ongoing Research ◽  
Rate Of Oxygen Consumption ◽  
Non Destructive

Biofouling is a frequently occurring fouling mechanism in membrane applications. Controlling this phenomenon is a challenge due to the difficulty of cleaning biofouling in spiral wound membrane elements. It is assumed that cleaning can be more efficient when biofouling is in an early stage of colonisation. Therefore a sensitive method has to be available for an early identification of biofouling. The present method, the measurement of the normalised pressure drop (NPD) is not specific for biofouling and is not very sensitive. In this research the feasibility of the specific oxygen consumption rate for detection of the activity of biofilms was investigated in membrane systems. The method has the advantages to be specific for active biomass, applicable in situ, non-destructive and more sensitive than NPD. Three experiments demonstrated that the measurement of the rate of oxygen consumption is potentially a simple, reliable method for the measurement of the active biomass in membrane systems. During one of the experiments the method illustrated the effect of cleaning and the regrowth of bacteria afterwards. The method will be further evaluated and standardized. The relation between the specific oxygen consumption rate and the condition of the biofilm, measured by autopsy of membrane elements, will be further explored. The ongoing research will result in an apparatus and procedure, to be used for biofouling identification during the operation of full-scale and pilot plants.

scholarly journals Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes

2020 ◽  
Vol 1 (2) ◽  
pp. 100072
Author(s):  
Meng Gao ◽  
Anqi Li ◽  
Yuan Qin ◽  
Bilin Liu ◽  
Guohua Gong
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate

Microbial efficiency in a meromictic reservoir

2008 ◽  
Vol 37 (2) ◽  
Author(s):  
Grażyna Mazurkiewicz-Boroń ◽  
Teresa Bednarz ◽  
Elżbieta Wilk-Woźniak
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Consumption ◽  
Release Rate ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Metabolic Efficiency ◽  
Sulphur Compounds ◽  
Carbon Dioxide Release ◽  
Microbial Efficiency ◽  
Ion Contents

Microbial efficiency in a meromictic reservoirIndices of microbial efficiency (expressed as oxygen consumption and carbon dioxide release) were determined in the water column of the meromictic Piaseczno Reservoir (in an opencast sulphur mine), which is rich in sulphur compounds. Phytoplankton abundances were low in both the mixolimnion (up to 15 m depth) and monimolimnion (below 15 m depth). In summer and winter, carbon dioxide release was 3-fold and 5-fold higher, respectively, in the monimolimnion than in the mixolimnion. Laboratory enrichments of the sulphur substrate of the water resulted in a decrease in oxygen consumption rate of by about 42% in mixolimnion samples, and in the carbon dioxide release rate by about 69% in monimolimnion samples. Water temperature, pH and bivalent ion contents were of major importance in shaping the microbial metabolic efficiency in the mixolimnion, whilst in the monimolimnion these relationships were not evident.

scholarly journals Correction: Paszkiewicz et al. A Peripheral CB1R Antagonist Increases Lipolysis, Oxygen Consumption Rate, and Markers of Beiging in 3T3-L1 Adipocytes Similar to RIM, Suggesting That Central Effects Can Be Avoided. Int. J. Mol. Sci. 2020, 21, 6639

2021 ◽  
Vol 22 (9) ◽  
pp. 4366
Author(s):  
Rebecca L. Paszkiewicz ◽  
Richard N. Bergman ◽  
Roberta S. Santos ◽  
Aaron P. Frank ◽  
Orison O. Woolcott ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Central Effects

The authors wish to make the following corrections to this paper [...]

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

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