scholarly journals Sulfide may directly modify cytoplasmic hemoglobin deoxygenation in Solemya reidi gills

1996 ◽  
Vol 199 (6) ◽  
pp. 1343-1352 ◽  
Author(s):  
D Kraus ◽  
J Doeller ◽  
C Powell

The clam Solemya reidi, which survives in sulfide-rich sediments, houses intracellular sulfide-oxidizing bacteria as symbionts in its gills. The gill bacteriocytes also contain a high concentration of cytoplasmic hemoglobin. Although the in situ hemoglobin optical spectrum was not altered in the presence of hydrogen sulfide, hemoglobin deoxygenation was significantly slowed and incomplete when sulfide was present. A sulfide-mediated decrease in oxygen consumption rate, a shift in intracellular pH or the conversion of hemoglobin to an unusual derivative could all slow in situ hemoglobin deoxygenation. However, under low sulfide levels at which deoxygenation is incomplete, oxygen consumption rate was not inhibited, intracellular pH decreased by less than 0.1 units and the only hemoglobin derivatives present were deoxyhemoglobin and oxyhemoglobin. These results and preliminary measurements of the isolated gill hemoglobin dissociation rate constants suggest that sulfide or a rapidly formed oxidation product may directly influence the rate of Solemya reidi gill hemoglobin deoxygenation.

1984 ◽  
Vol 109 (1) ◽  
pp. 341-352
Author(s):  
J. Mallefet ◽  
F. Baguet

Isolated photophores of Porichthys notatus, maintained in saline at 20 degrees C, do not luminesce and show an oxygen consumption rate of 0.07 +/− 0.01 nmol min-1 photophore-1. In the presence of 10(−6) M-KCN, the photophores do not luminesce but the resting respiration decreases by about 50%. In the presence of 10(−5) M-KCN, some photophores do not luminesce and their respiration rate decreases by about 75%. Others show a response and resting oxygen consumption slowly increases. At high concentration (10(−4) and 10(−3)M), KCN induces a large light emission and increase in oxygen consumption. The stimulatory effect of KCN on the photophore oxygen consumption is tentatively explained by an activation of the luciferin-luciferase system by calcium ions.


2003 ◽  
Vol 3 (5-6) ◽  
pp. 205-210 ◽  
Author(s):  
J.W.N.M. Kappelhof ◽  
H.S. Vrouwenvelder ◽  
M. Schaap ◽  
J.C. Kruithof ◽  
D. van der Kooij ◽  
...  

Biofouling is a frequently occurring fouling mechanism in membrane applications. Controlling this phenomenon is a challenge due to the difficulty of cleaning biofouling in spiral wound membrane elements. It is assumed that cleaning can be more efficient when biofouling is in an early stage of colonisation. Therefore a sensitive method has to be available for an early identification of biofouling. The present method, the measurement of the normalised pressure drop (NPD) is not specific for biofouling and is not very sensitive. In this research the feasibility of the specific oxygen consumption rate for detection of the activity of biofilms was investigated in membrane systems. The method has the advantages to be specific for active biomass, applicable in situ, non-destructive and more sensitive than NPD. Three experiments demonstrated that the measurement of the rate of oxygen consumption is potentially a simple, reliable method for the measurement of the active biomass in membrane systems. During one of the experiments the method illustrated the effect of cleaning and the regrowth of bacteria afterwards. The method will be further evaluated and standardized. The relation between the specific oxygen consumption rate and the condition of the biofilm, measured by autopsy of membrane elements, will be further explored. The ongoing research will result in an apparatus and procedure, to be used for biofouling identification during the operation of full-scale and pilot plants.


2020 ◽  
Vol 1 (2) ◽  
pp. 100072
Author(s):  
Meng Gao ◽  
Anqi Li ◽  
Yuan Qin ◽  
Bilin Liu ◽  
Guohua Gong

Author(s):  
Grażyna Mazurkiewicz-Boroń ◽  
Teresa Bednarz ◽  
Elżbieta Wilk-Woźniak

Microbial efficiency in a meromictic reservoirIndices of microbial efficiency (expressed as oxygen consumption and carbon dioxide release) were determined in the water column of the meromictic Piaseczno Reservoir (in an opencast sulphur mine), which is rich in sulphur compounds. Phytoplankton abundances were low in both the mixolimnion (up to 15 m depth) and monimolimnion (below 15 m depth). In summer and winter, carbon dioxide release was 3-fold and 5-fold higher, respectively, in the monimolimnion than in the mixolimnion. Laboratory enrichments of the sulphur substrate of the water resulted in a decrease in oxygen consumption rate of by about 42% in mixolimnion samples, and in the carbon dioxide release rate by about 69% in monimolimnion samples. Water temperature, pH and bivalent ion contents were of major importance in shaping the microbial metabolic efficiency in the mixolimnion, whilst in the monimolimnion these relationships were not evident.


2021 ◽  
Vol 22 (9) ◽  
pp. 4366
Author(s):  
Rebecca L. Paszkiewicz ◽  
Richard N. Bergman ◽  
Roberta S. Santos ◽  
Aaron P. Frank ◽  
Orison O. Woolcott ◽  
...  

The authors wish to make the following corrections to this paper [...]


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