Gaussian band analysis of absorption, fluorescence and photobleaching difference spectra of D1/D2/cytb-559 complex

1994 ◽  
Vol 41 (3) ◽  
pp. 465-473 ◽  
Author(s):  
Flavio Massimo Garlaschi ◽  
Giuseppe Zucchelli ◽  
Paolo Giavazzi ◽  
Robert Charles Jennings
Keyword(s):  
Difference Spectra ◽  
Gaussian Band

Anomalous Bands in Infrared Difference Spectra Due to Frequency Shifts

1982 ◽  
Vol 36 (5) ◽  
pp. 539-544 ◽  
Author(s):  
Chris W. Brown ◽  
Patricia F. Lynch ◽  
Robert J. Obremski
Keyword(s):  
Molecular Interactions ◽  
Solvent Effects ◽  
Experimental Error ◽  
Difference Spectra ◽  
Band Shape ◽  
Frequency Shifts ◽  
Gaussian Band

Artifacts appearing in infrared difference spectra can be caused by the lack of wavelength reproducibility and/or molecular interactions such as solvent effects. These artifacts appear as derivatives in difference spectra. Experimental spectra containing a number of bands with a variety of bandwidths were shifted over a range of frequencies and subtracted from the original spectra to intentionally produce anomalous bands. Models based on both Lorentzian and Gaussian band shapes were used to fit the observed data, and it was found that either band shape could be used to adequately predict the observed results within the experimental error of the measurements. Moreover, the models can be used to determine the values of the frequency shifts from the peak-to-peak differences of artifacts.

scholarly journals Ultraviolet Difference Spectra of Tyrosine Groups in Proteins and Amino Acids

1958 ◽  
Vol 233 (6) ◽  
pp. 1421-1428 ◽  
Author(s):  
Donald B. Wetlaufer ◽  
John T. Edsall ◽  
Barbara R. Hollingworth
Keyword(s):  
Amino Acids ◽  
Difference Spectra

scholarly journals Studies on the Ultraviolet Difference Spectra of Proteins and Polypeptides

1961 ◽  
Vol 236 (11) ◽  
pp. 2942-2947 ◽  
Author(s):  
A.N. Glazer ◽  
Emil L. Smith
Keyword(s):  
Difference Spectra

In Situ Diagnostics of Vuv Laser Cvd of Semiconductor Interfaces by Ftir Spectroscopy and Spectroscopic Ellipsometry

1995 ◽  
Vol 397 ◽  
Author(s):  
M. Barth ◽  
J. Knobloch ◽  
P. Hess
Keyword(s):  
Film Thickness ◽  
Bulk Material ◽  
Substrate Surface ◽  
Cluster Formation ◽  
Interface Layer ◽  
Native Oxide ◽  
Initial Growth ◽  
Difference Spectra ◽  
Si Substrates

ABSTRACTThe growth of high quality amorphous hydrogenated semiconductor films was explored with different in situ spectroscopic methods. Nucleation of ArF laser-induced CVD of a-Ge:H on different substrates was investigated by real time ellipsometry, whereas the F2 laser (157nm) deposition of a-Si:H was monitored by FTIR transmission spectroscopy. The ellipsometric studies reveal a significant influence of the substrate surface on the nucleation stage, which in fact determines the electronic and mechanical properties of the bulk material. Coalescence of initial clusters occurs at a thickness of 16 Å for atomically smooth hydrogen-terminated c-Si substrates, whereas on native oxide covered c-Si substrates the bulk volume void fractions are not reached until 35 Å film thickness. For the first time we present a series of IR transmission spectra with monolayer resolution of the initial growth of a-Si:H. Hereby the film thickness was measured simultaneously using a quartz crystal microbalance with corresponding sensitivity. The results give evidence for cluster formation with a coalescence radius of about 20 Å. Difference spectra calculated for layers at different depths with definite thickness reveal that the hydrogen-rich interface layer stays at the substrate surface and does not move with the surface of the growing film. The decrease of the Urbach energy switching from native oxide to H-terminated substrates suggests a strong influence of the interface morphology on the bulk material quality.

scholarly journals Detection and assignment of the glucose signal in1h nmr difference spectra of the human brain

1992 ◽  
Vol 27 (1) ◽  
pp. 183-188 ◽  
Author(s):  
Rolf Gruetter ◽  
Douglas L. Rothman ◽  
Edward J. Novotny ◽  
Gerald I. Shulman ◽  
James W. Prichard ◽  
...  
Keyword(s):  
Human Brain ◽  
Difference Spectra

The interaction of bovine milk caseins with the detergent sodium dodecyl sulphate. II. The effect of detergent binding on spectral properties of caseins

1970 ◽  
Vol 37 (2) ◽  
pp. 259-267 ◽  
Author(s):  
G. C. Cheeseman ◽  
Dorothy J. Knight
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Bovine Milk ◽  
Difference Spectrum ◽  
Temperature Dependent ◽  
Difference Spectra ◽  
Negative Change ◽  
Tryptophan Residues ◽  
Hydrophobic Binding ◽  
The Difference

SummaryThe dissociation of casein aggregates by the detergent sodium dodecyl sulphate (SDS) gave rise to difference spectra and these spectra were characteristic for each of the different types of casein. Increase in absorption by the chromophore groups, tyrosine and tryptophan, when αs1- and β-casein aggregates were dissociated indicated binding of the detergent at regions of the molecule containing these residues. A decrease in absorption when κ-casein was dissociated indicated that the tyrosine and tryptophan residues were not in the region of the molecule to which the detergent was bound and that in the κ-casein aggregate these residues were in a more hydrophobic environment. Peaks on the difference spectra were obtained at 280 and 288 nm for αs1-casein and 284 and 291 nm for β-casein and troughs at 278 and 286 nm for κ-casein. The difference spectrum reached a maximum value when the αsl- and β-casein aggregates were dissociated and the further binding of SDS did not alter this value. The large negative change in the difference spectrum of κ-casein did not occur until after most of the aggregates were dissociated and did not reach a maximum until binding with SDS was complete. The value obtained for ΔOD was found to be temperature-dependent for β-casein-SDS interaction, but not for αs1- and κ-casein. Changes in spectra were also observed when αs1- and κ-casein interacted to form aggregates. The data obtained confirmed the importance of hydrophobic binding in casein aggregate formation and indicated the possible involvement of tyrosine and tryptophan residues in this binding.

State of Water in Supersaturated Nitrate Aerosols Disclosed by the Raman Difference Spectra

2009 ◽  
Vol 113 (1) ◽  
pp. 215-220 ◽  
Author(s):  
Li-Jun Zhao ◽  
Qing-Xuan Zeng ◽  
Yun-Hong Zhang
Keyword(s):  
Difference Spectra ◽  
State Of Water

The isolation, structure, and stereochemistry of traversiadiene. The precursor hydrocarbon of traversianal biosynthesis

1989 ◽  
Vol 67 (8) ◽  
pp. 1302-1304 ◽  
Author(s):  
Albert Stoessl ◽  
G. L. Rock ◽  
J. B. Stothers
Keyword(s):  
Magnetic Resonance ◽  
Biosynthetic Pathway ◽  
Nuclear Overhauser Effect ◽  
Difference Spectra ◽  
Heteronuclear Correlation ◽  
Magnetic Resonance Studies ◽  
Overhauser Effect ◽  
Effect Difference ◽  
Nuclear Overhauser ◽  
Magnetic Resonance Spectra

A tricyclic diene, traversiadiene, isolated from cultures of Cercosporatraversiana has been shown to have the structure and stereochemistry of the previously postulated hydrocarbon intermediate on the biosynthetic pathway to traversianal (1). Detailed:1H and 13C magnetic resonance studies, including homo- and heteronuclear correlation spectra, led to the gross structure, and the stereochemistry was established through a series of nuclear Overhauser effect difference spectra. Keywords: diterpene, traversiadiene, 1H and 13C magnetic resonance spectra.

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