T-cell subsets and T-cell receptor Vβ utilization by Igh-1-congenic mice in herpetic retinal necrosis

1996 ◽  
Vol 234 (S1) ◽  
pp. S83-S88 ◽  
Author(s):  
Alejandro Berra ◽  
Arnd Heiligenhaus ◽  
C. Stephen Foster
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
Congenic Mice ◽  
T Cell Receptor Vβ

scholarly journals Simplified Fluorescent Multiplex PCR Method for Evaluation of the T-Cell Receptor Vβ-Chain Repertoire

2005 ◽  
Vol 12 (4) ◽  
pp. 477-483 ◽  
Author(s):  
Sanjit Fernandes ◽  
Surendra Chavan ◽  
Vivek Chitnis ◽  
Nina Kohn ◽  
Savita Pahwa
Keyword(s):  
T Cell ◽  
Multiplex Pcr ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
Clinical Samples ◽  
Cdr3 Length ◽  
Pcr Products ◽  
Pcr Method ◽  
T Cell Receptor Vβ

ABSTRACTRationale: evaluation of the T-cell receptor (TCR) Vβ-chain repertoire by PCR-based CDR3 length analysis allows fine resolution of the usage of the TCR Vβ repertoire and is a sensitive tool to monitor changes in the T-cell compartment. A multiplex PCR method employing 24 labeled upstream Vβ primers instead of the conventionally labeled downstream Cβ primer is described. Method: RNA was isolated from purified CD4 and CD8 T-cell subsets from umbilical cord blood and clinical samples using TRI reagent followed by reverse transcription using a Cβ primer and an Omniscript RT kit. The 24 Vβ primers were multiplexed based on compatibility and product sizes into seven reactions. cDNA was amplified using 24 Vβ primers (labeled with tetrachloro-6-cardoxyfluorescein, 6-carboxyfluorescein, and hexachloro-6-carboxyfluorescein), an unlabeled Cβ primer, and Taqgold polymerase. The fluorescent PCR products were resolved on an automated DNA sequencer and analyzed using the Genotyper 2.1 software. Results: Vβ spectratypes of excellent resolution were obtained with RNA amounts of 250 ng using the labeled Vβ primers. The resolution was superior to that obtained with the labeled Cβ primer assay. Also the numbers of PCRs were reduced to 7 from the 12 required in the Cβ labeling method, and the sample processing time was reduced by half. Conclusion: The method described for T-cell receptor Vβ-chain repertoire analysis eliminates tedious dilutions and results in superior resolution with small amounts of RNA. The fast throughput makes this method suitable for automation and offers the feasibility to perform TCR Vβ repertoire analyses in clinical trials.

scholarly journals Overexpression of Select T Cell Receptor Vβ Gene Families within CD4+ and CD8+ T Cell Subsets of Myasthenia Gravis Patients: A Role for Superantigen(s)?

1996 ◽  
Vol 2 (4) ◽  
pp. 452-459 ◽  
Author(s):  
Dulceaydee Gigliotti ◽  
Ann-Kari Lefvert ◽  
Mahmood Jeddi-Tehrani ◽  
Semih Esin ◽  
Vida Hodara ◽  
...  
Keyword(s):  
T Cell ◽  
Myasthenia Gravis ◽  
T Cell Receptor ◽  
Gene Families ◽  
Cd8 T Cell ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
T Cell Receptor Vβ

Selection of T Lymphocytes in two Rheumatoid Arthritis Patients Defines Different T-Cell Receptor Vβ Repertoires in CD4+ and CD8+ T-Cell Subsets

1993 ◽  
Vol 6 (5) ◽  
pp. 621-637 ◽  
Author(s):  
Alessandra Sottini ◽  
Luisa Imberti ◽  
Alessandra Bettinardi ◽  
Cinzia Mazza ◽  
Roberto Gorla ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Cd8 T Cell ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
T Cell Receptor Vβ ◽  
Selection Of

Mucosal T cell subsets in coeliac disease: Expression of T cell receptor and CD45 isoforms

1991 ◽  
Vol 10 (3-4) ◽  
pp. 493-496 ◽  
Author(s):  
Trond S. Halstensen ◽  
Per Brandtzaeg
Keyword(s):  
T Cell ◽  
Coeliac Disease ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
Disease Expression ◽  
Cd45 Isoforms

Selection for T-cell receptor Vβ–Dβ–Jβ gene rearrangements with specificity for a myelin basic protein peptide in brain lesions of multiple sclerosis

Nature ◽  
1993 ◽  
Vol 362 (6415) ◽  
pp. 68-70 ◽  
Author(s):  
Jorge R. Oksenberg ◽  
Michael A. Panzara ◽  
Ann B. Begovich ◽  
Dennis Mitchell ◽  
Henry A. Erlich ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
T Cell ◽  
Myelin Basic Protein ◽  
T Cell Receptor ◽  
Basic Protein ◽  
Cell Receptor ◽  
Brain Lesions ◽  
Gene Rearrangements ◽  
Selection For ◽  
T Cell Receptor Vβ

Selective engraftment of memory CD4+ T cells with an unusual recirculation pattern and a diverse T cell receptor-Vβ repertoire into scid mice

1993 ◽  
Vol 23 (2) ◽  
pp. 350-356 ◽  
Author(s):  
Jörg Reimann ◽  
Angelika Rudoiphi ◽  
Thomas Tscherning ◽  
Mogens H. Claesson
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cd4 T Cells ◽  
Cell Receptor ◽  
Scid Mice ◽  
Memory Cd4 T Cells ◽  
T Cell Receptor Vβ

T cell receptor-γδ-expressing fetal mouse thymocytes are generated without T cell receptor Vβ selection

1996 ◽  
Vol 26 (4) ◽  
pp. 804-810 ◽  
Author(s):  
Elisabeth Mertsching ◽  
Rhodri Ceredig
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Fetal Mouse ◽  
T Cell Receptor Vβ
Sign in / Sign up

Export Citation Format

Share Document