Use ofNicotiana debneyi to detect viruses S, X and Y in potato seed stocks and relative susceptibility of six common varieties to potato virus S

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.

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Macroarray Detection of Eleven Potato-Infecting Viruses and Potato spindle tuber viroid

Plant Disease ◽

10.1094/pdis-92-5-0730 ◽

2008 ◽

Vol 92 (5) ◽

pp. 730-740 ◽

Cited By ~ 34

Author(s):

Bright Agindotan ◽

Keith L. Perry

Keyword(s):

Mosaic Virus ◽

Potato Virus ◽

Tobacco Rattle Virus ◽

Alfalfa Mosaic Virus ◽

Potato Virus X ◽

Potato Spindle Tuber Viroid ◽

Apparent Competition ◽

Enzyme Linked Immunosorbent Assay ◽

Potato Seed ◽

Potato Virus S

A macroarray was developed for the detection of 11 potato viruses and Potato spindle tuber viroid. The 11 viruses detected included those commonly found or tested for in North American potato seed certification programs: Alfalfa mosaic virus, Cucumber mosaic virus, Potato mop top virus, Potato leafroll virus, Potato latent virus, Potato virus A, Potato virus M, Potato virus S, Potato virus X, Potato virus Y, and Tobacco rattle virus. These viruses were detected using oligonucleotide 70-mer probes and labeled targets prepared by a random primed amplification procedure. Potato plants analyzed included those infected with 12 reference virus stocks and 36 field isolates. Results from the macroarray were entirely consistent with those obtained using a standard serological assay (enzyme-linked immunosorbent assay). Four isolates of Potato spindle tuber viroid, in mixed infection with one or more viruses, also were detected in the array, although strong hybridization signals required amplification with viroid-specific primers in combination with anchored-random primers. In individual plants, up to four viruses, or a viroid plus two viruses, were detected, with no apparent competition or inhibition. Macroarrays are a cost-effective approach to the simultaneous diagnostic detection of multiple pathogens from infected plants.

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Strain Characterization of Potato virus S Isolates from Tasmania, Australia

Plant Disease ◽

10.1094/pdis-07-11-0573 ◽

2012 ◽

Vol 96 (6) ◽

pp. 813-819 ◽

Cited By ~ 11

Author(s):

Susan J. Lambert ◽

Jason B. Scott ◽

Sarah J. Pethybridge ◽

Frank S. Hay

Keyword(s):

Potato Virus ◽

Chenopodium Quinoa ◽

Protein Sequencing ◽

Potato Seed ◽

Serological Detection ◽

Potato Virus S ◽

Strain Characterization ◽

Geographical Regions ◽

Local Lesions

Potato virus S (PVS) is prevalent within potato (Solanum tuberosum) production worldwide. Traditionally, PVS has been split into two strains, Ordinary (PVSO) and Andean (PVSA), based on reaction in herbaceous indicator species such as Chenopodium quinoa. However, recent research has identified further strain designations, such as PVSO-CS (Ordinary and Chenopodium systemic). Forty-four isolates of PVS were collected from potato seed lines in different geographical regions within Tasmania, Australia. Isolates were initially characterized by reactions in C. quinoa. Nineteen isolates were characterized as PVSO, based on the development of local lesions and serological detection in inoculated leaves only. Three isolates were identified as PVSA-like, based on local lesion development in inoculated leaves, mild mottling or chlorotic spots on noninoculated leaves, and serological detection in both inoculated and noninoculated leaves. Thirteen isolates produced no symptoms, and were detected serologically in inoculated leaves only (PVSO-like). Four isolates produced no symptoms but were detected serologically in both inoculated and noninoculated leaves (PVSA-like). Five isolates produced symptoms in inoculated leaves only but were detected serologically in both inoculated and noninoculated leaves (also PVSA-like). The ability of isolates to infect tomato has also been used as a criterion to assist in PVS strain differentiation. A subsample of isolates (n = 16) was unable to infect tomato ‘Grosse Lisse’. Seventeen isolates representative of these groupings based on reactions in C. quinoa were also characterized by coat-protein sequencing. Phylogenetic comparisons suggested that all isolates were PVSO rather than PVSA. Therefore, whereas some of these PVS isolates were systemic in C. quinoa, findings from this study suggest that they were not PVSA, and that only PVSO and PVSO-CS isolates are present in Tasmania. The implications of this finding for disease management are discussed.

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Prevalence, distribution and control of six major potato viruses in Kenya

Tropical Plant Pathology ◽

10.1007/s40858-020-00409-x ◽

2020 ◽

Author(s):

John Onditi ◽

Moses Nyongesa ◽

René van der Vlugt

Keyword(s):

Potato Virus ◽

Leaf Roll ◽

Potato Virus X ◽

Plant Viruses ◽

Multiple Infections ◽

Potato Seed ◽

Potato Leaf ◽

Potato Virus S ◽

Potato Viruses ◽

Virus Prevalence

AbstractIn most developing countries, farmers lack sufficient supply of certified or healthy potato seed tubers. Hence, they often plant their own saved ware potato tubers, a practice that is known to contribute to spread and increase the prevalence of plant viruses. In this study, we proposed options for managing the virus based on the knowledge obtained from surveys of virus prevalence and distribution in potato cultivars grown under such conditions. Potato leaf samples randomly collected from 354 farms in five major potato-growing counties in Kenya were tested for six potato viruses; potato virus Y (PVY), potato leaf roll virus (PLRV), potato virus X (PVX), potato virus M (PVM), potato virus A (PVA) and potato virus S (PVS) through DAS-ELISA. Virus prevalence in the fields was high; 72.9% of the samples were positive for at least one of the six viruses; and 55.9% showed multiple infections. A follow-up survey conducted during three consecutive seasons, in two of the five counties, revealed that virus prevalence fluctuated across seasons. This suggested that updated information on virus prevalence might be of value for designing a virus control strategy. Distribution maps showed the presence of the viruses restricted to specific geographic regions, an indication of where control efforts should be directed. Four cultivars, Sherekea, Shangi, Kenya Karibu and Asante, grown at a high virus-prevalent area, showed low values of average ELISA absorbance (OD), suggest a field resistance to the viruses. This study demonstrated that knowledge of prevalence and distribution may be of value to identify and recommend virus resistant cultivars to replace susceptible ones, especially in the virus hotspot areas.

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SPREAD OF POTATO VIRAL DISEASE PATHOGENS IN CULTURE CULTIVATION ZONES

Agriciltural microbiology ◽

10.35868/1997-3004.32.67-73 ◽

2021 ◽

Vol 32 ◽

pp. 67-73

Author(s):

І. V. Volkova ◽

L. M. Reshotko ◽

О. О. Dmytruk

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Viral Disease ◽

Viral Diseases ◽

Potato Seed ◽

Moderate Degree ◽

Potato Virus S ◽

Trade Relations ◽

Low Degree ◽

Potato Virus M

Objective. Monitoring observations over the phytovirological condition of potato planting in different regions of Ukraine, taking into account potato degeneration zones. Methods. Laboratory (virological, immunological, electronic microscopy), field, statistical. Results. The map and diagrams show the results of monitoring studies conducted in 2016–2020, during which 435 varieties of potatoes of Ukrainian and foreign selection were tested. In the zone of the lowest degeneration or the zone with a relatively low degree of infectious load, 100 % spread of pathogenic complexes involving potato virus Y in the Region of Zakarpattia, potato viruses M and Y in the Region of Chernivtsi, ranging from 89 % to 100 %. In the zone of strong degeneration of the culture or the zone with a high degree of infectious load, spread of pathogens of potato viral diseases is from 94 % to 100 %, and the vast majority of viruses belongs to pathogens of severe viral diseases. In the zones of mild and moderate degeneration, which belong to the zones with relatively low degree and with moderate degree of infectious load according to another diagram, potato virus M in monoinfection from 17 % to 50 %, in the pathogenic complex with potato virus S from 8 % to 36 %, with potato virus Y from 6 % to 32 % was detected. In the Region of Kyiv, the spread of potato virus Y+S complex reaches 65 %. In all areas, there is an extremely high level of infection of the culture with potato virus M, which is the cause of severe viral diseases of potatoes. Conclusion. The results of phytovirological monitoring show that the boundaries between potato degeneration zones are smoothed down. Climatic changes, active resettlement of virus vectors, intensification of trade relations with the constant import of foreign potato seed material result in the significant spread of pathogens of potato viral diseases.

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Reinfection of potato seed stocks with potato virus S and potato virus X in Wisconsin

American Potato Journal ◽

10.1007/bf02854381 ◽

1981 ◽

Vol 58 (2) ◽

pp. 117-125 ◽

Cited By ~ 11

Author(s):

Y. Hahm ◽

S. A. Slack ◽

R. J. Slattery

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Potato Seed ◽

Potato Virus S

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Distribution and incidence of viruses in Irish seed potato crops

Irish Journal of Agricultural and Food Research ◽

10.1515/ijafr-2015-0011 ◽

2015 ◽

Vol 54 (2) ◽

pp. 98-106 ◽

Cited By ~ 4

Author(s):

F. Hutton ◽

J.H. Spink ◽

D. Griffin ◽

S. Kildea ◽

D. Bonner ◽

...

Keyword(s):

Potato Virus ◽

Significant Proportion ◽

Potato Production ◽

Weather Data ◽

Management Approach ◽

Potato Seed ◽

Virus Diseases ◽

Aphid Control ◽

Daily Weather Data ◽

Potato Crops

Abstract Virus diseases are of key importance in potato production and in particular for the production of disease-free potato seed. However, there is little known about the frequency and distribution of potato virus diseases in Ireland. Despite a large number of samples being tested each year, the data has never been collated either within or across years. Information from all known potato virus testing carried out in the years 2006–2012 by the Department of Agriculture Food and Marine was collated to give an indication of the distribution and incidence of potato virus in Ireland. It was found that there was significant variation between regions, varieties, years and seed classes. A definition of daily weather data suitable for aphid flight was developed, which accounted for a significant proportion of the variation in virus incidence between years. This use of weather data to predict virus risk could be developed to form the basis of an integrated pest management approach for aphid control in Irish potato crops.

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