Cytogenetic and biochemical studies on the nucleolus organizing regions of chromosomes in in vivo and in vitro aging

AGE ◽  
1992 ◽  
Vol 15 (2) ◽  
pp. 41-43 ◽  
Author(s):  
Teimuraz Lezhava ◽  
Nana Dvalishvili
Keyword(s):  
In Vitro Aging ◽  
Biochemical Studies

Cellular Samurai: katanin and the severing of microtubules

2000 ◽  
Vol 113 (16) ◽  
pp. 2821-2827 ◽  
Author(s):  
L. Quarmby
Keyword(s):  
Epithelial Cells ◽  
Essential Role ◽  
Aaa Atpase ◽  
C Elegans ◽  
Microtubule Severing ◽  
Biochemical Studies ◽  
New Evidence

Recent biochemical studies of the AAA ATPase, katanin, provide a foundation for understanding how microtubules might be severed along their length. These in vitro studies are complemented by a series of recent reports of direct in vivo observation of microtubule breakage, which indicate that the in vitro phenomenon of catalysed microtubule severing is likely to be physiological. There is also new evidence that microtubule severing by katanin is important for the production of non-centrosomal microtubules in cells such as neurons and epithelial cells. Although it has been difficult to establish the role of katanin in mitosis, new genetic evidence indicates that a katanin-like protein, MEI-1, plays an essential role in meiosis in C. elegans. Finally, new proteins involved in the severing of axonemal microtubules have been discovered in the deflagellation system of Chlamydomonas.

Effects of in vivo and in vitro aging on the glucocorticoid receptors of cultured articular chondrocytes

1981 ◽  
Vol 5 (8) ◽  
pp. 774 ◽  
Author(s):  
M ADOLPHE ◽  
D BLONDELON ◽  
P JAFFRAY ◽  
C PERRET ◽  
L ZIZINE ◽  
...  
Keyword(s):  
Glucocorticoid Receptors ◽  
Articular Chondrocytes ◽  
In Vitro Aging

Mechanical properties of calcia stabilized zirconia following in vivo and in vitro aging

1975 ◽  
Vol 9 (4) ◽  
pp. 63-66 ◽  
Author(s):  
G. H. Kenner ◽  
W. D. Pasco ◽  
J. T. Frakes ◽  
S. D. Brown
Keyword(s):  
Mechanical Properties ◽  
Stabilized Zirconia ◽  
In Vitro Aging

scholarly journals In Vivo and In Vitro Aging Is Detrimental to Mouse Spermatogonial Stem Cell Function

2010 ◽  
Vol 84 (4) ◽  
pp. 698-706 ◽  
Author(s):  
J. A. Schmidt ◽  
L. K. Abramowitz ◽  
H. Kubota ◽  
X. Wu ◽  
Z. Niu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Function ◽  
Spermatogonial Stem Cell ◽  
In Vitro Aging

Genetic and biochemical studies on perchloroethylene ‘in vitro’ and ‘in vivo’

1983 ◽  
Vol 116 (3-4) ◽  
pp. 323-331 ◽  
Author(s):  
G. Bronzetti ◽  
C. Bauer ◽  
C. Corsi ◽  
R. Del Carratore ◽  
A. Galli ◽  
...  
Keyword(s):  
Biochemical Studies

scholarly journals Is Surface Metastability of Today’s Ceramic Bearings a Clinical Issue?

2021 ◽  
Vol 5 (10) ◽  
pp. 273
Author(s):  
Alessandro Alan Porporati ◽  
Laurent Gremillard ◽  
Jérôme Chevalier ◽  
Rocco Pitto ◽  
Marco Deluca
Keyword(s):  
Raman Spectroscopy ◽  
The Body ◽  
Clinical Issue ◽  
Surface Stability ◽  
In Vitro Aging ◽  
Aging Kinetics ◽  
Many Sources

Recent studies on zirconia-toughened alumina (ZTA) evidenced that in vivo aged implants display a much higher monoclinic zirconia content than expected from in vitro simulations by autoclaving. At the moment, there is no agreement on the source of this discrepancy: Some research groups ascribe it to the effect of mechanical impact shocks, which are generally not implemented in standard in vitro aging or hip walking simulators. Others invoke the effect of metal transfer, which should trigger an autocatalytic reaction in the body fluid environment, accelerating the kinetics of tetragonal-to-monoclinic transformation in vivo. Extrapolations of the aging kinetics from high (autoclave) to in vivo temperature are also often disputed. Last, Raman spectroscopy is by far the preferred method to quantify the amount of monoclinically transformed zirconia. There are, however, many sources of errors that may negatively affect Raman results, meaning that the final interpretation might be flawed. In this work, we applied Raman spectroscopy to determine the monoclinic content in as-received and in vitro aged ZTA hip joint implants, and in one long-term retrieval study. We calculated the monoclinic content with the most used equations in the literature and compared it with the results of X-ray diffraction obtained on a similar probe depth. Our results show, contrary to many previous studies, that the long-term surface stability of ZTA ceramics is preserved. This suggests that the Raman technique does not offer consistent and unique results for the analysis of surface degradation. Moreover, we discuss here that tetragonal-to-monoclinic transformation is also necessary to limit contact damage and wear stripe extension. Thus, the surface metastability of zirconia-containing ceramics may be a non-issue.

scholarly journals VIABILITY INTERACTIONS, IN VIVO ACTIVITY AND THE G6PD POLYMORPHISM IN DROSOPHILA MELANOGASTER

Genetics ◽  
1984 ◽  
Vol 106 (1) ◽  
pp. 95-107
Author(s):  
Walter F Eanes
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Normal Activity ◽  
Thermal Stabilities ◽  
Biochemical Studies ◽  
Biochemical Approach ◽  
Low Activity ◽  
Balancer Chromosome

ABSTRACT Several biochemical studies have suggested that in Drosophila melanogaster the two common allozymes of G6PD differ in their in vitro activities and thermal stabilities. Yet, it remains to be shown that these characterizations reflect actual in vivo differences and are not artifacts of the biochemical approach. In this study it is shown that in vivo activity differences must exist between these two variants. This conclusion arises from the observation that the viability of flies bearing a low activity allele of 6PGD is strongly dependent on the genotype at the G6PD (Zw) locus, whereas no measurable difference in viability can be detected between Zw genotypes in a normal activity 6PGD background. These viability interactions are in the direction predicted by the reported in vitro activities of the allozymes and the proposed deleterious effects of 6-phosphogluconate accumulation.—In addition, a genetic scheme is used that uncouples and quantifies the effects of viability modifiers in the region of the Zw locus, while homogenizing 98% of the X chromosome. The viability of different Zw genotypes is measured by examining whole chromosome viabilities relative to the FM6 balancer chromosome. The advantages of this particular scheme are discussed.

scholarly journals Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Alessandra Stacchiotti ◽  
Francesca Rovetta ◽  
Matteo Ferroni ◽  
Giovanni Corsetti ◽  
Antonio Lavazza ◽  
...  
Keyword(s):  
C2c12 Myotubes ◽  
Chemotherapeutic Drug ◽  
Skeletal Mass ◽  
Transmission Electron ◽  
Biochemical Studies ◽  
Morphological Approach ◽  
Muscle Homeostasis ◽  
Different Tissues

Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia. Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process that, when excessively activated, may trigger protein hypercatabolism and atrophy in skeletal muscle. To visualize autophagy we used confocal and transmission electron microscopy at different times of treatment and doses of CisPt. Moreover we evaluated the effects of taurine, a cytoprotective beta-amino acid able to counteract oxidative stress, apoptosis, and endoplasmic reticulum stress in different tissues and organs. Our microscopic results indicate that autophagy occurs very early in 50 μM CisPt challenged myotubes (4 h–8 h) before overt atrophy but it persists even at 24 h, when several autophagic vesicles, damaged mitochondria, and sarcoplasmic blebbings engulf the sarcoplasm. Differently, 25 mM taurine pretreatment rescues the majority of myotubes size upon 50 μM CisPt at 24 h. Taurine appears to counteract atrophy by restoring regular microtubular apparatus and mitochondria and reducing the overload and the localization of autophagolysosomes. Such a promising taurine action in preventing atrophy needs further molecular and biochemical studies to best define its impact on muscle homeostasis and the maintenance of an adequate skeletal mass in vivo.

scholarly journals Relationship Between In Vivo Age and In Vitro Aging: Assessment of 669 Cell Cultures Derived From Members of The Baltimore Longitudinal Study of Aging

2002 ◽  
Vol 57 (6) ◽  
pp. B239-B246 ◽  
Author(s):  
J. R. Smith ◽  
S. Venable ◽  
T. W. Roberts ◽  
E. J. Metter ◽  
R. Monticone ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Cell Cultures ◽  
In Vitro Aging ◽  
Baltimore Longitudinal Study
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