FLIM on a wide field fluorescence microscope

2003 ◽  
Vol 10 (5-6) ◽  
pp. 501-510 ◽  
Author(s):  
L. K. van Geest ◽  
K. W. J. Stoop
2003 ◽  
Vol 10 (5-6) ◽  
pp. 501-510 ◽  
Author(s):  
L. K. van. Geest ◽  
K. W. J. Stoop

2016 ◽  
Vol 18 (5) ◽  
pp. 209-219 ◽  
Author(s):  
Wolf Heusermann ◽  
Beat Ludin ◽  
Nhan T Pham ◽  
Manfred Auer ◽  
Thomas Weidemann ◽  
...  

1999 ◽  
Vol 7 (4) ◽  
pp. 20-23
Author(s):  
Ted Inoue

If you are one of the many people who are currently considering the purchase of a confocal microscope, you may be asking yourself, "How can 1 be sure that the microscope I choose is the right one?"Before proceeding, take a step back. Ask yourself: "Why use a confocal?" A confocal microscope is, by definition, a microscope which optically "slices" the sample by showing only those details which are at or near the plane of focus. This results in a clearer image than might be possible with a conventional (also called "wide-field") fluorescence microscope - a microscope in which every pixel in every image is corrupted by light from its neighbors. The optical slicing of the sample done by the confocal microscope lets you construct a 3-D representation of the sample that can be very useful for image visualization or analysis.


Author(s):  
M. G. Lagally

It has been recognized since the earliest days of crystal growth that kinetic processes of all Kinds control the nature of the growth. As the technology of crystal growth has become ever more refined, with the advent of such atomistic processes as molecular beam epitaxy, chemical vapor deposition, sputter deposition, and plasma enhanced techniques for the creation of “crystals” as little as one or a few atomic layers thick, multilayer structures, and novel materials combinations, the need to understand the mechanisms controlling the growth process is becoming more critical. Unfortunately, available techniques have not lent themselves well to obtaining a truly microscopic picture of such processes. Because of its atomic resolution on the one hand, and the achievable wide field of view on the other (of the order of micrometers) scanning tunneling microscopy (STM) gives us this opportunity. In this talk, we briefly review the types of growth kinetics measurements that can be made using STM. The use of STM for studies of kinetics is one of the more recent applications of what is itself still a very young field.


Author(s):  
Brian Cross

A relatively new entry, in the field of microscopy, is the Scanning X-Ray Fluorescence Microscope (SXRFM). Using this type of instrument (e.g. Kevex Omicron X-ray Microprobe), one can obtain multiple elemental x-ray images, from the analysis of materials which show heterogeneity. The SXRFM obtains images by collimating an x-ray beam (e.g. 100 μm diameter), and then scanning the sample with a high-speed x-y stage. To speed up the image acquisition, data is acquired "on-the-fly" by slew-scanning the stage along the x-axis, like a TV or SEM scan. To reduce the overhead from "fly-back," the images can be acquired by bi-directional scanning of the x-axis. This results in very little overhead with the re-positioning of the sample stage. The image acquisition rate is dominated by the x-ray acquisition rate. Therefore, the total x-ray image acquisition rate, using the SXRFM, is very comparable to an SEM. Although the x-ray spatial resolution of the SXRFM is worse than an SEM (say 100 vs. 2 μm), there are several other advantages.


2013 ◽  
Vol 183 (8) ◽  
pp. 888-894
Author(s):  
G.M. Beskin ◽  
S.V. Karpov ◽  
V.L. Plokhotnichenko ◽  
S.F. Bondar ◽  
A.V. Perkov ◽  
...  

2010 ◽  
Vol 180 (4) ◽  
pp. 424 ◽  
Author(s):  
G.M. Beskin ◽  
S.V. Karpov ◽  
S.F. Bondar ◽  
V.L. Plokhotnichenko ◽  
A. Guarnieri ◽  
...  

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