Overproduction of alfalfa glutamine synthetase in transgenic tobacco plants

Glutamine synthetase (GS, CE 6.3.1.2) is an enzyme that catalyzes the ATP-dependent condensation of glutamic acid with ammonia to yield glutamine. Glutamine synthetase is a key enzyme involved in the assimilation of inorganic nitrogen into organic forms. In plant cells, GS is present in both chloroplasts (GS2) and cytoplasm (GS1), in which GS1 can assimilate nitrogen source. In this study, one transgenic vector pBI121 carrying GS1 gene under the control of promoter 35S (pBI121::GS1) were successfully constructed. This vector containing G1S gene was transformed into tobacco leaves pieces via Agrobacterium tumefaciens strain C58. Five weeks after cultivation, there were 28 tobacco lines which had roots on the medium added kanamycin 50 mg/l. Then, the presence of G1S gene in these tobacco lines was tested from leaves in the next experiments. PCR and Southern blot confirmed that there are five tobacco lines carrying transferred GS1 gene. The effectiveness of nitrogen using in GS1 transgenic tobacco plants in vitro was evaluated. The tissue fresh weight, number and height of shoots forming buds and rooting ability of GS1 transgenic tobacco plants were greater than those of non-GM plants in the medium of low nitrogen concentration (0.1X - 0.2X). Assessment of crop growing in a greenhouse demonstrated that GS1 transgenic tobacco plants grow faster than non-transgenic ones. In detail, the increment of plant height after planting 03 months and 05 months in greenhouse is 43.55% and 33.29%, respectively. These results provide a scientific basis for the development of other genetically modified plants which enhanced nitrogen-use efficiency.

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The Promoter of the Gene for Glutamine Synthetase from Rice Shows Organ-Specific and Substrate-Induced Expression in Transgenic Tobacco Plants

Plant and Cell Physiology ◽

10.1093/oxfordjournals.pcp.a078087 ◽

1991 ◽

Vol 32 (3) ◽

pp. 353-358 ◽

Cited By ~ 17

Author(s):

Akiko Kozaki ◽

Atsushi Sakamoto ◽

Kunisuke Tanaka ◽

Go Takeba

Keyword(s):

Glutamine Synthetase ◽

Transgenic Tobacco ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Induced Expression ◽

Organ Specific

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Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants

Acta Botanica Yunnanica ◽

10.3724/sp.j.1143.2008.00457 ◽

2009 ◽

Vol 30 (4) ◽

pp. 457-463

Author(s):

Yi-Qun WANG

Keyword(s):

Transgenic Tobacco ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Alpha Lactalbumin

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Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

Transgenic Research ◽

10.1007/s11248-004-8082-8 ◽

2005 ◽

Vol 14 (3) ◽

pp. 251-259 ◽

Cited By ~ 21

Author(s):

Helga Schinkel ◽

Andreas Schiermeyer ◽

Raphael Soeur ◽

Rainer Fischer ◽

Stefan Schillberg

Keyword(s):

Transgenic Tobacco ◽

Transgenic Tobacco Plants ◽

Suspension Cells ◽

Tobacco Plants ◽

Recombinant Thrombomodulin

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Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-021-02040-3 ◽

2021 ◽

Author(s):

Ai-Hua Wang ◽

Lan Yang ◽

Xin-Zhuan Yao ◽

Xiao-Peng Wen

Keyword(s):

Drought Stress ◽

Stress Response ◽

Transgenic Plants ◽

Drought Tolerance ◽

Transgenic Tobacco ◽

Sequence Similarity ◽

Amino Acid Sequence Similarity ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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