Molecular imprinting for chiral separations and drug screening purposes using monolithic stationary phases in CEC

1999 ◽  
Vol 49 (S1) ◽  
pp. S93-S94 ◽  
Author(s):  
L. Schweitz ◽  
L. I. Andersson ◽  
S. Nilsson
Keyword(s):  
Molecular Imprinting ◽  
Drug Screening ◽  
Stationary Phases ◽  
Chiral Separations ◽  
Monolithic Stationary Phases

scholarly journals Recent Advances in Chiral Analysis of Proteins and Peptides

Separations ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 112
Author(s):  
Marine Morvan ◽  
Ivan Mikšík
Keyword(s):  
Amino Acids ◽  
Stationary Phases ◽  
Enzymatic Digestion ◽  
Chiral Separations ◽  
Biological Compounds ◽  
Health And Disease ◽  
Electrophoretic Techniques ◽  
A Site ◽  
Future Work ◽  
Proteins And Peptides

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain D-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with D-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer's disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of D-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations.

Chiral separations of metoprolol and some analogs with carbon dioxide on chiralcel OD and chiralpak AD stationary phases. Use of chemometrics

2000 ◽  
Vol 51 (5-6) ◽  
pp. 283-293 ◽  
Author(s):  
S. Svensson ◽  
A. Karlsson ◽  
O. Gyllenhaal ◽  
J. Vessman
Keyword(s):  
Carbon Dioxide ◽  
Stationary Phases ◽  
Chiral Separations ◽  
Chiralcel Od

Electrochromatography in poly(dimethyl)siloxane microchips using organic monolithic stationary phases

2007 ◽  
Vol 28 (11) ◽  
pp. 1668-1673 ◽  
Author(s):  
Karine Faure ◽  
Maximilien Blas ◽  
Omar Yassine ◽  
Nathalie Delaunay ◽  
Gérard Crétier ◽  
...  
Keyword(s):  
Stationary Phases ◽  
Dimethyl Siloxane ◽  
Monolithic Stationary Phases

scholarly journals Chiral separations of mandelic acid by HPLC using molecularly imprinted polymers

2005 ◽  
Vol 30 (4) ◽  
pp. 67-73 ◽  
Author(s):  
Chin-Yin Hung ◽  
Han-Hung Huang ◽  
Ching-Chiang Hwang
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Mandelic Acid ◽  
Occupational Exposures ◽  
Bulk Polymerization ◽  
Chiral Separations ◽  
Phenyl Acetic Acid ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Chromatographic Resolution

Styrene is used in a variety of chemical industries. Environmental and occupational exposures to styrene occur predominantly through inhalation. The major metabolite of styrene is present in two enantiomeric forms, chiral R- and S- hydroxy-1-phenyl-acetic acid (R-and S-mandelic acid, MA). Thus, the concentration of MA, particularly of its enantiomers, has been used in urine tests to determine whether workers have been exposed to styrene. This study describes a method of analyzing mandelic acid using molecular imprinting techniques and HPLC detection to perform the separation of diastereoisomers of mandelic acid. The molecularly imprinted polymer (MIP) was prepared by non-covalent molecular imprinting using (+) MA, (-) MA or (+) phenylalanine, (-) phenylalanine as templates. Methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) were copolymerized in the presence of the template molecules. The bulk polymerization was carried out at 4ºC under UV radiation. The resulting MIP was grounded into 25~44¼m particles, which were slurry packed into analytical columns. After the template molecules were removed, the MIP-packed columns were found to be effective for the chromatographic resolution of (±)-mandelic acid. This method is simpler and more convenient than other chromatographic methods.

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