pH gradient electrophoresis and isoelectric focusing of lipoproteins on agarose bead thin layers

Lipids ◽  
1979 ◽  
Vol 14 (10) ◽  
pp. 864-871 ◽  
Author(s):  
A. Vost ◽  
D. M. -E. Pocock ◽  
S. Pleet
Keyword(s):  
Isoelectric Focusing ◽  
Thin Layers ◽  
Ph Gradient ◽  
Gradient Electrophoresis ◽  
Agarose Bead

Resolution of Basic Gonococcal Outer Membrane Proteins by Nonequilibrium pH Gradient Electrophoresis

1980 ◽  
Vol 30 (3) ◽  
pp. 773-780
Author(s):  
Robert B. Jones ◽  
Patricia A. Jemison ◽  
Wilbert J. Newhall ◽  
Richard A. Haak
Keyword(s):  
Molecular Weight ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Isoelectric Focusing ◽  
Outer Membrane Protein ◽  
Outer Membrane Proteins ◽  
Sodium Dodecyl ◽  
Ph Gradient ◽  
Gradient Electrophoresis

Outer membrane proteins from opaque and transparent colonial variants of strain F62 of Neisseria gonorrhoeae were analyzed by two-dimensional electrophoresis with isoelectric focusing in the first dimension and sodium dodecyl sulphate-polyacrylamide gel electrophoresis in the second. Most of the higher-molecular-weight proteins focused sharply in the acidic region of the gel. In contrast, the principal outer membrane protein, a 31,000-molecular-weight protein, and the opacity-associated proteins remained near the origin (at the basic end of the gel) without focusing. However, when the samples were loaded on the acidic end of an isoelectric focusing gel and subjected to nonequilibrium pH gradient electrophoresis, these proteins behaved as basic proteins. In addition, three distinct opacity-associated heat-modifiable proteins could be identified. No other differences in the protein composition of outer membranes from opaque and transparent variants were apparent. Amino acid analysis of the principal outer membrane protein indicated that its net positive charge may result from partial amidation of its acidic residues. The unexpected observation that the major surface proteins of the gonococcus are basic may have implications for intragonococcal adhesion and for gonococcal interactions with mammalian cells.

Instability and non-linearity of the pH gradient formed in isoelectric focusing

1979 ◽  
Vol 174 (1) ◽  
pp. 1-11 ◽  
Author(s):  
A. Murel ◽  
I. Kirjanen ◽  
O. Kirret
Keyword(s):  
Isoelectric Focusing ◽  
Ph Gradient

Focusing of Proteins in a Horseshoe Microchannel

2008 ◽  
Author(s):  
Jaesool Shim ◽  
Prashanta Dutta ◽  
Cornelius F. Ivory
Keyword(s):  
Capillary Electrophoresis ◽  
Electric Field ◽  
Isoelectric Focusing ◽  
Ph Gradient ◽  
Complex Geometries ◽  
Electrokinetic Phenomena ◽  
Double Peaks ◽  
Zone Electrophoresis ◽  
Carrier Ampholytes ◽  
Ph Range

Ampholyte based isoelectric focusing (IEF) simulation was conducted to study dispersion of proteins in a horseshoe microchannel. Four model proteins (pls = 6.49, 7.1, 7.93 and 8.6) are focused in a 1 cm long horseshoe channel under an electric field of 300 V/cm. The pH gradient is formed in the presence of 25 biprotic carrier ampholytes (ΔpK = 3.0) within a pH range of 6 to 9. The proteins are focused at 380 sec in a nominal electric field of 300 V/cm. Our numerical results show that the band dispersions of a protein are large during the marching stage, but the dispersions are significantly reduced when the double peaks start to merge. This rearrangement of spreading band is very unique compared to linear electrokinetic phenomena (capillary electrophoresis, zone electrophoresis or electroosmosis) and is independent of channel position and channel shape. Hence, one can perform IEF in complex geometries without incorporating hyperturns.

Isoelectric Focusing in Infinite-Component Mixtures. Creation of a pH Gradient

1989 ◽  
pp. 181-210
Author(s):  
V. G. Babskii ◽  
M. Yu. Zhukov ◽  
V. I. Yudovich
Keyword(s):  
Isoelectric Focusing ◽  
Ph Gradient ◽  
Infinite Component

Differential Radioactive Proteomic Analysis of Microdissected Renal Cell Carcinoma Tissue by 54 cm Isoelectric Focusing in Serial Immobilized pH Gradient Gels

2005 ◽  
Vol 4 (6) ◽  
pp. 2117-2125 ◽  
Author(s):  
Slobodan Poznanović ◽  
Wojciech Wozny ◽  
Gerhard P. Schwall ◽  
Chaturvedula Sastri ◽  
Christian Hunzinger ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Isoelectric Focusing ◽  
Proteomic Analysis ◽  
Renal Cell ◽  
Ph Gradient ◽  
Carcinoma Tissue ◽  
Renal Cell Carcinoma Tissue

Study on stability mechanism of immobilized pH gradient in isoelectric focusing via the Svensson–Tiselius differential equation and moving reaction boundary

Talanta ◽  
2013 ◽  
Vol 111 ◽  
pp. 20-27 ◽  
Author(s):  
Chen-Gang Guo ◽  
Si Li ◽  
Hou-Yu Wang ◽  
Dong Zhang ◽  
Guo-Qing Li ◽  
...  
Keyword(s):  
Differential Equation ◽  
Isoelectric Focusing ◽  
Ph Gradient
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